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Intragenomic diversity of the V1 regions of 16S rRNA genes in high-alkaline protease-producing Bacillus clausii spp
Springer Science and Business Media LLC - Tập 11 - Trang 597-603 - 2007
Yasushi Kageyama, Yoshihiro Takaki, Shigeru Shimamura, Shinro Nishi, Yuichi Nogi, Kohsuke Uchimura, Tohru Kobayashi, Jun Hitomi, Katsuya Ozaki, Shuji Kawai, Susumu Ito, Koki Horikoshi
Alkaliphilic Bacillus sp. strain KSM-K16, which produces high-alkaline M-protease, was characterized phenotypically, biochemically and genetically. This strain was identified as Bacillus clausii based on the results of taxonomic studies, including sequencing of the 16S rRNA gene and DNA-DNA hybridization. Seven rRNA operons in the genome were identified by pulsed-field gel electrophoresis. Sequenc...... hiện toàn bộ
A denitrifying bacterium from the deep sea at 11 000-m depth
Springer Science and Business Media LLC - Tập 1 - Trang 207-211 - 1997
H. Tamegai, Lina Li, Noriaki Masui, Chiaki Kato
The denitrifying bacterium strain MT-1 was isolated from the mud of the Mariana Trench. The optimal temperature and pressure for growth of this bacterium were found to be 30°C and 0.1 MPa, respectively. However, it showed greater tolerance to low temperature (4°C) and high hydrostatic pressure (50 MPa) as compared with denitrifiers obtained from land. From the results, it can be said that this or...... hiện toàn bộ
Trehalose production at high temperature exploiting an immobilized cell bioreactor
Springer Science and Business Media LLC - Tập 6 Số 4 - Trang 341-347 - 2002
Isabella Di Lernia, Chiara Schiraldi, M. Generoso, Mario De Rosa
Expression and functional analysis of two NhaD type antiporters from the halotolerant and alkaliphilic Halomonas sp. Y2
Springer Science and Business Media LLC - Tập 20 - Trang 631-639 - 2016
Yanbing Cui, Bin Cheng, Yiwei Meng, Chunfang Li, Huijia Yin, Ping Xu, Chunyu Yang
Na+/H+ antiporters play important roles in ion and pH homeostasis. In this study, two NhaD homologues that effectively catalyze Na+/H+ antiporter were identified from Halomonas sp. Y2, a halotolerant and alkaliphilic strain isolated from sodium enriched black liquor. They exhibited high sequence identity of 72 % and similar binding affinities for Na+ and Li+ translocation, while having different p...... hiện toàn bộ
How hyperthermophiles adapt to change their lives: DNA exchange in extreme conditions
Springer Science and Business Media LLC - Tập 17 - Trang 545-563 - 2013
Marleen van Wolferen, Małgorzata Ajon, Arnold J. M. Driessen, Sonja-Verena Albers
Transfer of DNA has been shown to be involved in genome evolution. In particular with respect to the adaptation of bacterial species to high temperatures, DNA transfer between the domains of bacteria and archaea seems to have played a major role. In addition, DNA exchange between similar species likely plays a role in repair of DNA via homologous recombination, a process that is crucial under DNA ...... hiện toàn bộ
Bifunctional recombinant cellulase–xylanase (rBhcell-xyl) from the polyextremophilic bacterium Bacillus halodurans TSLV1 and its utility in valorization of renewable agro-residues
Springer Science and Business Media LLC - Tập 20 - Trang 831-842 - 2016
Gurdeep Rattu, Swati Joshi, T. Satyanarayana
The thermostable bifunctional CMCase and xylanase encoding gene (rBhcell-xyl) from Bacillus halodurans TSLV1 has been expressed in Escherichia coli. The recombinant E. coli produced rBhcell-xyl (CMCase 2272 and 910 U L−1 xylanase). The rBhcell-xyl is a ~62-kDa monomeric protein with temperature and pH optima of 60 °C and 6.0 with T1/2 of 7.0 and 3.5 h at 80 °C for CMCase and xylanase, respectively...... hiện toàn bộ
Amino acid contacts in proteins adapted to different temperatures: hydrophobic interactions and surface charges play a key role
Springer Science and Business Media LLC - - 2008
Gisle Sælensminde, Øyvind Halskau, Inge Jonassen
Thermophiles, mesophiles, and psychrophiles have different amino acid frequencies in their proteins, probably because of the way the species adapt to very different temperatures in their environment. In this paper, we analyse how contacts between sidechains vary between homologous proteins from species that are adapted to different temperatures, but displaying relatively high sequence similarity. ...... hiện toàn bộ
A simple procedure to determine the infectivity and host range of viruses infecting anaerobic and hyperthermophilic microorganisms
Springer Science and Business Media LLC - Tập 17 - Trang 349-355 - 2013
Aurore Gorlas, Claire Geslin
Plaque assay is the method traditionally used to isolate and purify lytic viruses, to determine the viral titer and host range. Whereas most bacterioviruses are either temperate or lytic, the majority of known archeoviruses are not lytic (i.e. they are temperate or chronic). In view of the widespread occurrence of such viruses in extreme environments, we designed an original method, called the inv...... hiện toàn bộ
Biochemical and structural studies of a l-haloacid dehalogenase from the thermophilic archaeon Sulfolobus tokodaii
Springer Science and Business Media LLC - Tập 13 - Trang 179-190 - 2008
Carrie A. Rye, Michail N. Isupov, Andrey A. Lebedev, Jennifer A. Littlechild
Haloacid dehalogenases have potential applications in the pharmaceutical and fine chemical industry as well as in the remediation of contaminated land. The l-2-haloacid dehalogenase from the thermophilic archaeon Sulfolobus tokodaii has been cloned and over-expressed in Escherichia coli and successfully purified to homogeneity. Here we report the structure of the recombinant dehalogenase solved by...... hiện toàn bộ
Functional and structural characterization of the minimal Sec translocase of the hyperthermophile Thermotoga maritima
Springer Science and Business Media LLC - Tập 9 - Trang 307-316 - 2005
Monika G. Pretz, Hervé Remigy, Jelto Swaving, Sonja V. Albers, Victoria G. Garrido, Mohamed Chami, Andreas Engel, Arnold J. M. Driessen
The genome of the hyperthermophilic bacterium Thermotoga maritima contains the genes that encode core subunits of the protein translocase, a complex consisting of the molecular motor SecA and the protein conducting pore SecYE. In addition, we identified an erroneous sequence in the genome encoding for a putative secG gene. The genes of the T. maritima translocase subunits were overexpressed in Esc...... hiện toàn bộ
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