α-glucosidase and glycation inhibitory effects of costus speciosus leaves
Tóm tắt
Hyperglycaemia is a salient feature of poorly controlled diabetes mellitus. Rate of protein glycation is increased with hyperglycaemia leading to long term complications of diabetes. One approach of controlling blood glucose in diabetes targets at reducing the postprandial spikes of blood glucose. The objectives of this study were to assess the in vitro inhibitory effects of Costus speciosus (COS) leaves on α-amylase and α-glucosidase activities, fructosamine formation, protein glycation and glycation-induced protein cross-linking. Methanol extracts of COS leaves were used. Inhibitory effects on enzyme activities were measured using porcine pancreatic α-amylase and α-glucosidase from
in the presence of COS extract. Percentage inhibition of the enzymes and the IC50 values were determined. In vitro protein glycation inhibitory effect of COS leaves on early and late glycation products were measured using bovine serum albumin or chicken egg lysozyme with fructose. Nitroblue tetrazolium was used to assess the relative concentration of fructosamine and polyacrylamide gel electrophoresis was used to assess the degree of glycation and protein cross-linking in the reaction mixtures. α-Glucosidase inhibitory activity was detected in COS leaves with a IC50 of 67.5 μg/ml which was significantly lower than the IC50 value of Acarbose (p < 0.01). Amylase inhibitory effects occurred at a comparatively higher concentration of extract with a IC50 of 5.88 mg/ml which was significantly higher than the IC50 value of Acarbose (p < 0.01). COS (250 μg/ml) demonstrated inhibitory effects on fructosamine formation and glycation induced protein cross-linking which were in par with 1 mg/ml aminoguanidine were detected. Methanol extracts of COS leaves demonstrated in vitro inhibitory activities on α-glucosidase, fructosamine formation, glycation and glycation induced protein cross-linking. These findings provide scientific evidence to support the use of COS leaves for hypoglycemic effects with an added advantage in slowing down protein glycation.
Tài liệu tham khảo
International Diabetes Federation. IDF Diabetes Atlas. 6th ed. Brussels, Belgium: International Diabetes Federation; 2013. http://www.idf.org/diabetesatlas.
Meeprom A, Sompong W, Chan CB, Adisakwattana S. Isoferulic acid, a new anti-glycation agent, inhibits fructose-and glucose-mediated protein glycation in vitro. Molecules. 2013;18(6):6439–54.
Sadowska-Bartosz I, Bartosz G. Prevention of protein glycation by natural compounds. Molecules. 2015;20(2):3309–34.
Aronson D. Cross-linking of glycated collagen in the pathogenesis of arterial and myocardial stiffening of aging and diabetes. J Hypertens. 2003;21(1):3–12.
Goh SY, Cooper ME. The role of advanced glycation end products in progression and complications of diabetes. J Clin Endocrinol Metab. 2008;93(4):1143–52.
Singh VP, Bali A, Singh N, Jaggi AS. Advanced glycation end products and diabetic complications. Korean J Physiol Pharmacol. 2014;18(1):1–14.
Gkogkolou P, Böhm M. Advanced glycation end products: key players in skin aging? Dermato-Endocrinology. 2012;4(3):259–70.
Li J, Liu D, Sun L, Lu Y, Zhang Z. Advanced glycation end products and neurodegenerative diseases: mechanisms and perspective. J Neurol Sci. 2012;317(1):1–5.
Sheard NF, Clark NG, Brand-Miller JC, Franz MJ, Pi-Sunyer FX, Mayer-Davis E, et al. Dietary carbohydrate (Amount and Type) in the prevention and management of diabetes a statement by the American diabetes association. Diabetes Care. 2004;27(9):2266–71.
Mahomoodally MF, Subratty AH, Gurib-Fakim A, Choudhary MI, Nahar Khan S. Traditional medicinal herbs and food plants have the potential to inhibit key carbohydrate hydrolyzing enzymes in vitro and reduce postprandial blood glucose peaks in vivo. The Scientific World J. 2012; doi:10.1100/2012/285284.
Olaokun OO, McGaw LJ, Eloff JN, Naidoo V. Evaluation of the inhibition of carbohydrate hydrolysing enzymes, antioxidant activity and polyphenolic content of extracts of ten African Ficus species (Moraceae) used traditionally to treat diabetes. BMC Complementary and Alternative Medicine. 2013;13(1):94–103.
