Vitaliano Cama1,2, Caryn Bern2, Irshad M. Sulaiman1,2, Robert H. Gilman3, Eduardo Ticona4, Aldo Vivar5, Vivian Kawai6, Daniel Vargas6, Ling Zhou1,2, Lihua Xiao2
1Atlanta Research and Education Foundation, Atlanta, Georgia
2Division of Parasitic Diseases, National Center for Infectious Diseases, Centers, fur Disease Control and Prevention. Atlanta, Georgia
3Johns Hopkins University, Baltimore, Maryland
4Huspital Dos de Mayo, Lima, Peru
5Hospital Loayza, Lima, Peru
6Asociación Benéfica PRISMA, Lima, Peru
Tóm tắt
ABSTRACT: Cryptosporidium parasites from a cross‐sectional study conducted in two national hospitals in Lima, Peru were genetically characterized to deteimine the diversity of Cryptosporidium spp. in HIV‐positive people. A total of 2,672 patients participated in this study and provided 13,937 specimens. Cryptosporidium oocysts were detected by microscopy in 354 (13.3%) of the patients. Analysis of 951 Cryptosporidium‐ positive specimens from 300 patients using a small subunit rRNA‐based PCR‐RFLP tool identified 6 genotypes; Cryptosporidium hominis was the species most frequently detected (67.5%), followed by C. meleagridis (12.6%) and C. parvum (11.3%). Cryptosporidium canis (4.0%), C. felis (3.3%), and Cryptosporidium pig genotype (0.5%) were also found. These findings indicate that C. hominis is the predominant species in Peruvian HIV‐positive persons, and that zoonotic Cryptosporidium spp. account for about 30% of cryptosporidiosis in these patients.
