β‐Alanine and taurine as endogenous agonists at glycine receptors in rat hippocampus in vitro

Journal of Physiology - Tập 539 Số 1 - Trang 191-200 - 2002
Masahiro Mori1, Beat H. Gähwiler1, Urs Gerber1
1Brain Research Institute, University of Zurich, CH-8057 Zurich, Switzerland

Tóm tắt

Electrophysiological and pharmacological properties of glycine receptors were characterized in hippocampal organotypic slice cultures. In the presence of ionotropic glutamate and GABAB receptor antagonists, pressure‐application of glycine onto CA3 pyramidal cells induced a current associated with increased chloride conductance, which was inhibited by strychnine. Similar chloride currents could also be induced with β‐alanine or taurine. Whole‐cell glycine responses were significantly greater in CA3 pyramidal cells than in CA1 pyramidal cells and dentate granule cells, while responses to GABA were similar among these three cell types. Although these results demonstrate the presence of functional glycine receptors in the hippocampus, no evidence for their activation during synaptic stimulation was found. Gabazine, a selective GABAA receptor antagonist, totally blocked evoked IPSCs in CA3 pyramidal cells. Glycine receptor activation is not dependent on transporter‐controlled levels of extracellular glycine, as no chloride current was observed in response to sarcosine, an inhibitor of glycine transporters. In contrast, application of guanidinoethanesulfonic acid, an uptake inhibitor of β‐alanine and taurine, induced strychnine‐sensitive chloride current in the presence of gabazine. These data indicate that modulation of transporters for the endogenous amino acids, β‐alanine and taurine, can regulate tonic activation of glycine receptors, which may function in maintenance of inhibitory tone in the hippocampus.

Từ khóa


Tài liệu tham khảo

10.1016/0166-2236(95)93923-L

10.1016/0306-4522(91)90421-J

10.1016/0306-4522(88)90263-1

10.1002/j.1460-2075.1988.tb03255.x

10.1016/0166-2236(91)90045-V

10.1111/j.1469-7793.1997.683bd.x

10.1113/jphysiol.1987.sp016493

Chattipakorn S. C., 2000, Activation of glycine‐gated chloride channels depresses excitatory and inhibitory transmission recorded in rat hippocampus, Society for Neuroscience Abstracts, 26, 93

10.1111/j.1469-7793.1998.463bt.x

10.1152/jn.2000.84.6.2715

10.1016/S0896-6273(00)80433-X

10.1002/(SICI)1098-1063(1996)6:4<347::AID-HIPO1>3.0.CO;2-I

10.1016/S0006-8993(98)00744-6

10.1016/S0166-2236(97)01122-3

Gähwiler B. H., 1998, Organotypic Slice Cultures of Neural Tissue, 461

Golding N. L., 1996, Context‐dependent synaptic action of glycinergic and GABAergic inputs in the dorsal cochlear nucleus, Journal of Neuroscience, 16, 2208, 10.1523/JNEUROSCI.16-07-02208.1996

10.1016/0169-328X(88)90002-2

10.1113/jphysiol.1991.sp018696

10.1126/science.281.5375.419

10.1523/JNEUROSCI.15-10-06890.1995

10.1113/jphysiol.1995.sp020739

10.1016/0304-3940(88)90519-8

10.1016/0959-4388(95)80044-1

10.1111/j.1432-1033.1990.tb19419.x

10.1016/0006-8993(90)90742-T

Liu Q. R., 1993, Molecular characterization of four pharmacologically distinct gamma‐aminobutyric acid transporters in mouse brain, Journal of Biological Chemistry, 268, 2106, 10.1016/S0021-9258(18)53968-5

10.1016/S0021-9258(18)41598-0

10.1073/pnas.89.24.12145

10.1002/j.1460-2075.1991.tb07779.x

10.1046/j.1471-4159.1998.71051785.x

10.1016/0306-4522(92)90151-Q

10.1002/j.1460-2075.1992.tb05529.x

Protti D. A., 1997, GABAergic and glycinergic IPSCs in ganglion cells of rat, Journal of Neuroscience, 17, 6075, 10.1523/JNEUROSCI.17-16-06075.1997

10.1097/00001756-199807130-00031

10.1016/S0306-4522(99)00384-X

10.1097/00001756-199501000-00019

10.1523/JNEUROSCI.20-10-03571.2000

10.1111/j.1471-4159.1993.tb09806.x

10.1016/0006-8993(91)90751-G

10.1152/jn.1998.80.5.2608

10.1016/0301-0082(91)90036-Z

10.1111/j.1469-7793.2000.00681.x

10.1016/0896-6273(92)90207-T

Smith K. E., 1992, Cloning and expression of a high affinity taurine transporter from rat brain, Molecular Pharmacology, 42, 563

10.1016/0306-4522(96)00046-2

10.1111/j.1476-5381.1989.tb11961.x

Pol A. N., 1988, Glycine and glycine receptor immunoreactivity in brain and spinal cord, Journal of Neuroscience, 8, 472, 10.1523/JNEUROSCI.08-02-00472.1988

10.1016/0306-4522(87)92968-X

Ye J. H., 1999, Inhibitory effect of ondansetron on glycine response of dissociated rat hippocampal neurons, Journal of Pharmacology and Experimental Therapeutics, 290, 104

10.1016/S0306-4522(98)00158-4

10.1111/j.1460-9568.1995.tb01125.x

Thông tin
Thông tin xuất bản

Journal of Physiology

Tập 539 Số 1

191-200

Thông tin tác giả