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Springer Science and Business Media LLC - - 2000
Tissue distribution and immunohistochemical localization of the collagen-binding heat-shock protein gp46 in neonatal rats
Springer Science and Business Media LLC - Tập 28 - Trang 895-902 - 1996
Brian J. Pak, Judith B. Vanhorne, Stephen C. Pang
Collagen-binding heat-shock proteins ofM r 46–47 kDa have been postulated to function as putative molecular chaperones in the biosynthesis of collagen in several species. The rat homologue of this family of heat-shock proteins is called gp46. In the present study, we employed Western blotting and immunohistochemical methods to determine the tissue distribution and cellular localization of gp46 in ...... hiện toàn bộ
Combined analysis of in situ hybridization, cell cycle and structural markers using reflectance and immunofluorescence confocal microscopy
Springer Science and Business Media LLC - Tập 27 - Trang 15-23 - 1995
Gustavo Linares-Cruz, Guy Millot, Patricia De Cremoux, Janny Vassy, Birgitta Olofsson, Jean Paul Rigaut, Fabien Calvo
A method for the simultaneous detection of mRNA by reflectance in situ hybridization (RISH), cell cycle and structural markers by immunofluorescence using confocal laser scanning microscopy is presented. The mRNA expression of two ras-related genes rhoB and rhoC was analysed in human breast cancer cell lines and human histological specimens (breast cancer tissues and skin biopsies). In breast canc...... hiện toàn bộ
Fine structural localization of 3β-hydroxysteroid dehydrogenase in rat corpus luteum
Springer Science and Business Media LLC - Tập 5 - Trang 437-449 - 1973
Gönül Bara, Winston A. Anderson
A procedure for the ultracytochemical demonstration of 3β-hydroxysteroid dehydrogenase (3β-HSD) is described and the results for the localization of this enzyme in the lutein cells of the rat corpus luteum are presented. The procedure involved pre-fixation with aldehydes and incubation of small blocks. Short fixation (maximum 30 min) in 0.1% glutaraldehyde, 2% depolymerized paraformaldehyde or in ...... hiện toàn bộ
Problems encountered with BPST in dehydrogenase histochemistry
Springer Science and Business Media LLC - Tập 13 - Trang 338-338 - 1981
P. B. Gahan, A. L. Dawson
Identification of vascular endothelial cells in murine omentum using the lectin,Dolichos biflorus agglutinin: Possible applications in the study of angiogenesis
Springer Science and Business Media LLC - - 1989
R. Williams, David Robertson, A. J. S. Davies
Further evidence for the DNA base specificity of light-induced banding
Springer Science and Business Media LLC - Tập 16 Số 5 - Trang 521-528 - 1984
L. Ferruchhi, A. T. Sumner
Formation of crystalloid inclusions in the small intestine of neonatal pigs: an immunocytochemical study using colloidal gold
Springer Science and Business Media LLC - Tập 25 - Trang 19-29 - 1993
Lázló G. Kőműves, Buford L. Nichols, T. William Hutchens, Julian P. Heath
Enterocytes of the small intestine in 1-day-old suckling piglets contain numerous vesicles in the apical cytoplasm and a large granule located beneath the nucleus. Within the next 3 days, these granules transform into electron-dense crystalloid inclusions. These membrane-bound inclusions are up to 10 μm in length and 1–2 μm in diameter, and they are composed of electron-dense lamellae 3.9 nm apart...... hiện toàn bộ
Mechanism of connective tissue techniques I. The effect of dye concentration and staining time on anionic dye procedures
Springer Science and Business Media LLC - Tập 25 - Trang 821-829 - 1993
P. E. Reid, M. Iagallo, S. Nehr, M. Jankunis, L. Morrow, L. Trueman
Anionic dye connective tissue procedures were performed by staining for 5 min and 24 h with (a) 0.00018m and 0.0018m solutions of 28 dyes, and 0.018m solutions of 21 dyes in saturated picric acid (SPA), and (b) 0.0018m and 0.018m solutions of 20 dyes in 1% (w/v) phosphomolybdic acid (PMA). The staining obtained with dyes in SPA was classified as selective (no cytoplasmic staining), moderately sele...... hiện toàn bộ
Immunocytochemical localization of intracellular antigens with SEM
Springer Science and Business Media LLC - Tập 12 Số 4 - Trang 435-447 - 1980
Nakane, P. K., Hartman, A. L.
A method for the localization of intracellular antigens with a scanning electron microscope using peroxidase-labelling antibodies is described. A search for a hydrogen donor which may be deposited at the sites of antigen by enzymatic action and emit secondary electrons or generate backscatter electrons was made. It was found that when 4-chloro-1-naphthol was used, the peroxidase deposited reaction...... hiện toàn bộ
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