Cryptosporidium hominis n. sp. (Apicomplexa: Cryptosporidiidae) from Homo sapiens

Journal of Eukaryotic Microbiology - Tập 49 Số 6 - Trang 433-440 - 2002
Una Ryan1, Abbie Fall2, Lucy Ward3, Nawal Hijjawi2, Irshad M. Sulaiman4, Ronald D. Payer5, Richard C. Thompson2, Merle S. Olson6, Altaf A. Lal4, Lihua Xiao4
1Division of Veterinary and Biomedical Sciences, Murdoch University, Murdoch, Western Australia, 6150
2aDivision of Veterinary and Biomedical Sciences, Murdoch University, Murdoch, Western Australia 6150
3bFood Animal Health Research Program and Department of Veterinary Preventative Medicine, Ohio Agricu
4cDivision of Parasitic Diseases, National Center for Infectious Diseases, Centers for Disease Contro
5dU. S. Department of Agriculture, Animal Waste Pathogen Laboratory, Beltsville, Maryland 20705
6eUniversity of Calgary, Faculty of Medicine, Animal Resources Center, Department of Gastrointestinal

Tóm tắt

ABSTRACT. The structure and infectivity of the oocysts of a new species of Cryptosporidium from the feces of humans are described. Oocysts are structurally indistinguishable from those of Cryptosporidium parvum. Oocysts of the new species are passed fully sporulated, lack sporocysts, and measure 4.4–5.4 μm (mean = 4.86) × 4.4–5.9 μm (mean = 5.2μm) with a length to width ratio 1.0–1.09 (mean 1.07) (n = 100). Oocysts were not infectious for ARC Swiss mice, nude mice, Wistar rat pups, puppies, kittens or calves, but were infectious to neonatal gnotobiotic pigs. Pathogenicity studies in the gnotobiotic pig model revealed significant differences in parasite‐associated lesion distribution (P = 0.005 to P = 0.02) and intensity of infection (P = 0.04) between C. parvitm and this newly described species from humans. In vitro cultivation studies have also revealed growth differences between the two species. Multi‐locus analysis of numerous unlinked loci, including a preliminary sequence scan of the entire genome demonstrated this species to be distinct from C. parvum and also demonstrated a lack of recombination, providing further support for its species status. Based on biological and molecular data, this Cryptosporidium infecting the intestine of humans is proposed to be a new species Ciyptosporidium hominis n. sp.

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Journal of Eukaryotic Microbiology

Tập 49 Số 6

433-440

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