Sales PM, Souza PM, Simeoni LA, Magalhães PO, Silveira D. α-Amylase inhibitors: a review of raw material and isolated compounds from plant source. J Pharm Pharm Sci. 2012;15(1):141–83.
Kumar S, Narwal S, Kumar V, Prakash O. α-glucosidase inhibitors from plants: a natural approach to treat diabetes. Pharmacogn Rev. 2011;5(9):19–29.
Grover JK, Yadav S, Vitas V. Medicinal plants of India with antidiabetic potential. J Ethnopharmacol. 2002;81(1):81–100.
Ediriweera ERHSS, Ratnasooriya WD. A review on herbs used in treatment of diabetes mellitus by Sri Lankan ayurvedic and traditional physicians. Ayu. 2009;30(4):373–91.
Jung M, Park M, Lee CH, Kang Y, Kang ES, Kim SK. Antidiabetic agents from medicinal plants. Curr Med Chem. 2006;13:1203–18.
Modak M, Dixit P, Londhe J, Ghaskadbi S, Devasagayam TPA. Indian herbs and herbal drugs used for the treatment of diabetes. J Clin Biochem Nutr. 2007;40(3):163–73.
Rani AS, Sulakshana G, Patnaik S. Costus speciosus, An antidiabetic plant-review. Fons Scientia Journal of Pharmacy Research. 2012;1(3):52–3.
Pawar VA, Pawar PR. Costus speciosus: an important medicinal plant. International Journal of Science and Research. 2014;3(7):28–33.
Samarakoon KW, Lakmal HC, Kim SY, Jeon YJ. Electron spin resonance spectroscopic measurement of antioxidant activity of organic solvent extracts derived from the methanolic extracts of Sri Lankan thebu leaves (Costus speciosus). Journal of the National Science Foundation of Sri Lanka. 2014;42(3):209–16.
Subasinghe HWAS, Hettihewa LM, Gunawardena S, Liyanage T. Methanol and water extracts of Costus speciosus (j.könig) sm. leaves reverse the high-fat-diet induced peripheral insulin resistance in experimental Wistar rats. International Research Journal of Pharmacy. 2014;5(2):44–9.
Subasinghe HWAS, Hettihewa LM, Gunawardena S, Liyanage T. Evaluation of aqueous extract of Costus speciosus(J.König)Sm.leaf for hepatic and renal toxicities: biochemical and histopathological perspectives. European Journal of Pharmaceutical and Medical Research. 2015;2(4):1–12.
Girgis SM, Shoman TMT, Kassem SM, Ezz El-Din A, Abdel-Aziz KB. Potential Protective effect of Costus speciosus or its nanoparticles on streptozotocin-induced genotoxicity and histopathological alterations in rats. Journal of Nutrition & Food Sciences. 2015;S3:002. doi:10.4172/2155-9600.1000S3002.
Medagama AB, Bandara R, Abeysekera RA, Imbulpitiya B, Pushpakumari T. Use of complementary and alternative medicines (CAMs) among type 2 diabetes patients in Sri Lanka: a cross sectional survey. BMC Complementary and Alternative Medicine. 2014;14(1):374. doi:10.1186/1472-6882-14-374.
Vishalakshi DD, Asna U. Nutrient profile and antioxidant components of Costus Speciosus Sm, and Costus ignes Nak. Indian Journal of Natural Products and Resources. 2010;1:116–8.
Gavillán-Suárez J, Aguilar-Perez A, Rivera-Ortiz N, Rodríguez-Tirado K, Figueroa-Cuilan W, Morales-Santiago L, et al. Chemical profile and in vivo ypoglycemic effects of Syzygium jambos, Costus speciosus and Tapeinochilos ananassae plant extracts used as diabetes adjuvants in Puerto Rico. BMC Complementary and Alternative Medicine. 2015;15:244. doi:10.1186/s12906-015-0772-7.
Revathy J, Abdullah SS, Kumar PS. Antidiabetic effect of Costus Speciosus rhizome extract in alloxan induced albino rats. Journal of Chemistry and Biochemistry. 2014;2(1):13–22.
Ali HA, Almaghrabi OA, Afifi ME. Molecular mechanisms of anti-hyperglycemic effects of Costus speciosus extract in streptozotocin-induced diabetic rats. Saudi Medical Journal. 2014;35(12):1501–6.
Bavarva JH, Narasimhacharya AVRL. Antihyperglycemic and hypolipidemic effects of Costus speciosus in alloxan induced diabetic rats. Phytother Res. 2008;22(5):620–6.
