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Springer Science and Business Media LLC - - 2000
Tissue distribution and immunohistochemical localization of the collagen-binding heat-shock protein gp46 in neonatal rats
Springer Science and Business Media LLC - Tập 28 - Trang 895-902 - 1996
Brian J. Pak, Judith B. Vanhorne, Stephen C. Pang
Collagen-binding heat-shock proteins ofM r 46–47 kDa have been postulated to function as putative molecular chaperones in the biosynthesis of collagen in several species. The rat homologue of this family of heat-shock proteins is called gp46. In the present study, we employed Western blotting and immunohistochemical methods to determine the tissue distribution and cellular localization of gp46 in ...... hiện toàn bộ
Autoradiography-based cytochemical detection of ecto-ATPase, ecto-ADPase, 5?-nucleotidase, and extracellular adenosine production, employing 141Ce3+ as a capturing agent
Springer Science and Business Media LLC - Tập 27 Số 7 - Trang 555-564 - 1995
Ognjen Čulić, Raf Lemmens, H. Teuchy, Luc Vanduffel
Combined analysis of in situ hybridization, cell cycle and structural markers using reflectance and immunofluorescence confocal microscopy
Springer Science and Business Media LLC - Tập 27 - Trang 15-23 - 1995
Gustavo Linares-Cruz, Guy Millot, Patricia De Cremoux, Janny Vassy, Birgitta Olofsson, Jean Paul Rigaut, Fabien Calvo
A method for the simultaneous detection of mRNA by reflectance in situ hybridization (RISH), cell cycle and structural markers by immunofluorescence using confocal laser scanning microscopy is presented. The mRNA expression of two ras-related genes rhoB and rhoC was analysed in human breast cancer cell lines and human histological specimens (breast cancer tissues and skin biopsies). In breast canc...... hiện toàn bộ
Fine structural localization of 3β-hydroxysteroid dehydrogenase in rat corpus luteum
Springer Science and Business Media LLC - Tập 5 - Trang 437-449 - 1973
Gönül Bara, Winston A. Anderson
A procedure for the ultracytochemical demonstration of 3β-hydroxysteroid dehydrogenase (3β-HSD) is described and the results for the localization of this enzyme in the lutein cells of the rat corpus luteum are presented. The procedure involved pre-fixation with aldehydes and incubation of small blocks. Short fixation (maximum 30 min) in 0.1% glutaraldehyde, 2% depolymerized paraformaldehyde or in ...... hiện toàn bộ
Problems encountered with BPST in dehydrogenase histochemistry
Springer Science and Business Media LLC - Tập 13 - Trang 338-338 - 1981
P. B. Gahan, A. L. Dawson
[3H]Thymidine autoradiographic and alkaline phosphatase histochemical studies of intestinal metaplasia of the human stomach
Springer Science and Business Media LLC - Tập 15 - Trang 953-959 - 1983
Masayoshi Hashimoto, Akira Tokunaga, Keigo Nishi, Masayo Wada, Koji Masumori, Yoshimasa Kumagae, Hideaki Numajiri, Norio Matsukura, Masayuki Yoshiyasu, Noritake Tanaka, Akiro Shirota, Goro Asano
The relationship between cell proliferation and enzyme activity in intestinal metaplasia of the human stomach was studied using a combined method of [3H]thymidine autoradiography and alkaline phosphatase histochemistry on the same section. Three types of intestinal metaplasia were observed depending on variations in both enzymatic activity and isotope labelling. One type shows alkaline phosphatase...... hiện toàn bộ
An investigation of new commercial samples of Methyl Green and Pyronin Y
Springer Science and Business Media LLC - Tập 19 - Trang 381-384 - 1987
H. Lyon, P. Jakobsen, P. Høyer, A. P. Andersen
New commercial samples of Methyl Green (Gurr Certistain), Pyronine G (Gurr Certistain) and Pyronin Y (Polysciences) have been investigated using spectrophotometry, thin layer chromatography and nuclear magnetic resonance, in addition to standardized simultaneous and sequential staining methods using purified Ethyl Green and pure Pyronin Y as reference dyes. The Methyl Green was found to be Ethyl G...... hiện toàn bộ
Identification of vascular endothelial cells in murine omentum using the lectin,Dolichos biflorus agglutinin: Possible applications in the study of angiogenesis
Springer Science and Business Media LLC - - 1989
R. Williams, David Robertson, A. J. S. Davies
Further evidence for the DNA base specificity of light-induced banding
Springer Science and Business Media LLC - Tập 16 Số 5 - Trang 521-528 - 1984
L. Ferruchhi, A. T. Sumner
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