STEROIDS is an international research journal devoted to studies on all chemical and biological aspects of steroidal moieties. The journal focuses on both experimental and theoretical studies on the biology, chemistry, biosynthesis, metabolism, molecular biology, physiology and pharmacology of steroids and other molecules that target or regulate steroid receptors. Manuscripts presenting clinical research related to steroids, steroid drug development, comparative endocrinology of steroid hormones, investigations on the mechanism of steroid action and steroid chemistry are all appropriate for submission for peer review. STEROIDS publishes both original research and timely reviews. For details concerning the preparation of manuscripts see Instructions to Authors, which is published in each issue of the journal.
Sundell Ann-Christine, Mikola Heikki, Hänninen Elina
O-Carboxymethyl-, O-(4-aminobutyl)-, and O-(6-aminohexyl)-oximes of 6-oxoestradiol and testosterone were labeled using a described general labeling method with a europium chelate. A stable amide or thiourea bond was formed between the europium label and carboxyl or amino derivative of the steroid, respectively. These labeled “tracers” were purified and briefly tested in a competitive time-resolved fluoroimmunoassay using monoclonal antibodies raised against O-(carboxymethyloxime)-bovine serum albumin derivatives of estradiol and testosterone. Only buffer-based standards were used in a model system for the testing. These studies indicate only minor functional differences while using these antibodies due to the length of the spacer arm, and a higher sensitivity in the clinically relevant standard range was reached with the longer spacers.