Science in China Series C: Life Sciences

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Transfer of small chromosome fragments ofAgropyron elongatum to wheat chromosome via asymmetric somatic hybridization
Science in China Series C: Life Sciences - Tập 47 - Trang 434-441 - 2004
Jing Wang, Fengning Xiang, Guangmin Xia, Huimin Chen
The chromosome constitution of hybrids and chromatin patterns of Agropyron elongatum (Host)Neviski in F5 somatic hybrid lines -1–3 and I-1-9 between Triticum aestivum L. and A. elongatum were analyzed. Based on the statistic data of pollen mother cells, F5 I-1-9 and-1-3 had 20–21 bivalents with a frequency of 84.66% and 85.28%, of which, 89.83% and 89.57% were ring bivalents. The result indicated ... hiện toàn bộ
Role of trichome ofPteris vittata L. in arsenic hyperaccumulation
Science in China Series C: Life Sciences - - 2005
Wenxue Li, Tongbin Chen, Yang Chen, Mei Lei
Raman microspectroscopic study of biomolecular structure inside living adhesive cells
Science in China Series C: Life Sciences - Tập 45 - Trang 397-405 - 2002
Guang Li, Hongying Yang, Yiming Xu, Zhiyi Zhang
Cells adhesion is very important for many physiological processes. Using advanced Raman microspectroscopic technique, we selected T Leukemia cells (Jurkat) as the materials and obtained simultaneously conformation information of various biomolecules inside the whole living cells. By comparing the Raman microspectroscopic spectra of single and adhesive cancer cells, we found for the first time that... hiện toàn bộ
In vitro flowering ofDendrobium candidum
Science in China Series C: Life Sciences - Tập 40 - Trang 35-42 - 1997
Guangyuan Wang, Zhihong Xu, Tet-Fatt Chia, Nam-Hai Chua
Dendrobium candidum, a wild orchid species from China, normally requires three to four years of cultivation before it can produce flowers. The effects of plant hormones and polyamines on flower initiation of this species in tissue culture were investigated. The addition of spermidine, or BA, or the combination of NAA and BA to the culture medium can induce protocorms or shoots to flower within thr... hiện toàn bộ
Effect of lysophosphatidylcholine on behavior and structure of phosphatidylcholine liposomes
Science in China Series C: Life Sciences - Tập 40 - Trang 622-629 - 1997
Jingze Lu, Yumin Xu, Jianwen Chen, Fen Huang
Differential scanning calorimetry (DSC), fluorescence polarization and X-ray diffraction were performed to investigate the kinetics of the micellar to the lamellar phase transition of dipalmitoylphosphatidylcholine/1-palmitoylphosphatidylcholine (16:0 LPC/DPPC) liposomes at gel phase. With a 16:0 LPC concentration up to 27 mol% only the sharp main transition with relatively high enthalpy (ΔH) valu... hiện toàn bộ
Multi-isomorphous replacement phasing of the earthworm fibrinolytic enzyme component A from Eisenia fetida
Science in China Series C: Life Sciences - Tập 46 - Trang 263-272 - 2003
Yong Tang, Tao Jiang, Jiping Zhang, Rong Fan, Cheng Wu, Dongcai Liang, Wenrui Chang
Earthworm fibrinolytic enzyme component A (EFEa) from Eisenia fetida, a protein functioning not only as a direct fibrinolytic enzyme, but also as a plasminogen activator, has been crystallized in P212121 space group with 3 protein molecules per asymmetric unit. Four heavy atom derivatives were prepared using a mother liquor containing 1.4 mol · L-1 Li2SO4 and 0.1 mol · L-1 MOPS buffer (pH7.2) and ... hiện toàn bộ
The influence of Annexin32, a new Ca2+-dependent phospholipid-binding protein, on coagulation time and thrombosis
Science in China Series C: Life Sciences - Tập 45 - Trang 186-190 - 2002
Yi Zhang, Ruiwen Chen, Shuhan Sun
The pharmacodynamics of Annexin32, a new Ca2+-dependent phospholipid-binding protein, was studied by measuring coagulation time in rabbits and venous thrombosis in rabbits and rats. Rabbits and rats were given Annexin32 by intravenous administration. Then Kaolin partial thromboplastin time (KPTT), thrombosis in vitro and in vivo were assayed. The results showed that KPTT of rabbits was prolonged (... hiện toàn bộ
Neural precursors derived from human embryonic stem cells
Science in China Series C: Life Sciences - Tập 48 - Trang 295-299 - 2005
Hongmei Peng, Gui’an Chen
Human embryonic stem (hES) cells provide a promising supply of specific cell types for transplantation therapy. We presented here the method to induce differentiation of purified neural precursors from hES cells. hES cells (Line PKU-1 and Line PKU-2) were cultured in suspension in bacteriological Petri dishes, which differentiated into cystic embryoid bodies (EBs). The EBs were then cultured in N2... hiện toàn bộ
The microarray analysis for gene expression in haploids and diploids derived from twin-seedling rice
Science in China Series C: Life Sciences - Tập 51 - Trang 503-512 - 2008
HongYu Zhang, Hai Peng, PingChuan Li, QiMing Deng, PeiZhou Xu, Yun Li, XuDong Wang, XianJun Wu
In this study, microarray technique was employed to analyze the gene expression at the RNA level between haploids and corresponding diploids derived from a rice twin-seedling line SARII-628. Different degrees of expression variations were observed in the plant after haploidization. The main results are as follows: (1) after haploidization, the ratio of the sensitive loci was 2.47% of the total loc... hiện toàn bộ
Nitrogen deposition and reduction of terrestrial biodiversity: Evidence from temperate grasslands
Science in China Series C: Life Sciences - Tập 48 - Trang 720-728 - 2005
Nancy B. Dise, J. Stevens
Biodiversity is thought to be essential for ecosystem stability, function and long-term sustainability. Since nitrogen is the limiting nutrient for plant growth in many terrestrial ecosystems, reactive nitrogen has the potential to reduce the diversity of terrestrial vegetation and associated biota through favouring species adapted to quickly exploiting available nutrients. Although the potential ... hiện toàn bộ
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