<i>Propionibacterium acnes</i> and <i>Staphylococcus epidermidis</i> Isolated from Refractory Endodontic Lesions Are Opportunistic Pathogens

Journal of Clinical Microbiology - Tập 48 Số 11 - Trang 3859-3869 - 2010
Sadia Niazi1, Douglas J. Clarke1, Thuy Do1, Steven C. Gilbert1, Francesco Mannocci1, D. Beighton1
1Dental Institute, King's College London, London, England

Tóm tắt

ABSTRACT The predominant cultivable microbiota from 20 refractory endodontic lesions (9 with abscesses and 11 without abscesses) were determined, and Propionibacterium acnes and Staphylococcus epidermidis were among the most predominant organisms. The number of species identified from lesions with abscesses (14.1 ± 2.6) was significantly greater ( P < 0.001) than the number from lesions without abscesses (7.4 ± 5.9). Comparison of perioral isolates using repetitive extragenic palindromic PCR of the same species from the same subjects demonstrated that the endodontic and skin populations were significantly different. The P. acnes isolates were typed on the basis of recA gene sequence comparison, and only three types (types I, II, and III) were identified among 125 isolates examined. However, we found that type I (type IA and IB) isolates were primarily isolated from the skin, while types II and III were significantly more likely to be isolated from the endodontic lesions ( P < 10 −10 ). We found that the robustness of the recA phylotypes was not strong by comparing the partial gene sequences of six putative virulence determinants, PAmce, PAp60, PA-25957, PA-5541, PA-21293, and PA-4687. The resulting neighbor-joining trees were incongruent, and significant (phi test; P = 2.2 × 10 −7 ) evidence of recombination was demonstrated, with significant phylogenetic heterogeneity being apparent within the clusters. P. acnes and S. epidermidis isolated from refractory endodontic infections, with or without periapical abscesses, are likely to be nosocomial infections.

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Journal of Clinical Microbiology

Tập 48 Số 11

3859-3869

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