Vietnam Journal of Biotechnology
2815-5912
Cơ quản chủ quản: N/A
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Các bài báo tiêu biểu
Diversity of endophytic fungi from medicinal plants Dysosma difformis (Hemsl & E.H. Wilson) T.H. Wang collected in Ha Giang and Lai Chau
Tập 21 Số 2 - Trang 365-373 - 2023
Plant endophytes are an interesting group of microorganisms that colonize the internal tissues of living plants and do not cause any disease symptoms in the host plant. It exists in different parts of plants, such as roots, leaves, and stems, and significantly affects the formation of metabolic products in plants by promoting the accumulation of important secondary metabolites. The present study f......
Cây thuốc lá chuyển gen mang cấu trúc RNAi kháng đồng thời hai loại virus gây bệnh khảm
- 2011
Tobacco mosaic virus (TMV) and cucumber mosaic virus (CMV) is one of the most important viral pathogens capable of affecting to many plant species, such as tomato, potato, tobacco, peppe etc. In order to generate fransgenic tobacco plants resistant to both TMV and CMV, RNA interference (RNAi) technology hasbeen applied in this study. A multi-fragment of 625 bp - CPi (including ......
#Cucumber mosaic virus #Nicotiana tabacum #RNAi #Tobacco mosaic virus #transgenic plant
Expression of soluble recombinant human SUMO protease’s active region fused with thioredoxin in Escherichia coli
Tập 22 Số 1 - Trang 187-196 - 2024
The human’s SUMO protease is commonly used in gene expression studies. However, it is challenging to extract this enzyme from its natural host due to degradation during extraction and high cost. Recombinant expression using Escherichia coli is considered for its high production from the fast growth rate and feasible manipulation. In this study, we aimed to express the human SUMO protease’s active ......
#thioredoxin #SUMO protease #recombinant expression #E. coli
Strategies to enhanced production of biomass and natural antioxidants of Cichorium intybus L. hairy roots by using nanoparticle elicitors
Tập 22 Số 2 - Trang 305-317 - 2024
This study describes the influence of silver nanoparticles (AgNPs) and selenium nanoparticles (SeNPs) on the biomass and phytochemical production in the hairy root (HR) cultures of Cichorium intybus L. The HRs were grown in ½ Murashige and Skoog (MS) medium supplemented with sucrose (30 g/L) and AgNPs or SeNPs at two selected concentrations for 25 days on the shaker at 110 rpm in the dark. The res......
#antioxidant activity #Cichorium intybus L. #flavonoids #growth curve #hairy root #phenolic
Mitochondrial DNA insertions into nuclear genome (numts) in Vietnam sheep: Implications and caveats for the utility of the mitochondrial DNA markers in genetic diversity studies in sheep
Tập 21 Số 4 - Trang 655-667 - 2023
Nuclear insertions of mitochondrial DNA sequences (numts) can introduce errors in population genetic studies using mitochondrial DNA markers (mtDNA markers). Despite their prevalence in mammalian genomes and the potential risk of contamination with mtDNA markers, adequate precautions are often overlooked. When investigating the genetic diversity of Vietnam sheep, we found numt contamination in D-l......
Optimization of biosurfactant production for enhanced crude-oil degradation by a Candida tropicalis 1214-BK14 isolated from white tiger oilfiel - Vung Tau, Vietnam
Tập 15 Số 1 - Trang 189-196 - 2017
Biosurfactants are amphiphilic molecules with effective surface-active and biological properties applicable to replace synthetic surfactant in petroleum industry. Interest in microbial surfactants has been steadily increasing in recent years, as they have numerous advantages compared to chemical surfactants including a lower toxicity, better environmental compatibility and effective properties at ......
#Biosurfactant #yeast #crude-oil #response surface methodology
KHẢO SÁT HIỆU ỨNG GÂY TỬ VONG CỦA AZADIRACHTIN LÊN TẾ BÀO ẤU TRÙNG NGÀI GẠO (CORCYRA CEPHALONICA ST.) NUÔI CẤY IN VITRO
- 2012
Việc lạm dụng thuốc trừ sâu tổng hợp đã dẫn đến những hậu quả nghiêm trọng như sự kháng thuốc, bùng nổ dịch bệnh và nhiều tác hại khác đối với thiên địch và môi trường, từ đó đã thúc đẩy nhu cầu nghiên cứu thuốc trừ sâu thiên nhiên với hiệu quả phòng trị và độ an toàn sinh học cao. Vì vậy, việc sử dụng hoạt chất sinh học gốc thảo mộc từ lâu đã được khuyến cáo ở nhiều nước. Trong ......
#Azadirachtin #cultured cells #mortality effect #rice moth (Corcyra cephalonica St.) #trypan blue
The pathogenicity and reproduction capability of five epn strains on white grubs (Alissonotum impressicolle Arrow) in the laboratory conditions
Tập 15 Số 2 - Trang 277-284 - 2017
Five entomopathogenic nematode strains, included 3 strains of the genus Steinernema, e.g. S-PQ16, SS-CP12 and S-TX1, and two strains of genus Heterorhabditis indica, e.g. H-CB3452 and H-KT3987 were evaluated on virulence and reproduction capacity on white grubs of black scarab (Alissonotum impressicolle Arrow) a serious pest damaging in the soils of sugarcane and many economic crops in Western Hig......
#Alissonotum impressicolle #indigenous EPN nematodes #S-CP12 #S-TX1 #S-PQ16 #H-KT3987 #H-CB3452 #LC50 #pathogencity #reproduction.
Knock out aryl hydrocarbon receptor gene in adipose-derived mesenchymal stem cell line (PT-5006) by CRISPR/Cas9
Tập 22 Số 1 - Trang 69-77 - 2024
Mesenchymal stem cells (MSCs) have garnered significant attention in biomedical studies due to their remarkable properties, such as self-renewal, differentiation into diverse cell types and immune responses. The proliferation and differentiation of MSCs are significantly influenced by the ligand-dependent transcription factor known as the aryl hydrocarbon receptor (AhR). In order to understand the......
#Adipose-derived MSCs #aryl hydrocarbon receptor #knockout gene #CRISPR/Cas9 #puromycin selection
Modifying the hemagglutinin gene with the packaging signals to improve the ability of replication in eggs of IBT-RG02 vaccine virus strain
Tập 21 Số 4 - Trang 637-643 - 2023
The hemagglutinin (HA) and neuraminidase (NA) genes were derived from the popular H5N1 virus and the remaining six internal segments were derived from the A/Puerto Rico/8/34 strain (H1N1, PR8). However, some of these candidate strains have been reported to produce relatively low yields in vaccine manufacture does not replicate well in chicken eggs, posing an obstacle to egg-based vaccine productio......