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Den Gefrierpunkt verschiedener Mischungen von Alkohol und Wasser
Springer Science and Business Media LLC - Tập 20 - Trang 249-249
F. M. Raoult
#Analytical Chemistry #Biochemistry #general #Laboratory Medicine #Characterization and Evaluation of Materials #Food Science #Monitoring/Environmental Analysis
Die quantitative Bestimmung von Albuminen im Blutserum
Springer Science and Business Media LLC - Tập 213 - Trang 312-313 - 1965
P. Haas
New type of matrix for matrix-assisted laser desorption mass spectrometry of polysaccharides and proteins
Springer Science and Business Media LLC - Tập 349 - Trang 473-474 - 1994
Jürgen O. Metzger, Ralf Woisch, Wilfried Tuszynski, Raimond Angermann
3-Aminoquinoline is introduced as matrix for matrix-assisted laser desorption mass spectrometry of polysaccharides and proteins. The first MALD mass spectrum of the polysaccharide inulin is reported. 3-Aminoquinoline seems to be more effective than the commonly used acidic matrix 2,5-dihydroxybenzoic acid, assisting in the desorption and ionisation of inulin. A higher sensitivity, sharper peaks and a lower background were observed. 3-Aminoquinoline is also quite effective when measuring the protein myoglobin.
Surface plasmon resonance sensing of Ebola virus: a biological threat
Springer Science and Business Media LLC - Tập 412 - Trang 4101-4112 - 2020
Pushpendra K. Sharma, Jyoti S. Kumar, Virendra V. Singh, Utpal Biswas, Shyam S. Sarkar, Syed I. Alam, Paban K. Dash, Mannan Boopathi, Kumaran Ganesan, Rajeev Jain
Here, different monoclonal antibodies (mAb1, mAb2 and mAb3) of Ebola virus were screened in a real-time and label-free manner using surface plasmon resonance (SPR) to select an appropriate antibody for biosensor applications against a biological warfare agent. For this purpose, a gold SPR chip was modified with 4-mercaptobenzoic acid (4-MBA), and modification was confirmed by FTIR-ATR and EIS. The 4-MBA-modified gold SPR chip was used for immobilization of the recombinant nucleoprotein of Ebola (EBOV-rNP), and the interactions of mAb1, mAb2 and mAb3 were then investigated to determine the best mAb based on the affinity constant (KD), expressed as equilibrium dissociation constant. KD values of 809 nM, 350 pM and 52 pM were found for the interaction of mAb1, mAb2 and mAb3 of Ebola with the immobilized EBOV-rNP, respectively, thus reflecting the high affinity of mAb3. This was confirmed by ELISA results. The thermodynamic parameters (ΔG, ΔH and ΔS) for the interaction between mAb3 and EBOV-rNP were also determined, which revealed that the interaction was spontaneous, endothermic and driven by entropy. The SPR limit of detection of EBOV-rNP with mAb3 was 0.5 pg ml−1, showing mAb3 to be the best high-affinity antibody in our study. This study has opened up new possibilities for SPR screening of different monoclonal antibodies of BWA through the convergence of materials science and optical techniques.
Eine Brom-Thermoprobe zur Untersuchung der �le und Fette
Springer Science and Business Media LLC - Tập 54 Số 8 - Trang 430-431 - 1915
C. Stiepel
Das Winklersche Ammoniakmeßverfahren
Springer Science and Business Media LLC - Tập 213 - Trang 209-210 - 1965
H. Monien
Mikrotitration von Peptiden und Aminosäuren
Springer Science and Business Media LLC - Tập 305 - Trang 273-276 - 1981
H. P. Deppeler, G. Witthaus
Eine nichtwäßrige Titration von Peptiden und Aminosäuren mit Perchlorsäure unter spezieller Berücksichtigung von Verbindungen dieser Substanzklasse mit Thioether- und Sulfidfunktionen wird beschrieben. Störungen durch Bildung von Quecksilberkomplexen dieser Schwefelgruppen bei Zusatz von Quecksilber(II)-acetat werden durch das Lösungsmittel Nitromethan-Ameisensäure verhindert. Probenmengen im Mikromol-Bereich werden mit einer relativen Standardabweichung von unter 2% titriert.
über die Bestimmung von PhthalsÄure mit Hilfe der Atomabsorptionsspektrophotometrie
Springer Science and Business Media LLC - Tập 231 - Trang 385-385 - 1967
M. Matucha
Untersuchungen zur Verwendbarkeit der Differential-pulse-anodic-stripping-Voltammetrie für die Bestimmung der chemischen Species des Elements Zn in anthropogen belasteten Flußwässern
Springer Science and Business Media LLC - - 1978
K. Kritsotakis, H. J. Tobschall
Die Möglichkeit der Anwendung der DPASV mit der HMDE für die qualitative und quantitative Erfassung von Schwermetallen in anthropogen stark belasteten aquatischen Systemen wird an Hand der Zinkbestimmung untersucht. Es wird demonstriert, daß in solchen Wässern die Anwendung der DPASV nur unter bestimmten Voraussetzungen sinnvoll ist, weil sie durch die Gegenwart oberflächenaktiver Stoffe erheblich gestört wird. Durch Filtration der Wasserproben mit Filtern von wenigstens 0,2 μm Porendurchmesser werden diese Störungen weitgehend aufgehoben.
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