Rajesh MS, Harish MS, Sathyaprakash RJ, Shetty AR, Shivananda TN. “Antihyperglycemic activity of the various extracts of Costus speciosus rhizomes”. Jof Natural Remedies. 2009;9(2):235–41.
Poongunran J, Perera HKI, Fernando WIT, Jayasinghe L, Sivakanesan R. α-Glucosidase and α-amylase inhibitory activities of nine Sri Lankan antidiabetic plants. British J Pharmaceutical Res. 2015;7(5):365–74.
Geethalakshmi R, Sarada DVL, Marimuthu P, Ramasamy K. α-Amylase inhibitory activity of Trianthema decandra L. Int J Biotechnol Biochemistry. 2010;6(3):369–76.
Bernfeld P. Amylases alpha and beta, in Methods in enzymlogy, Volume 1 (Academic Press, New York). Methods Enzymol. 1955;1:149–58.
Elya B, Basah K, Munim A, Yuliastuti W, Bangun A, Septiana EK. Screening of α-glucosidase inhibitory activity from some plants of Apocynaceae, Clusiaceae, Euphorbiaceae, and Rubiaceae. Journal of Biomedicine and Biotechnology. 2012; doi:10.1155/2012/281078.
Wijetunge DCR, Perera HKI. A novel in vitro method to detect inhibitors of protein glycation. Asian Journal of Medical Sciences. 2014;5(3):15–21.
Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970;227(5259):680–5.
Perera HKI, Ranasinghe HASK. A simple method to detect plant based inhibitors of glycation induced protein cross-linking. Asian Journal of Medical Sciences. 2015;6(1):28–33.
Dasgupta B, Pandey VB. A new Indian source of diosgenin (Costus speciosus). Experientia. 1970;26(5):475–6.
Srivastava S, Singh P, Mishra G, Jha KK, Khosa RL. Costus speciosus (Keukand): a review. Der Pharmacia Sinica. 2011;2(1):118–28.
Naidu PB, Ponmurugan P, Begum MS, Mohan K, Meriga B, RavindarNaik R, et al. Diosgenin reorganises hyperglycaemia and distorted tissue lipid profile in high‐fat diet-streptozotocin‐induced diabetic rats. J Sci Food Agric. 2015;95(15):3177–82.
Eliza J, Daisy P, Ignacimuthu S, Duraipandiyan V. Antidiabetic and antilipidemic effect of eremanthin from Costus speciosus (Koen.) Sm., in STZ-induced diabetic rats. Chem Biol Interact. 2009;182(1):67–72.
Eliza J, Daisy P, Ignacimuthu S, Duraipandiyan V. Normoglycemic and hypolipidemic effect of costunolide isolated from Costus speciosus (Koen ex. Retz.) Sm. in streptozotocin-induced diabetic rats. Chem Biol Interact. 2009;79(2):329–34.
Mosihuzzaman M, Nahar N, Ali L, Rokeya B, Khan AK, Nur EAM, et al. Hypoglycemic effects of three plants from eastern Himalayan belt. Diabetes Research. 1994;26(3):127–38.
Jothivel NPS, Appachi M, Singaravel S, Rasilingam D, Deivasigamani K, Thangavel S. Anti-diabetic activity of methanol leaf extract of Costus pictus D. Don in alloxan-induced diabetic rats. Journal of Health Sciences. 2007;53(6):655–63.
Devi VD, Asna U. Possible Hypoglycemic Attributes of Morus indica 1. and Costus speciosus: An in vitro Study. Malaysian Journal of Nutrition. 2015;21(1):83–91.
Dearlove RP, Greenspan P, Hartle DK, Swanson RB, Hargrove JL. Inhibition of protein glycation by extracts of culinary herbs and spices. J Med Food. 2008;11(2):275–81.
Eliza J, Daisy P, Ignacimuthu S. Antioxidant activity of costunolide and eremanthin isolated from Costus speciosus (Koen ex. Retz) Sm. Chem Biol Interact. 2010;188(3):467–72.
Majumdar M, Parihar PS. Antibacterial, anti-oxidant and antiglycatbion potential of Costus pictus from southern region, India. Asian J Plant Sci Res. 2012;2(2):95–101.
Nair SV, Hettihewa M, Rupasinghe HP. Apoptotic and inhibitory effects on cell proliferation of hepatocellular carcinoma HepG2 cells by methanol leaf extract of Costus speciosus. BioMed Research International. 2014; doi:10.1155/2014/637098.