Structural features of DNA in competent Bacillus subtilisSpringer Science and Business Media LLC - Tập 113 - Trang 316-330 - 1971
W. J. Harris, G. C. Barr
For efficient transformation with B. subtilis, recipient cells must be grown to the state termed competence. Previous findings indicated that such competent cells contained DNA which exhibited about 5% single-strandedness. In this work, the physico-chemical properties of this DNA are compared to artificially “nicked” DNA. Evidence is presented that breakdown of the host DNA occurs during growth to...... hiện toàn bộ
Expression of resistance gene analogs in woodland strawberry (Fragaria vesca) during infection with Phytophthora cactorumSpringer Science and Business Media LLC - Tập 291 - Trang 1967-1978 - 2016
Xiao-Ren Chen, May Bente Brurberg, Abdelhameed Elameen, Sonja Sletner Klemsdal, Inger Martinussen
Important losses in strawberry production are often caused by the oomycete Phytophthora cactorum, the causal agent of crown rot. However, very limited studies at molecular levels exist of the mechanisms related to strawberry resistance against this pathogen. To begin to rectify this situation, a PCR-based approach (NBS profiling) was used to isolate strawberry resistance gene analogs (RGAs) with a...... hiện toàn bộ
Expression and functional analysis of genes encoding cytokinin receptor-like histidine kinase in maize (Zea mays L.)Springer Science and Business Media LLC - Tập 289 - Trang 501-512 - 2014
Bo Wang, Yanhong Chen, Baojian Guo, Muhammad Rezaul Kabir, Yingyin Yao, Huiru Peng, Chaojie Xie, Yirong Zhang, Qixin Sun, Zhongfu Ni
Cytokinin signaling is vital for plant growth and development which function via the two-component system (TCS). As one of the key component of TCS, transmembrane histidine kinases (HK) are encoded by a small gene family in plants. In this study, we focused on expression and functional analysis of cytokinin receptor-like HK genes (ZmHK) in maize. Firstly, bioinformatics analysis revealed that seve...... hiện toàn bộ
Two cold-inducible genes encoding lipid transfer protein LTP4 from barley show differential responses to bacterial pathogensSpringer Science and Business Media LLC - Tập 252 - Trang 162-168 - 1996
A. Molina, I. Diaz, P. Carbonero, F. García-Olmedo, I. K. Vasil
The barley genesHvLtp4.2 andHvLtp4.3 both encode the lipid transfer protein LTP4 and are less than 1 kb apart in tail-to-tail orientation. They differ in their non-coding regions from each other and from the gene corresponding to a previously reportedLtp4 cDNA (nowLtp4.1). Southern blot analysis indicated the existence of three or moreLtp4 genes per haploid genome and showed considerable polymorph...... hiện toàn bộ