Developmental and regional variations in ribonucleic acid synthesis on cerebral chromatinPortland Press Ltd. - Tập 115 Số 2 - Trang 341-349 - 1969
Stephen C. Bondy, Sidney Roberts
1. Chromatin was prepared from purified nuclei isolated from liver and cerebral regions of the rat. 2. The capacity of these preparations to promote RNA synthesis in the presence of bacterial RNA polymerase was determined. 3. The rate of RNA synthesis on chromatin was normally 12–21% of the rate observed with native DNA, but was markedly stimulated on addition of 200mm-ammonium sulphate. 4...... hiện toàn bộ
Solution structures of ferrihaem in some dipolar aprotic solvents and their binary aqueous mixturesPortland Press Ltd. - Tập 115 Số 2 - Trang 279-285 - 1969
Stanley B. Brown, I. R. Lantzke
1. Conductivity and u.v. and visible spectroscopic techniques were used to investigate the solution structure of the prosthetic group of the ferric haemoproteins (ferrihaem) in dimethyl sulphoxide, NN-dimethylacetamide, NN-dimethylformamide and sulpholane, and certain of their aqueous mixtures. 2. In neutral or acid dimethyl sulphoxide, chlorohaemin is monomeric and completely dissociated ...... hiện toàn bộ
Isomers of glucosaminylphosphatidylglycerol in Bacillus megateriumPortland Press Ltd. - Tập 114 Số 2 - Trang 361-367 - 1969
Jamie MacDougall, P. J. R. Phizackerley
1. The lipids of Bacillus megaterium were extracted and three lipids containing glucosamine were identified. One of these is not a phospholipid, but the other two, which differ in their chromatographic behaviour, contain phosphorus, glycerol, fatty acid and d-glucosamine in the molar proportions 1:2:2:1. 2. In both phosphoglycolipids, the fatty acids are bound in ester linkage, and both yi...... hiện toàn bộ
The glycerol teichoic acid from walls of Staphylococcus epidermidis I2Portland Press Ltd. - Tập 110 Số 3 - Trang 583-588 - 1968
A. R. Archibald, J. Baddiley, G. A. Shaukat
1. Walls of Staphylococcus epidermidis I2 contain 30% (w/w) of a glycerol teichoic acid containing phosphate, d-alanine and d-glucose in the molecular proportions 1:0·25:0·50. 2. The teichoic acid was isolated by extraction with trichloroacetic acid and with dilute aqueous NN-dimethylhydrazine at pH7, and was shown to be a (1→3)-linked poly(glycerol phosphate) containing β-d-glucopyranosyl...... hiện toàn bộ
The activity of glycogen synthase phosphatase limits hepatic glycogen deposition in the adrenalectomized starved ratPortland Press Ltd. - Tập 214 Số 2 - Trang 539-545 - 1983
Mathieu Bollen, G Gevers, Willy Stalmans
Hepatocytes from adrenalectomized 48 h-starved rats responded to increasing glucose concentrations with a progressively more complete inactivation of phosphorylase. Yet no activation of glycogen synthase occurred, even in a K+-rich medium. Protein phosphatase activities in crude liver preparations were assayed with purified substrates. Adrenalectomy plus starvation decreased synthase phosp...... hiện toàn bộ
Restricted permeability of rat liver for glutamate and succinatePortland Press Ltd. - Tập 107 Số 6 - Trang 807-815 - 1968
R. Hems, Marion Stubbs, H. A. Krebs
1. When rat liver slices were incubated aerobically with [U−14C]glutamate the concentration of 14C within the slices remained lower (about 50%) than in the medium. The maximal concentration of 14C in the liver was reached within minutes. In rat kidney-cortex slices by contrast, 14C reached concentrations more than six times those of the medium. 2. In both liver and kidney 14C appeared in t...... hiện toàn bộ
Studies of the denaturation and partial renaturation of ovalbuminPortland Press Ltd. - Tập 129 Số 3 - Trang 665-676 - 1972
John Clifford Holt, J. M. Creeth
1. The denaturation of ovalbumin by the reagents sodium dodecyl sulphate and guanidinium chloride was investigated, by following the changes in sedimentation velocity, optical rotatory dispersion and viscosity as a function of denaturant concentration. 2. With sodium dodecyl sulphate both the optical-rotatory-dispersion parameters a0 and b0 become more negative, the sedimentation coefficie...... hiện toàn bộ