Molecular Breeding
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Diversity of Puroindoline genes and their association with kernel hardness in Chinese wheat cultivars and landraces
Molecular Breeding - Tập 39 - Trang 1-13 - 2019
Kernel hardness is a key trait that greatly affects the milling and baking qualities of common wheat. Kernel hardness is largely controlled by Pina and Pinb genes. Here, we investigate the allelic diversity of Pina and Pinb genes in a collection of 107 Chinese wheat cultivars and landraces. The kernel hardness values of these accessions were determined in two field seasons (2016/2017 and 2017/2018......
Molecular detection of a gene effective against powdery mildew in the wheat cultivar Liangxing 66
Molecular Breeding - Tập 30 - Trang 1737-1745 - 2012
Since it was commercialized in 2008, Liangxing 66 is one of the most widely grown cultivars of wheat (Triticum aestivum L.) in winter and facultative wheat-producing regions in northern China. This cultivar displays broad-spectrum resistance to isolates of powdery mildew. To identify the powdery mildew resistance gene in Liangxing 66, genetic analysis and molecular mapping were conducted using the......
Morphological and gene expression characterization of maf-1, a floral chili pepper mutant caused by a nonsense mutation in CaLFY
Molecular Breeding - Tập 42 - Trang 1-14 - 2022
Chili peppers are important as vegetables and ornamental crops, because of the variety of fruit shapes and colors. Understanding of flower and fruit development in Capsicum is limited compared with closely related Solanaceae crops such as tomato. This study reports a novel malformed fruit mutant named malformed fruit-1 (maf-1), which was isolated from an ethyl methanesulfonate–induced mutant popul......
Inheritance and gene mapping of spotted to non-spotted trait gene CmSp-1 in melon (Cucumis melo L. var. chinensis Pangalo)
Molecular Breeding - Tập 38 - Trang 1-9 - 2018
Melon (Cucumis melo L.) is one of the most popular and highly nutritious vegetable species within Cucurbitaceae. Because appearance is used as an important indicator of quality, the spotted to non-spotted trait associated with this product somewhat influences the buying habits of consumers. We tested a six-generation family to determine the inheritance and genetic basis of this trait. Genetic grou......
Commercial production of β-glucuronidase (GUS): a model system for the production of proteins in plants
Molecular Breeding - Tập 4 - Trang 301-312 - 1998
We have generated transgenic maize seed containing β-glucuronidase(GUS) for commercial production. While many other investigators have demonstrated the expression of GUS as a scoreable marker, this is one of the first cases where a detailed characterization of the transgenic plants and the protein were performed which are necessary to use this as a commercial source of GUS. The recombinant β-glucu......
Construction of a SNP and SSR linkage map in autotetraploid blueberry using genotyping by sequencing
Molecular Breeding - Tập 36 - Trang 1-24 - 2016
The construction of the first genetic map in autotetraploid blueberry has been made possible by the development of new SNP markers developed using genotyping by sequencing in a mapping population created from a cross between two key highbush blueberry cultivars, Draper × Jewel (Vaccinium corymbosum). The novel SNP markers were supplemented with existing SSR markers to enable the alignment of paren......
Hybrid lethality caused by two complementary dominant genes in cabbage (Brassica oleracea L.)
Molecular Breeding - Tập 36 - Trang 1-10 - 2016
Hybrid lethality, a type of postzygotic reproductive barrier, is important for species. Discovering novel hybrid lethality cases and analyzing corresponding causal genes may provide new insights into the establishment and maintenance of reproductive isolation. In this study, we observed the hybrid lethality phenomena in a cross between two cabbage inbred lines, 09-211 and 09-222. Genetic analysis ......
Characterization of transcriptome and development of novel EST-SSR makers based on next-generation sequencing technology in Neolitsea sericea (Lauraceae) endemic to East Asian land-bridge islands
Molecular Breeding - Tập 35 - Trang 1-15 - 2015
Neolitsea sericea (Lauraceae), endemic to East Asian land-bridge islands, is an economically important tree species because of its characteristic aroma and timber uses. However, due to the lack of efficient molecular markers, the genetic diversity and historical demography of this endemic species is not clearly understood. In this study, we performed high-throughput transc......
Discovery of genetic loci and causal genes for seed germination via deep re-sequencing in soybean
Molecular Breeding - Tập 42 - Trang 1-16 - 2022
High seed germination is crucial for mechanical sowing, seedling establishment, growth potential, multiple resistances, and the formation of yield and quality. However, few genetic loci and candidate genes conferring seed germination were explored in soybean at present. In view of this, a natural population containing 199 accessions was assessed for the germination potential (GP) and germination r......
Molecular characterisation of the Wx-B1 allelic variants identified in cultivated emmer wheat and comparison with those of durum wheat
Molecular Breeding - Tập 28 - Trang 403-411 - 2010
Emmer wheat is a neglected crop that could be used in the breeding of modern durum wheat for quality, one important aspect of which is the starch composition that is related to the waxy proteins. A collection of 87 accessions of Spanish emmer wheat was analysed for waxy protein composition by SDS–PAGE. No polymorphism was found for the Wx-A1 gene. However, for the Wx-B1 gene, three alleles were de......
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