“Silent” Metaplasticity of the Late Phase of Long-Term Potentiation Requires Protein Phosphatases

Learning and Memory - Tập 9 Số 4 - Trang 202-213 - 2002
Newton H. Woo1, Peter Nguyen2,3
1Department of Physiology, University of Alberta School of Medicine, Edmonton, Alberta T6G 2H7, Canada
2Centre for Neuroscience
3University of Alberta School of Medicine, Edmonton, Alberta, T6G 2H7, Canada

Tóm tắt

The late phase of long-term potentiation (L-LTP) is correlated with some types of long-term memory, but the mechanisms by which L-LTP is modulated by prior synaptic activity are undefined. Activation of protein phosphatases by low-frequency stimulation (LFS) given before induction of L-LTP may significantly modify L-LTP. Using cellular electrophysiological recording methods in mouse hippocampal slices, we show that LFS given before induction of L-LTP inhibited L-LTP in an activity-dependent manner without affecting either basal synaptic strength or the early phase of LTP (E-LTP). This anterograde inhibitory effect of LFS was persistent, required N-methyl-D-aspartate (NMDA) receptor activation, and was blocked by inhibitors of protein phosphatase 1 (PP1) and protein phosphatase 2A (PP2A). These data indicate that certain patterns of LFS can activate PP1 and/or PP2A, and that long-lasting activation of these phosphatases by prior LFS can suppress the subsequent expression of L-LTP without affecting E-LTP. Because this inhibition of L-LTP is caused by prior synaptic activity that, alone, produced no net effect on synaptic efficacy, we suggest that this is a “silent” form of metaplasticity that may influence long-term information storage by modulating the capacity of synapses to express L-LTP after repeated bouts of activity.

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Learning and Memory

Tập 9 Số 4

202-213

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