Wheat wounding-responsive HD-Zip IV transcription factor GL7 is predominantly expressed in grain and activates genes encoding defensinsPlant Molecular Biology - Tập 101 - Trang 41-61 - 2019
Nataliya Kovalchuk, Wei Wu, Natalia Bazanova, Nicolas Reid, Rohan Singh, Neil Shirley, Omid Eini, Alexander A. T. Johnson, Peter Langridge, Maria Hrmova, Sergiy Lopato
Several classes of transcription factors are involved in the activation of defensins. A new type of the transcription factor responsible for the regulation of wheat grain specific defensins was characterised in this work. HD-Zip class IV transcription factors constitute a family of multidomain proteins. A full-length cDNA of HD-Zip IV, designated TaGL7 was isolated from the developing grain of bre...... hiện toàn bộ
Characterization and expression of Metallothionein-like genes in cottonPlant Molecular Biology - Tập 31 - Trang 701-705 - 1996
Richard L. Hudspeth, Susan L. Hobbs, David M. Anderson, Kanniah Rajasekaran, John W. Grula
We have characterized cotton (Gossypium hirsutum L.) genes encoding type 1 metallothionein-like proteins that are highly expressed in roots. Little or no expression of these genes was detected in other organs and tissues. The deduced amino acid sequences have a high degree of similarity with type 1 metallothionein-like proteins from other plants, including a central hydrophobic domain flanked by c...... hiện toàn bộ
KAP-2, a protein that binds to the H-box in a bean chalcone synthase promoter, is a novel plant transcription factor with sequence identity to the large subunit of human Ku autoantigenPlant Molecular Biology - Tập 49 - Trang 503-514 - 2002
William P. Lindsay, Fiona M. McAlister, Qun Zhu, Xian-Zhi He, Wolfgang Dröge-Laser, Susie Hedrick, Peter Doerner, Chris Lamb, Richard A. Dixon
The KAP-2 protein that binds to the H-box (CCTACC) element in the bean CHS15 chalcone synthase promoter was purified, and internal peptide sequence used to design primers leading to the cloning of KAP-2 from bean (Phaseolus vulgaris) and barrel medic (Medicago truncatula). KAP-2 shares sequence similarity to the large subunit of mammalian Ku autoantigen, a protein proposed to be involved in contro...... hiện toàn bộ
The PSI family of nuclear proteins is required for growth in arabidopsisPlant Molecular Biology - Tập 86 - Trang 289-302 - 2014
Nils Stührwohldt, Jens Hartmann, Renate I. Dahlke, Claudia Oecking, Margret Sauter
PSI1 was identified as a gene that is co-expressed with the phytosulfokine (PSK) receptor genes PSKR1 and PSKR2 in Arabidopsis thaliana. It represents a plant-specific protein family of unknown function with six members in two clades. Clade 1 members PSI1, PSI2 and PSI3 were characterized in this study. All three are nuclear localized. A predicted N-terminal myristoylation site was functionally an...... hiện toàn bộ
Characterization of mango (Mangifera indica L.) transcriptome and chloroplast genomePlant Molecular Biology - - 2014
M. Kamran Azim, Ishtaiq A. Khan, Yong Zhang
We characterized mango leaf transcriptome and chloroplast genome using next generation DNA sequencing. The RNA-seq output of mango transcriptome generated >12 million reads (total nucleotides sequenced >1 Gb). De novo transcriptome assembly generated 30,509 unigenes with lengths in the range of 300 to ≥3,000 nt and 67× depth of coverage. Blast searching against nonredundant nucleotide databases an...... hiện toàn bộ
The chloroplast DNAs of flax genotrophsPlant Molecular Biology - Tập 1 - Trang 183-189 - 1982
David Coates, Christopher A. Cullis
The chloroplast DNA of L. usitatissimum var. “Stormont Cirrus” has been mapped with respect to the recognition sites for the enzymes SalP1, Sst1 and SalG1. It is a circular molecule of about 160 kilobasepairs, with an inverted repeat containing the rDNA. Comparisons between chloroplast DNA of uninduced and induced flax genotrophs show there to be no major structural differences.
An extracellular insoluble inhibitor of cysteine proteinases in cell cultures and seeds of carrotPlant Molecular Biology - Tập 34 - Trang 99-109 - 1997
Akiko Ojima, Hajime Shiota, Katsumi Higashi, Hiroshi Kamada, Yoh-ichi Shimma, Masato Wada, Shinobu Satoh
An 18 kDa extracellular insoluble protein (EIP18) was found previously in amorphous particles suspended in the culture medium and in the interspaces of cell clusters of carrot (Daucus carota L.) callus, as well as in the extracellular spaces of carrot seeds, being located both in the embryo and at the inner edge of the endosperm. We purified EIP18 by washing the amorphous particles with the mixtur...... hiện toàn bộ
The structure and organization of the luciferase gene in the photosynthetic dinoflagellate Gonyaulax polyedraPlant Molecular Biology - Tập 36 - Trang 275-284 - 1998
Liming Li, J. Woodland Hastings
The structural features of dinoflagellate nuclei are distinct from those of other eukaryotes in several respects, and the mechanisms of DNA replication and transcription are almost completely unknown. In this study we investigated the structure and organization of the gene coding for luciferase (LCF), the enzyme catalyzing the bioluminescent reaction in the dinoflagellate Gonyaulax polyedra. The g...... hiện toàn bộ