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A simple procedure to determine the infectivity and host range of viruses infecting anaerobic and hyperthermophilic microorganisms
Springer Science and Business Media LLC - Tập 17 - Trang 349-355 - 2013
Plaque assay is the method traditionally used to isolate and purify lytic viruses, to determine the viral titer and host range. Whereas most bacterioviruses are either temperate or lytic, the majority of known archeoviruses are not lytic (i.e. they are temperate or chronic). In view of the widespread occurrence of such viruses in extreme environments, we designed an original method, called the inv......
Functional and structural characterization of the minimal Sec translocase of the hyperthermophile Thermotoga maritima
Springer Science and Business Media LLC - Tập 9 - Trang 307-316 - 2005
The genome of the hyperthermophilic bacterium Thermotoga maritima contains the genes that encode core subunits of the protein translocase, a complex consisting of the molecular motor SecA and the protein conducting pore SecYE. In addition, we identified an erroneous sequence in the genome encoding for a putative secG gene. The genes of the T. maritima translocase subunits were overexpressed in Esc......
Characterization of cofactor-dependent and cofactor-independent phosphoglycerate mutases from Archaea
Springer Science and Business Media LLC - Tập 11 - Trang 647-657 - 2007
Phosphoglycerate mutases (PGM) catalyze the reversible conversion of 3-phosphoglycerate and 2-phosphoglycerate as part of glycolysis and gluconeogenesis. Two structural and mechanistically unrelated types of PGMs are known, a cofactor (2,3-bisphosphoglycerate)-dependent (dPGM) and a cofactor-independent enzyme (iPGM). Here, we report the characterization of the first archaeal cofactor-dependent PG......
Thermoactive extracellular proteases of Geobacillus caldoproteolyticus, sp. nov., from sewage sludge
Springer Science and Business Media LLC - Tập 8 - Trang 489-498 - 2004
A proteolytic thermophilic bacterial strain, designated as strain SF03, was isolated from sewage sludge in Singapore. Strain SF03 is a strictly aerobic, Gram stain-positive, catalase-positive, oxidase-positive, and endospore-forming rod. It grows at temperatures ranging from 35 to 65°C, pH ranging from 6.0 to 9.0, and salinities ranging from 0 to 2.5%. Phylogenetic analyses revealed that strain SF......
Solid-state fermentation as a potential technique for esterase/lipase production by halophilic archaea
Springer Science and Business Media LLC - Tập 19 - Trang 1121-1132 - 2015
Halophilic archaea are extremophiles, adapted to high-salt environments, showing a big biotechnological potential as enzyme, lipids and pigments producers. Four inert supports (perlite, vermiculite, polyurethane foam and glass fiber) were employed for solid-state fermentation (SSF) of the halophilic archaeon Natronococcus sp. TC6 to investigate biomass and esterase production. A very low esterase ......
Gluconeotrehalose is the principal organic solute in the psychrotolerant bacterium Carnobacterium strain 17-4
Springer Science and Business Media LLC - Tập 15 - Trang 463-472 - 2011
A high proportion of microorganisms that colonise cold environments originate from marine sites; hence, they must combine adaptation to low temperature with osmoregulation. However, little or nothing is known about the nature of compatible solutes used by cold-adapted organisms to balance the osmotic pressure of the external medium. We studied the intracellular accumulation of small organic solute......
Identification of thermoacidophilic bacteria and a new Alicyclobacillus genomic species isolated from acidic environments in Japan
Springer Science and Business Media LLC - Tập 6 - Trang 333-340 - 2002
Sixty strains of thermoacidophilic bacteria have been isolated from soil and water samples obtained from various acidic environments in Japan. An initial comparative sequence analysis of the hypervariable regions of the 16S rDNA revealed that all strains could be assigned to the Alicyclobacillus acidocaldarius–Alicyclobacillus genomic species 1 group, which could be further subdivided into three c......
Cold-active serine alkaline protease from the psychrophilic bacterium Pseudomonas strain DY-A: enzyme purification and characterization
Springer Science and Business Media LLC - Tập 7 - Trang 335-337 - 2003
An extracellular protease was purified from a deep-sea psychrophilic bacterium strain DY-A which was identified as a Pseudomonas species. The optimal growth and protease-producing temperatures of the strain were all 10°C, and the protease was secreted only at temperatures under 20°C. The enzyme was most active at 40°C and at pH 10.0. It was inhibited by phenylmethyl sulfonylfluoride and diisopropy......
Transcriptional response to copper excess and identification of genes involved in heavy metal tolerance in the extremophilic microalga Chlamydomonas acidophila
Springer Science and Business Media LLC - Tập 19 - Trang 657-672 - 2015
High concentrations of heavy metals are typical of acidic environments. Therefore, studies on acidophilic organisms in their natural environments improve our understanding on the evolution of heavy metal tolerance and detoxification in plants. Here we sequenced the transcriptome of the extremophilic microalga Chlamydomonas acidophila cultivated in control conditions and with 500 μM of copper for 2......
Cloning and characterization of a cold-active xylanase enzyme from an environmental DNA library
Springer Science and Business Media LLC - Tập 10 - Trang 295-300 - 2006
There is a great interest in xylanases due to the wide variety of industrial applications for these enzymes. We cloned a xylanase gene (xyn8) from an environmental genomic DNA library. The encoded enzyme was predicted to be 399 amino acids with a molecular weight of 45.9 kD. The enzyme was categorized as a glycosyl hydrolase family 8 member based on sequence analysis of the putative catalytic doma......
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