Lei Zhang1, Shengnan Jin1, Chao Feng1, Haichao Song1, Sayed Haidar Abbas Raza2, Huabo Yu1, Liang Zhang1, Teng Chi1, Yanling Qi1, Dong-Xing Zhang1, Qian Ai-dong1, Ning Liu3, Xiaofeng Shan1
1Key Laboratory of Animal Production and Product Quality Safety of Ministry of Education, College of Animal Science and Technology, Jilin Agricultural University, Changchun, China
2College of Animal Science and Technology Northwest A&F University Yangling China
3Department of Veterinary Medicine, China Agriculture University, Beijing, China
Tóm tắt
AbstractAeromonas veronii (A. veronii) is an opportunistic pathogen of fish–human–livestock, which poses a threat to the development of aquaculture. Based on our previous studies on proteomics and genomics, we found out that the aodp gene may be related to the virulence of A. veronii TH0426. However, aodp gene encodes a hypothetical protein with an unknown function, and its role in A. veronii TH0426 is not clear. Here, we first constructed a mutant strain (△‐aodp) to investigate the functional role of aodp in A. veronii TH0426. Compared with the wild strain A. veronii TH0426, the growth rate of strain △‐aodp was slower and was resistant to neomycin and kanamycin, but sensitive to cephalexin. The swimming and swarming ability of △‐aodp strain decreased, and the pathogenicity to mice decreased by 15.84‐fold. Besides, the activity of caspase‐3 in EPCs infected with △‐aodp strain was 1.49‐fold lower than that of the wild strain. We examined 20 factors closely related to A. veronii virulence, among them 17 genes were down‐regulated as a result of aodp deficiency. This study laid a foundation for further studies on the pathogenesis of A. veronii.
