Science in China Series C: Life Sciences
Công bố khoa học tiêu biểu
* Dữ liệu chỉ mang tính chất tham khảo
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Raman spectroscopic study of space structure of membrane proteins and membrane lipids in photodamaged human erythrocyte sensitized by hypocrellin B
Science in China Series C: Life Sciences - - 1998
Chromate and selenate hydrocalumite solid solutions and their applications in waste treatment
Science in China Series C: Life Sciences - Tập 48 - Trang 165-173 - 2005
Hydrocalumite, a calcium aluminate hydrate phase, consists of positively-charged structure units, and is therefore an ideal candidate for accommodating anionic contaminants. In this study, a series of batch experiments was carried out to examine the uptake of chromate and selenate by hydrocalumite. To determine the uptake capacity and long-term stability, hydrocalumite solid solutions between chro...... hiện toàn bộ
Construction of antisense RNA expression vectors and correction of splicing defect in human β-globin gene (IVS-2-654 C→T mutant) in HeLa cells
Science in China Series C: Life Sciences - Tập 41 - Trang 99-106 - 1998
The antisense fragments, which were available inin vitro system, were cloned into the mammalian expression vector pcDNA3, and were transfected into H654 cells, a mammalian cell line stably expressing the thalassaemic (IVS-2-654 C→T) human β-globin gene. In these transfected cells, the level of correctly spliced β-globin mRNA in total β-globin mRNA (β/(β + β*)) was improved from 0.07 (0 d) to 0.22 ...... hiện toàn bộ
Expression of betaine aldehyde dehydrogenase gene and salinity tolerance in rice transgenic plants
Science in China Series C: Life Sciences - Tập 40 - Trang 496-501 - 1997
Betaine as one of osmolytes plays an important role in osmoregulation of most high plants. Betaine aldehyde dehydrogenase (BADH) is the second enzyme involved in betaine biosynthesis. The BADH gene from a halophite,Atriplex hortensis, was transformed into rice cultivars by bombarment method. Totally 192 transgenic rice plants were obtained and most of them had higher salt tolerance than controls. ...... hiện toàn bộ
The transfer RNA genes in Oryza sativa L. ssp. indica
Science in China Series C: Life Sciences - Tập 45 - Trang 504-511 - 2002
The availability of the draft genome sequence of Oryza sativa L. ssp. indica has made it possible to study the rice tRNA genes. A total of 596 tRNA genes, including 3 selenocysteine tRNA genes and one suppressor tRNA gene are identified in 127551 rice contigs. There are 45 species of tRNA genes and the revised wobble hypothesis proposed by Guthrie and Abelson is perfectly obeyed. The relationship ...... hiện toàn bộ
The influence of Annexin32, a new Ca2+-dependent phospholipid-binding protein, on coagulation time and thrombosis
Science in China Series C: Life Sciences - Tập 45 - Trang 186-190 - 2002
The pharmacodynamics of Annexin32, a new Ca2+-dependent phospholipid-binding protein, was studied by measuring coagulation time in rabbits and venous thrombosis in rabbits and rats. Rabbits and rats were given Annexin32 by intravenous administration. Then Kaolin partial thromboplastin time (KPTT), thrombosis in vitro and in vivo were assayed. The results showed that KPTT of rabbits was prolonged (...... hiện toàn bộ
Effects of TFAR19 gene on the growth and biorheological properties of mouse erythroleukemia cell line MEL
Science in China Series C: Life Sciences - Tập 46 - Trang 293-301 - 2003
Using the method of gene transfection with liposome, we obtained the mouse erythroleukemia cell line MEL-TF19, which stably carries TFAR19, a novel apoptosis-related gene. The expression of TFAR19 was detected by Western blot. Growth curve and flow cytometry analysis showed that after being transfected with TFAR19 gene, the growth of MEL-TF19 is suppressed and its apoptosis is accelerated because ...... hiện toàn bộ
Cloning and biological comparison of Restin, novel member of Mage superfamily
Science in China Series C: Life Sciences - Tập 45 - Trang 412-420 - 2002
In the present study, a new member of melanoma associated antigens (Mage), named Restin (219 amino acids), was identified from HL-60 cell induced by all-trans-retinoic acid (ATRA) by PCR-based subtractive hybridization. Bioinformatics analysis found this novel gene shares high homolog with Necdin (a neuronal growth suppressor, 49%). Both of them are basic proteins. Moreover, the Restin, Necdin and...... hiện toàn bộ
Increasing drug resistance in human lung cancer cells by mutant-type p53 gene mediated by retrovirus
Science in China Series C: Life Sciences - Tập 40 - Trang 101-106 - 1997
Human mutant-type (mt) p53 cDNA was synthesized and cloned from human lung cancer cell line GL containing mt-p53 gene by using polymerase chain reaction (PCR). It was confirmed that the mt-p53 cDNA contained the complete coding sequence of p53 gene but mutated at codon 245 (G→T) and resulted in glycine to cysteine by sequencing analysis. The retroviral vector pD53M of the mt-p53 was constructed an...... hiện toàn bộ
Sequence analysis of leader and trailer regions of rice yellow stunt rhabdovirus and characterization of theirin vivo transcripts
Science in China Series C: Life Sciences - Tập 42 - Trang 50-56 - 1999
The 3′ leader and the 5′ trailer of the rice yellow stunt rhabdovirus (RYSV) genomic RNA have been cloned and sequenced. Sequence data indicate that the RYSV leader region is composed of 203 nucleotides (nt) and the trailer region 191 nt. The terminal 9 nt of the two regions are complementary and capable of forming a putative panhandle structure common to rhabdovirus genomes. In comparison with th...... hiện toàn bộ
Tổng số: 857
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