Plant Molecular Biology Reporter
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Identifi cation of antibiotics that are effective 2 in eliminatingAgrobacterium tumefaciens
Plant Molecular Biology Reporter - Tập 14 - Trang 50-57 - 1996
An assay was performed to identify the antibiotics that are most effective againstAgrobacterium tumefaciens strains EHA101 and LBA4404, and to determine if these antibiotics inhibited tobacco callus and shoot formation. We tested ten antibiotics: cefotaxime, carbenecillin, erythromycin, spectinomycin, polymixin B, chloramphenicol, methicillin, Augmentin 500, Augmentin 250, and moxalactam. The effe...... hiện toàn bộ
Giảm thiểu ô nhiễm DNA nhân trong phân tích DNA lạp thể bằng các enzym restriction endonuclease Dịch bởi AI
Plant Molecular Biology Reporter - Tập 11 - Trang 48-53 - 1993
Nếu các lạp thể được tinh chế trên các gradient sucrose được xử lý trong 10 phút ở 4°C với 2 M NaCl, sau đó được ly tâm với lực 1000-g, ô nhiễm DNA từ nhân tế bào giảm từ 1.5 đến 3 lần như được ước tính bằng phương pháp đo mật độ quang.
#lạp thể #DNA nhân #enzyme restriction endonuclease #ô nhiễm DNA #gradient sucrose
New database for assessment of GMO food allergens
Plant Molecular Biology Reporter - Tập 18 - Trang 306-307 - 2012
Analysis of aMusa spp. somaclonal variant resistant to yellow Sigatoka
Plant Molecular Biology Reporter - Tập 18 - Trang 23-31 - 2012
Somaclonal variant CIEN BTA-03 resistant to yellow Sigatoka was obtained from a susceptible banana clone (Williams clone), by increasing the production of adventitious buds using 6-Belcilaminopurine at high concentrations. This somaclone has exhibited yellow Sigatoka resistance in the field for five consecutive years of asexual reproduction. We used RAPD markers to generate characteristic “fingerp...... hiện toàn bộ
Production of herbicide-resistant transgenic maize plants using electroporation of seed-derived embryos
Plant Molecular Biology Reporter - Tập 20 - Trang 303-304 - 2012
The improvement of commercial maize lines via biotechnological approaches is limited by the lack of a transformation system that is tissue culture free. In this paper, the development of a genetic transformation system is presented using electroporation for gene delivery and seed-derived embryo as the gene target. Plasmid DNA (pBARGUS), which contained the selectablebar gene for resistance to the ...... hiện toàn bộ
Molecular Characterization and Comparative Analysis of Six Durum Wheat Accessions Including Graziella Ra
Plant Molecular Biology Reporter - - 2012
Separation of Different Conformations of Plant Mitochondrial DNA Molecules by Field Inversion Gel Electrophoresis
Plant Molecular Biology Reporter - Tập 16 - Trang 219-229 - 1998
Mitochondrial (mt) DNA structure in higher plants is still unclear as to the circularity or linearity of the genome. We have developed a system to electrophoretically separate distinct populations of mtDNA, with some populations enriched for networked linear and circular DNA molecules. Using field inversion gel electrophoresis (FIGE) and electron microscopy (EM), we have identified four distinct p...... hiện toàn bộ
Development of TBSPG Pipelines for Refining Unique Mapping and Repetitive Sequence Detection Using the Two Halves of Each Illumina Sequence Read
Plant Molecular Biology Reporter - - 2015
We developed six pipelines (TBSPG) for mapping Illumina sequence reads to reference genomes, refining unique mapping, and computing the mapped read number and coverage. These pipelines provide the options of conducting multi-mapping or unique mapping, inputting with paired-end read files or a single-end read file, removing or not removing nucleus-organelle shared sequences, and mapping with the fu...... hiện toàn bộ
First Records on Genetic Diversity and Population Structure of Algerian Peanut (Arachis hypogaea) Using Microsatellite Markers
Plant Molecular Biology Reporter - Tập 40 - Trang 136-147 - 2021
Peanut (Arachis hypogaea L) is one of the wide cultivated plants with a narrow genetic base, hence the interest in prospecting, rescuing, and characterizing germplasm of this species is continuously carried out. In this work, eleven microsatellite markers were used to assess the genetic diversity and population structure of 68 Algerian peanut accessions originated from four geographic regions in t...... hiện toàn bộ
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