Developmental and regional variations in ribonucleic acid synthesis on cerebral chromatinPortland Press Ltd. - Tập 115 Số 2 - Trang 341-349 - 1969
Stephen C. Bondy, Sidney Roberts
1. Chromatin was prepared from purified nuclei isolated from liver and cerebral regions of the rat. 2. The capacity of these preparations to promote RNA synthesis in the presence of bacterial RNA polymerase was determined. 3. The rate of RNA synthesis on chromatin was normally 12–21% of the rate observed with native DNA, but was markedly stimulated on addition of 200mm-ammonium sulphate. 4...... hiện toàn bộ
Isomers of glucosaminylphosphatidylglycerol in Bacillus megateriumPortland Press Ltd. - Tập 114 Số 2 - Trang 361-367 - 1969
Jamie MacDougall, P. J. R. Phizackerley
1. The lipids of Bacillus megaterium were extracted and three lipids containing glucosamine were identified. One of these is not a phospholipid, but the other two, which differ in their chromatographic behaviour, contain phosphorus, glycerol, fatty acid and d-glucosamine in the molar proportions 1:2:2:1. 2. In both phosphoglycolipids, the fatty acids are bound in ester linkage, and both yi...... hiện toàn bộ
The activity of glycogen synthase phosphatase limits hepatic glycogen deposition in the adrenalectomized starved ratPortland Press Ltd. - Tập 214 Số 2 - Trang 539-545 - 1983
Mathieu Bollen, G Gevers, Willy Stalmans
Hepatocytes from adrenalectomized 48 h-starved rats responded to increasing glucose concentrations with a progressively more complete inactivation of phosphorylase. Yet no activation of glycogen synthase occurred, even in a K+-rich medium. Protein phosphatase activities in crude liver preparations were assayed with purified substrates. Adrenalectomy plus starvation decreased synthase phosp...... hiện toàn bộ
Restricted permeability of rat liver for glutamate and succinatePortland Press Ltd. - Tập 107 Số 6 - Trang 807-815 - 1968
R. Hems, Marion Stubbs, H. A. Krebs
1. When rat liver slices were incubated aerobically with [U−14C]glutamate the concentration of 14C within the slices remained lower (about 50%) than in the medium. The maximal concentration of 14C in the liver was reached within minutes. In rat kidney-cortex slices by contrast, 14C reached concentrations more than six times those of the medium. 2. In both liver and kidney 14C appeared in t...... hiện toàn bộ
Studies of the denaturation and partial renaturation of ovalbuminPortland Press Ltd. - Tập 129 Số 3 - Trang 665-676 - 1972
John Clifford Holt, J. M. Creeth
1. The denaturation of ovalbumin by the reagents sodium dodecyl sulphate and guanidinium chloride was investigated, by following the changes in sedimentation velocity, optical rotatory dispersion and viscosity as a function of denaturant concentration. 2. With sodium dodecyl sulphate both the optical-rotatory-dispersion parameters a0 and b0 become more negative, the sedimentation coefficie...... hiện toàn bộ
Respiration-driven proton translocation in rat liver mitochondriaPortland Press Ltd. - Tập 105 Số 3 - Trang 1147-1162 - 1967
Peter Mitchell, Jennifer Moyle
1. Pulses of acidity of the outer aqueous phase of rat liver mitochondrial suspensions induced by pulses of respiration are due to the translocation of H+ (or OH−) ions across the osmotic barrier (M phase) of the cristae membrane and cannot be attributed to the formation (with acid production) of a chemical intermediate that subsequently decomposes. 2. The effective quantity of protons tra...... hiện toàn bộ
Effect of growth hormone on fibrinogen synthesisPortland Press Ltd. - Tập 119 Số 2 - Trang 243-249 - 1970
Khursheed N. Jeejeebhoy, Alan Bruce-Robertson, U. Sodtke, M. Foley
A method described by McFarlane (1963a) for the measurement of the absolute rate of synthesis of liver-made plasma proteins was used to show that, within a few hours of giving bovine growth hormone to rats, fibrinogen synthesis increased significantly without a change in albumin synthesis.