Science in China Series C: Life Sciences

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Transfer of small chromosome fragments ofAgropyron elongatum to wheat chromosome via asymmetric somatic hybridization
Science in China Series C: Life Sciences - Tập 47 - Trang 434-441 - 2004
Jing Wang, Fengning Xiang, Guangmin Xia, Huimin Chen
The chromosome constitution of hybrids and chromatin patterns of Agropyron elongatum (Host)Neviski in F5 somatic hybrid lines -1–3 and I-1-9 between Triticum aestivum L. and A. elongatum were analyzed. Based on the statistic data of pollen mother cells, F5 I-1-9 and-1-3 had 20–21 bivalents with a frequency of 84.66% and 85.28%, of which, 89.83% and 89.57% were ring bivalents. The result indicated ...... hiện toàn bộ
Effect of lysophosphatidylcholine on behavior and structure of phosphatidylcholine liposomes
Science in China Series C: Life Sciences - Tập 40 - Trang 622-629 - 1997
Jingze Lu, Yumin Xu, Jianwen Chen, Fen Huang
Differential scanning calorimetry (DSC), fluorescence polarization and X-ray diffraction were performed to investigate the kinetics of the micellar to the lamellar phase transition of dipalmitoylphosphatidylcholine/1-palmitoylphosphatidylcholine (16:0 LPC/DPPC) liposomes at gel phase. With a 16:0 LPC concentration up to 27 mol% only the sharp main transition with relatively high enthalpy (ΔH) valu...... hiện toàn bộ
Multi-isomorphous replacement phasing of the earthworm fibrinolytic enzyme component A from Eisenia fetida
Science in China Series C: Life Sciences - Tập 46 - Trang 263-272 - 2003
Yong Tang, Tao Jiang, Jiping Zhang, Rong Fan, Cheng Wu, Dongcai Liang, Wenrui Chang
Earthworm fibrinolytic enzyme component A (EFEa) from Eisenia fetida, a protein functioning not only as a direct fibrinolytic enzyme, but also as a plasminogen activator, has been crystallized in P212121 space group with 3 protein molecules per asymmetric unit. Four heavy atom derivatives were prepared using a mother liquor containing 1.4 mol · L-1 Li2SO4 and 0.1 mol · L-1 MOPS buffer (pH7.2) and ...... hiện toàn bộ
Neural precursors derived from human embryonic stem cells
Science in China Series C: Life Sciences - Tập 48 - Trang 295-299 - 2005
Hongmei Peng, Gui’an Chen
Human embryonic stem (hES) cells provide a promising supply of specific cell types for transplantation therapy. We presented here the method to induce differentiation of purified neural precursors from hES cells. hES cells (Line PKU-1 and Line PKU-2) were cultured in suspension in bacteriological Petri dishes, which differentiated into cystic embryoid bodies (EBs). The EBs were then cultured in N2...... hiện toàn bộ
The microarray analysis for gene expression in haploids and diploids derived from twin-seedling rice
Science in China Series C: Life Sciences - Tập 51 - Trang 503-512 - 2008
HongYu Zhang, Hai Peng, PingChuan Li, QiMing Deng, PeiZhou Xu, Yun Li, XuDong Wang, XianJun Wu
In this study, microarray technique was employed to analyze the gene expression at the RNA level between haploids and corresponding diploids derived from a rice twin-seedling line SARII-628. Different degrees of expression variations were observed in the plant after haploidization. The main results are as follows: (1) after haploidization, the ratio of the sensitive loci was 2.47% of the total loc...... hiện toàn bộ
Study on the determinants of suckling mice neurovirulence of dengue 2 virus
Science in China Series C: Life Sciences - Tập 46 Số 1 - Trang 95-103 - 2003
Wei Zhao, Baochao Fan, Zhijun Hu, Suiping Chen, Pengcheng Wang, Xitong Yuan, Xiaoyü Li, Man Yu, E-De Qin, Pei Yang
Genome size and sequence composition of moso bamboo: A comparative study
Science in China Series C: Life Sciences - Tập 50 - Trang 700-705 - 2007
YiJie Gui, Sheng Wang, LiYan Quan, ChangPing Zhou, ShiBao Long, HuaJun Zheng, Liang Jin, XianYin Zhang, NaiXun Ma, LongJiang Fan
Moso bamboo (Phyllostachys pubescens) is one of the world’s most important bamboo species. It has the largest area of all planted bamboo—over two-thirds of the total bamboo forest area—and the highest economic value in China. Moso bamboo is a tetraploid (4x=48) and a special member of the grasses family. Although several genomes have been sequenced or are being sequenced in the grasses family, we ...... hiện toàn bộ
Conservation of ribosomal protein gene ordering in 16 complete genomes
Science in China Series C: Life Sciences - - 2000
Ning Wang, Runsheng Chen, Yongxiong Wang
The organization of ribosomal proteins in 16 prokaryotic genomes was studied as an example of comparative genome analyses of gene systems. Hypothetical ribosomal protein-containing operons were constructed. These operons also contained putative genes and other non-ribosomal genes. The correspondences among these genes across different organisms were clarified by sequence homology computations. In ...... hiện toàn bộ
Crystal structure determination of a neutral neurotoxin BmK M4 fromButhus martensii Karsch at 0.20 nm
Science in China Series C: Life Sciences - - 2000
Xiaolin He, Xinqi Liu, Z. Zeng, Hongmin Li, Miao Wang, Ying Zhang, Dacheng Wang
Cloned calves produced by nuclear transfer from cultured cumulus cells
Science in China Series C: Life Sciences - Tập 45 - Trang 201-210 - 2002
Xiaorong An, Kemian Gou, Shien Zhu, Hong Guan, Jian Hou, Aixing Lin, Shenming Zeng, Jianhui Tian, Yongfu Chen
Short-term cultured cumulus cell lines (1-5BCC) derived from 5 individual cows were used in nuclear transfer (NT) and 1188 enucleated bovine oocytes matured in vitro were used as nuclear recipients. A total of 931 (78.4%) cloned embryos were reconstructed, of which 763 (82%) cleaved, 627 (67.3%) developed to 8-cell stage, and 275 (29.5%) reached blastocyst stage. The average cell number of blastoc...... hiện toàn bộ
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