<i>Conus ventricosus </i>venom peptides profiling by HPLC‐MS: A new insight in the intraspecific variation

Journal of Separation Science - Tập 31 Số 3 - Trang 488-498 - 2008
Cristina Romeo1,2, Laura Di Francesco2, Marco Oliverio3, Paola Palazzo4, Gabriella Raybaudi Massilia4, Paolo Ascenzi4, Fabio Polticelli4, Maria Eugenia Schininà2
1Consorzio Interuniversitario "Istituto Nazionale Biostrutture e Biosistemi", Roma, Italy
2Dipartimento di Scienze Biochimiche, Università La Sapienza, Roma, Italy
3Dipartimento di Biologia Animale e dell’Uomo, Università La Sapienza, Roma, Italy
4Dipartimento di Biologia, Università “ROMA TRE”, Roma, Italy

Tóm tắt

AbstractConus is a genus of predatory marine gastropods that poison the prey with a complex mixture of compounds active on muscle and nerve cells. An individual cone snail's venom contains a mixture of pharmacological agents, mostly short, structurally constrained peptides. This study is focused on the composition of the venom employed by Conus ventricosus Gmelin, 1791, a worm‐hunting cone snail living in the Mediterranean Sea. For this purpose, LC coupled to MS techniques has been successfully used to establish qualitative and quantitative differences in conopeptides from minute amounts of venom ducts. We were able to prove variability in the venom conopeptide complement, possibly related to different trophic habits of the species in the Mediterranean Sea. Moreover, the information‐rich MS techniques enabled us to identify two novel C. ventricosus peptides, here named Conotoxin‐Vn and α‐Conotoxin‐Vn. On the basis of the structural data collected so far, we suggest that Conotoxin‐Vn is a conopeptide belonging to the ω‐family that recognizes calcium channels through a specific pharmacophore. Similarly, molecular modeling data suggest that α‐Conotoxin‐Vn should represent a competitive antagonist of neuronal nicotinic acetylcholine receptors (nAChRs).

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Journal of Separation Science

Tập 31 Số 3

488-498

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