Pharmaceutical Chemistry Journal

Biochemical features of wild common cocklebur (Xanthium strumarium L.) were studied in order to assess its possible cultivation. Analyses showed high contents of fatty oil in seeds (up to 40%) and fruit (up to 12%) that consisted of unsaturated (palmitic, stearic) and more valuable polyunsaturated (linoleic, linolenic) acids. Oils extracted from seeds and whole fruit had practically identical chemical compositions. The results indicated that free iodide ion was absent in the plant and that the contents of organically bound iodine in oils from the fruit (385 mg/L) and fruit pulp after pressing (215 mg/kg) were elevated.

8-Bromo-1-iso-butyl-3-methyl-7-(thietanyl-3)xanthine (II) was synthesized by alkylation of 8-bromo-3-methyl-7-(thietanyl-3)xanthine (I) with isobutyl bromide. Nucleophilic substitution of II with thioglycolic acid gave 2{[1-iso-butyl-3-methyl-7-(thietanyl-3)xanthin-8-yl]thio}acetic acid (III). A series of water-soluble salts of 2{[1-iso-butyl-3-methyl-7-(thietanyl-3)xanthin-8-yl]thio}acetic acid (IVa-h) were synthesized by reaction of III with organic and inorganic bases. Compounds possessing antiaggregant activity on the level of acetylsalicylic acid were found among salts IV. All obtained compounds except for IVa and IVc suppressed lipid peroxidation less than ascorbic acid. However, all synthesized compounds suppressed generation of reactive oxygen species by phagocytes, in contrast to ascorbic acid.

A highly sensitive and reproducible method for assay of fluvoxamine in biological samples using HPLC combined with various detection methods (spectrophotometry and tandem mass spectrometry) was developed. These methods were completely interchangeable and correlation analysis demonstrated high consistency in the results obtained by different detection methods: the Pearson correlation coefficient was 0.982 (p < 0.0001) and the slope of the regression line was close to unity. The methods were suitable for routine therapeutic monitoring of fluvoxamine in patients’ serum.