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Intragenomic diversity of the V1 regions of 16S rRNA genes in high-alkaline protease-producing Bacillus clausii spp
Springer Science and Business Media LLC - Tập 11 - Trang 597-603 - 2007
Alkaliphilic Bacillus sp. strain KSM-K16, which produces high-alkaline
M-protease, was characterized phenotypically, biochemically and genetically.
This strain was identified as Bacillus clausii based on the results of taxonomic
studies, including sequencing of the 16S rRNA gene and DNA-DNA hybridization.
Seven rRNA operons in the genome were identified by pulsed-field gel
electrophoresis. Sequenc...
A denitrifying bacterium from the deep sea at 11 000-m depth
Springer Science and Business Media LLC - Tập 1 - Trang 207-211 - 1997
The denitrifying bacterium strain MT-1 was isolated from the mud of the Mariana
Trench. The optimal temperature and pressure for growth of this bacterium were
found to be 30°C and 0.1 MPa, respectively. However, it showed greater tolerance
to low temperature (4°C) and high hydrostatic pressure (50 MPa) as compared with
denitrifiers obtained from land. From the results, it can be said that this
org...
Trehalose production at high temperature exploiting an immobilized cell bioreactor
Springer Science and Business Media LLC - Tập 6 Số 4 - Trang 341-347 - 2002
Expression and functional analysis of two NhaD type antiporters from the halotolerant and alkaliphilic Halomonas sp. Y2
Springer Science and Business Media LLC - Tập 20 - Trang 631-639 - 2016
Na+/H+ antiporters play important roles in ion and pH homeostasis. In this
study, two NhaD homologues that effectively catalyze Na+/H+ antiporter were
identified from Halomonas sp. Y2, a halotolerant and alkaliphilic strain
isolated from sodium enriched black liquor. They exhibited high sequence
identity of 72 % and similar binding affinities for Na+ and Li+ translocation,
while having different p...
How hyperthermophiles adapt to change their lives: DNA exchange in extreme conditions
Springer Science and Business Media LLC - Tập 17 - Trang 545-563 - 2013
Transfer of DNA has been shown to be involved in genome evolution. In particular
with respect to the adaptation of bacterial species to high temperatures, DNA
transfer between the domains of bacteria and archaea seems to have played a
major role. In addition, DNA exchange between similar species likely plays a
role in repair of DNA via homologous recombination, a process that is crucial
under DNA ...
Bifunctional recombinant cellulase–xylanase (rBhcell-xyl) from the polyextremophilic bacterium Bacillus halodurans TSLV1 and its utility in valorization of renewable agro-residues
Springer Science and Business Media LLC - Tập 20 - Trang 831-842 - 2016
The thermostable bifunctional CMCase and xylanase encoding gene (rBhcell-xyl)
from Bacillus halodurans TSLV1 has been expressed in Escherichia coli. The
recombinant E. coli produced rBhcell-xyl (CMCase 2272 and 910 U L−1 xylanase).
The rBhcell-xyl is a ~62-kDa monomeric protein with temperature and pH optima of
60 °C and 6.0 with T1/2 of 7.0 and 3.5 h at 80 °C for CMCase and xylanase,
respectively...
Amino acid contacts in proteins adapted to different temperatures: hydrophobic interactions and surface charges play a key role
Springer Science and Business Media LLC - - 2008
Thermophiles, mesophiles, and psychrophiles have different amino acid
frequencies in their proteins, probably because of the way the species adapt to
very different temperatures in their environment. In this paper, we analyse how
contacts between sidechains vary between homologous proteins from species that
are adapted to different temperatures, but displaying relatively high sequence
similarity. ...
A simple procedure to determine the infectivity and host range of viruses infecting anaerobic and hyperthermophilic microorganisms
Springer Science and Business Media LLC - Tập 17 - Trang 349-355 - 2013
Plaque assay is the method traditionally used to isolate and purify lytic
viruses, to determine the viral titer and host range. Whereas most
bacterioviruses are either temperate or lytic, the majority of known
archeoviruses are not lytic (i.e. they are temperate or chronic). In view of the
widespread occurrence of such viruses in extreme environments, we designed an
original method, called the inv...
Biochemical and structural studies of a l-haloacid dehalogenase from the thermophilic archaeon Sulfolobus tokodaii
Springer Science and Business Media LLC - Tập 13 - Trang 179-190 - 2008
Haloacid dehalogenases have potential applications in the pharmaceutical and
fine chemical industry as well as in the remediation of contaminated land. The
l-2-haloacid dehalogenase from the thermophilic archaeon Sulfolobus tokodaii has
been cloned and over-expressed in Escherichia coli and successfully purified to
homogeneity. Here we report the structure of the recombinant dehalogenase solved
by...
Functional and structural characterization of the minimal Sec translocase of the hyperthermophile Thermotoga maritima
Springer Science and Business Media LLC - Tập 9 - Trang 307-316 - 2005
The genome of the hyperthermophilic bacterium Thermotoga maritima contains the
genes that encode core subunits of the protein translocase, a complex consisting
of the molecular motor SecA and the protein conducting pore SecYE. In addition,
we identified an erroneous sequence in the genome encoding for a putative secG
gene. The genes of the T. maritima translocase subunits were overexpressed in
Esc...
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