Tribulus terrestris Protects against Male Reproductive Damage Induced by Cyclophosphamide in Mice

Oxidative Medicine and Cellular Longevity - Tập 2018 Số 1 - 2018
Natasha Frasson Pavin1, Aryele Pinto Izaguirry1, Melina Bucco Soares1, Cristiano Chiapinotto Spiazzi1, Andreas Sebastian Loureiro Mendez2, Fábio Gallas Leivas1, Daniela dos Santos Brum1, Francielli Weber Santos Cibin1
1Laboratório de Biotecnologia da Reprodução (Biotech), Campus Uruguaiana, Universidade Federal do Pampa, 97500-970 Uruguaiana, RS, Brazil
2Faculdade de Farmácia, Universidade Federal do Rio Grande do Sul, Av. Ipiranga 2752, 90610-000, Porto Alegre, RS, Brazil

Tóm tắt

Tribulus terrestris (TT) has been considered as a potential stimulator of testosterone production, which has been related with steroidal saponins prevailing in this plant. Cyclophosphamide (CP) is the most commonly used anticancer and immunosuppressant drug, which causes several toxic effects, especially on the reproductive system. Patients who need to use CP therapy exhibit reduced fertility or infertility, which impacts both physically and emotionally on the decision to use this drug, especially among young men. We hypothesized that the treatment with TT dry extract would protect the male reproductive system against CP toxicity. Mice received dry extract of TT (11 mg/kg) or vehicle by gavage for 14 days. Saline or CP was injected intraperitoneally at a single dose (100 mg/kg) on the 14th day. Animals were euthanized 24 h after CP administration, and testes and epididymis were removed for biochemical and histopathological analysis and sperm evaluation. The dry extract of TT was evaluated by HPLC analysis and demonstrated the presence of protodioscin (1.48%, w/w). CP exposure increased lipid peroxidation, reactive species, and protein carbonylation and altered antioxidant enzymes (SOD, CAT, GPx, GST, and GR). Moreover, acute exposure to CP caused a reduction on 17 β‐HSD activity, which may be related to the reduction in serum testosterone levels, histopathological changes observed in the testes, and the quality of the semen. The present study highlighted the role of TT dry extract to ameliorate the alterations induced by CP administration in mice testes, probably due to the presence of protodioscin.

Từ khóa


Tài liệu tham khảo

Dollery C. T., 1999, Therapeutic Drugs

10.1016/j.tox.2008.03.015

10.1016/0027-5107(75)90295-X

10.1002/1097-0142(19900501)65:9<1903::AID-CNCR2820650905>3.0.CO;2-#

10.1007/s12013-014-0376-8

10.2164/jandrol.108.005132

10.1016/j.fct.2010.05.067

10.1016/j.cca.2005.11.034

Al-Rawi A., 1985, Flora Of Kuwait

Akram M., 2011, Tribulus terrestris Linn.: a review article, Journal of Medicinal Plants Research, 5, 3601

10.1089/10755530360623374

10.1038/sj.ijir.3900265

10.1016/j.phymed.2007.11.011

10.4103/0253-7613.84974

10.1196/annals.1372.005

10.3923/ijp.2008.1.10

10.1016/j.jep.2009.09.031

10.1016/j.cbi.2009.03.017

10.1016/j.fitote.2012.01.006

10.1016/j.tox.2006.01.027

10.1016/j.toxrep.2014.12.016

10.1016/j.phytochem.2007.07.003

10.1016/0003-2697(79)90738-3

10.1074/jbc.M304854200

10.1016/0076-6879(90)86141-H

10.1016/S0021-9258(19)45228-9

10.1016/S0076-6879(84)05016-3

Paglia D. E., 1967, Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase, The Journal of Laboratory and Clinical Medicine, 70, 158

10.1016/S0076-6879(85)13062-4

10.1016/S0021-9258(19)42083-8

10.1016/0300-483X(85)90008-3

10.1016/S0021-9258(18)93926-8

10.1530/jrf.0.0880343

Geller M., 1976, The histopathology of acute nitrofurantoin-associated penumonitis, Annals of Allergy, 37, 275

10.1016/0003-2697(76)90527-3

Yu L. J., 1999, In vivo modulation of alternative pathways of P-450-catalyzed cyclophosphamide metabolism: impact on pharmacokinetics and antitumor activity, The Journal of Pharmacology and Experimental Therapeutics, 288, 928

10.1007/BF02912918

Boonsorn T., 2010, Effects of catechin addition to extender on sperm quality and lipid peroxidation in boar semen, American-Eurasian Journal of Agricultural and Environmental Science, 7, 283

10.1016/j.cbi.2014.11.001

10.1016/S0024-3205(02)01858-1

10.1007/s11101-005-2833-x

Thông tin
Thông tin xuất bản

Oxidative Medicine and Cellular Longevity

Tập 2018 Số 1

Thông tin tác giả