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Sulfolobus tokodaii ST2133 is characterized as a thioredoxin reductase-like ferredoxin:NADP+ oxidoreductase
Springer Science and Business Media LLC - - 2014
Zhen Yan, Young‐Woo Nam, Shinya Fushinobu, Takayoshi Wakagi
Desulfosporosinus acididurans sp. nov.: an acidophilic sulfate-reducing bacterium isolated from acidic sediments
Springer Science and Business Media LLC - Tập 19 - Trang 39-47 - 2014
Irene Sánchez-Andrea, Alfons J. M. Stams, Sabrina Hedrich, Ivan Ňancucheo, D. Barrie Johnson
Three strains of sulfate-reducing bacteria (M1T, D, and E) were isolated from acidic sediments (White river and Tinto river) and characterized phylogenetically and physiologically. All three strains were obligately anaerobic, mesophilic, spore-forming straight rods, stained Gram-negative and displayed variable motility during active growth. The pH range for growth was 3.8–7.0, with an optimum at p...... hiện toàn bộ
Thermostable and highly specific l-aspartate oxidase from Thermococcus litoralis DSM 5473: cloning, overexpression, and enzymological properties
Springer Science and Business Media LLC - Tập 22 - Trang 59-71 - 2017
Tsubasa Washio, Tadao Oikawa
We successfully expressed the l-aspartate oxidase homolog gene (accession no: OCC_06611) of Thermococcus litoralis DSM 5473 in the soluble fraction of Escherichia coli BL21 (DE3) using a pET21b vector with 6X His tag at its C-terminus. The gene product (Tl-LASPO) showed l-aspartate oxidase activity in the presence of FAD in vitro, and this report is the first that details an l-aspartate oxidase de...... hiện toàn bộ
Thích ứng nhiệt của phosphotransferase hygromycin B mesophilic qua tiến hóa hướng đích trong Archaea siêu nhiệt: lựa chọn một dấu hiệu di truyền ổn định cho việc chuyển gen vào Sulfolobus solfataricus Dịch bởi AI
Springer Science and Business Media LLC - Tập 5 - Trang 153-159 - 2001
Raffaele Cannio, Patrizia Contursi, Mosè Rossi, Simonetta Bartolucci
Một phiên bản đột biến của gen phosphotransferase hygromycin B (hph mut ) từ Escherichia coli, được tách lọc qua quá trình tiến hóa hướng đích ở 75°C trong các biến thể của một chủng Sulfolobus solfataricus nhiệt cực, đã được đặc trưng về độ ổn định di truyền cả trong chủng mesophilic nguyên bản và chủng nhiệt cực mới. Gen này được chứng m...... hiện toàn bộ
#phosphotransferase #hygromycin B #Sulfolobus solfataricus #di truyền ổn định #enzym nhiệt #
Lysis efficiency of standard DNA extraction methods for Halococcus spp. in an organic rich environment
Springer Science and Business Media LLC - Tập 12 - Trang 301-308 - 2007
S. Leuko, F. Goh, R. Ibáñez-Peral, B. P. Burns, M. R. Walter, B. A. Neilan
The extraction of nucleic acids from a given environment marks a crucial and essential starting point in any molecular investigation. Members of Halococcus spp. are known for their rigid cell walls, and are thus difficult to lyse and could potentially be overlooked in an environment. Furthermore, the lack of a suitable lysis method hinders subsequent molecular analysis. The effects of six differen...... hiện toàn bộ
The periplasmic space in Thermus thermophilus : evidence from a regulation-defective S-layer mutant overexpressing an alkaline phosphatase
Springer Science and Business Media LLC - - 2002
Pablo Castán, Olga Zafra, Renata Moreno, Miguel A. de Pedro, Cristina Vallés, Felipe Cava, Eddy Caro, Heinz Schwarz, José Berenguer
Comparative genomics reveals probable adaptations for xylose use in Thermoanaerobacterium saccharolyticum
Springer Science and Business Media LLC - Tập 28 - Trang 1-14 - 2024
Mateus Bernabe Fiamenghi, Juliana Silveira Prodonoff, Guilherme Borelli, Marcelo Falsarella Carazzolle, Gonçalo Amarante Guimaraes Pereira, Juliana José
Second-generation ethanol, a promising biofuel for reducing greenhouse gas emissions, faces challenges due to the inefficient metabolism of xylose, a pentose sugar. Overcoming this hurdle requires exploration of genes, pathways, and organisms capable of fermenting xylose. Thermoanaerobacterium saccharolyticum is an organism capable of naturally fermenting compounds of industrial interest, such as ...... hiện toàn bộ
Gene replacement in Haloarcula marismortui: construction of a strain with two of its three chromosomal rRNA operons deleted
Springer Science and Business Media LLC - Tập 9 - Trang 427-435 - 2005
Daqi Tu, Gregor Blaha, Peter B. Moore, Thomas A. Steitz
Site-directed mutagenesis were done in Haloarcula marismortui using the strategy that Khorana and coworkers devised for deleting the bacteriorhodopsin gene from Halobacterium halobium [Krebs et al. Proc Natl Acad Sci USA 90:1987–1991 (1993)]. Strains have been prepared from H. marsimortui, which normally has three rRNA operons, that are missing either its rrnB operon or both its rrnB and rrnC oper...... hiện toàn bộ
Sequencing of three lambda clones from the genome of alkaliphilic Bacillus sp. strain C-125
Springer Science and Business Media LLC - - 1999
Hideto Takami, Kaoru Nakasone, Nagahisa Ogasawara, Chie Hirama, Yuka Nakamura, Noriaki Masui, Fumie Fuji, Yoshihiro Takaki, Akihisa Inoue, Koki Horikoshi
Polyphasic analysis of Thermus isolates from geothermal areas in Iceland
Springer Science and Business Media LLC - Tập 10 - Trang 563-575 - 2006
Gudmundur O. Hreggvidsson, Sigurlaug Skirnisdottir, Bart Smit, Sigridur Hjorleifsdottir, Viggo Th. Marteinsson, Solveig Petursdottir, Jakob K. Kristjansson
Genetic relationships and diversity of 101 Thermus isolates from different geothermal regions in Iceland were investigated by using multilocus enzyme electrophoresis (MLEE) and small subunit ribosomal rRNA (SSU rRNA) sequence analysis. Ten polymorphic enzymes were used and seven distinct and genetically highly divergent lineages of Thermus were observed. Six of seven lineages could be assigned to ...... hiện toàn bộ
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