Molecular Breeding

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Improving accuracy of breeding values by incorporating genomic information in spatial-competition mixed models
Molecular Breeding - Tập 37 - Trang 1-13 - 2017
Eduardo P. Cappa, Yousry A. El-Kassaby, Facundo Muñoz, Martín N. Garcia, Pamela V. Villalba, Jaroslav Klápště, Susana N. Marcucci Poltri
Climate change and the increasing demand for sustainable energy resources require urgent strategies to increase the accuracy of selection in tree breeding (associated with higher gain). We investigated the combined pedigree and genomic-based relationship approach and its impact on the accuracy of predicted breeding values using data from 5-year-old Eucalyptus grandis progeny trial. The number of t...... hiện toàn bộ
Rapid marker-assisted development of advanced recombinant lines from barley starch mutants
Molecular Breeding - Tập 33 - Trang 243-248 - 2013
Phil Howell, Fiona Leigh, Ruth Bates, Nick Gosman, Kay Trafford, Wayne Powell, Alison M. Smith, Andy Greenland
The comparative phenotypic analysis of mutants is often hampered by their diverse and poorly characterised genetic backgrounds. To overcome this problem, a suite of recombinant spring barley lines was developed for four starch biosynthesis genes in a common elite background. Rapid breeding progress was made by combining foreground and background selection with the screening of bulked families. A t...... hiện toàn bộ
QTL-seq and marker development for resistance to Fusarium oxysporum f. sp. niveum race 1 in cultivated watermelon
Molecular Breeding - Tập 38 - Trang 1-9 - 2018
Sandra E. Branham, W. Patrick Wechter, Shaunese Lambel, Laura Massey, Michelle Ma, Julie Fauve, Mark W. Farnham, Amnon Levi
Fusarium wilt, caused by the fungus Fusarium oxysporum f. sp. niveum (Fon), is one of the predominant diseases of watermelon. Resistance to Fon race 1 is conferred by a single major quantitative trait locus (QTL), Fo-1.1, but resolution of this region has been poor due to low marker density. In this study, a combination of whole genome resequencing of bulked segregants (QTL-seq analysis) followed ...... hiện toàn bộ
A survey of cultivated heirloom tomato varieties identifies four new mutant alleles at the green-flesh locus
Molecular Breeding - Tập 24 - Trang 269-276 - 2009
Cornelius S. Barry, Priyanka Pandey
The process of crop domestication occurs through the selection and subsequent propagation of novel alleles that improve traits of interest. Cultivated tomato (Solanum lycopersicum), particularly heirloom varieties, exhibit a wide range of variation in fruit size, shape and color. The green-flesh mutant of tomato possesses a stay-green phenotype resulting in fruits that ripen to a red-brown color, ...... hiện toàn bộ
Accumulation of a thermostable endo-1,4-β-D-glucanase in the apoplast of Arabidopsis thaliana leaves
Molecular Breeding - - 2000
Matthew T. Ziegler, Steven R. Thomas, Kathleen J. Danna
Plant biomass, the most abundant renewable resource on earth, is a potential source of fermentable sugars for production of alternative transportation fuels and other chemicals. Bioconversion of plant biomass to fermentable glucose involves enzymatic hydrolysis of cellulose, a major polysaccharide constituent. Because commercially available microbial cellulases are prohibitively expensive for bioe...... hiện toàn bộ
Application of multiplex-ready PCR for fluorescence-based SSR genotyping in barley and wheat
Molecular Breeding - Tập 21 - Trang 271-281 - 2007
M. J. Hayden, T. M. Nguyen, A. Waterman, G. L. McMichael, K. J. Chalmers
Microsatellites (SSRs) are widely used in cereal research, and their use in marker assisted breeding has increased the speed and efficiency of germplasm improvement. Central to the application of SSRs for many purposes are methodologies enabling the low-cost acquisition of large quantities of genetic information for gene and genotype identification. In this study, multiplex-ready PCR was evaluated...... hiện toàn bộ
Constructing a genetic linkage map of Vitis aestivalis-derived “Norton” and its use in comparing Norton and Cynthiana
Molecular Breeding - Tập 37 - Trang 1-14 - 2017
Mia Hammers, Surya Sapkota, Li-Ling Chen, Chin-Feng Hwang
The first microsatellite-based linkage map for Vitis aestivalis was constructed using 183 progeny from the crosses of V. aestivalis-derived “Norton” and V. vinifera “Cabernet Sauvignon”. A total of 1157 simple sequence repeat (SSR) markers were tested, 859 were amplified via PCR and 413 were polymorphic for at least one parent. The map for Norton consisted of 376 markers and covered 1496.6 centimo...... hiện toàn bộ
Development of STS markers for Verticillium wilt resistance in cotton based on RGA–AFLP analysis
Molecular Breeding - Tập 34 - Trang 917-926 - 2014
Hui Fang, Huiping Zhou, Soum Sanogo, Jinfa Zhang
Verticillium wilt (VW) is one of the most destructive diseases of cotton that decreases yield and quality. Identification of resistance gene analogs (RGAs) can provide potential candidate gene markers for marker-assisted selection (MAS) for VW resistance and cloning of VW resistance genes. The objective of this study was to convert RGA-targeted RGA–AFLP (amplified fragment length polymorphism) mar...... hiện toàn bộ
Efficiency of linear selection index in predicting rice hybrid performance
Molecular Breeding - Tập 39 - Trang 1-13 - 2019
Xin Wang, Yang Xu, Pengchen Li, Mingyang Liu, Chenwu Xu, Zhongli Hu
Selection index (SI) theory has been applied to predict the net genetic merit, select parents for the next cycle, and maximize the selection response in plant breeding. However, up to now, SI has not been applied to predict unobserved hybrid performance. In hybrid breeding, it is impossible to test every cross, and accurate prediction can help breeders greatly reduce the experimental cost. Traditi...... hiện toàn bộ
Development of co-dominant KASP markers co-segregating with Ug99 effective stem rust resistance gene Sr26 in wheat
Molecular Breeding - Tập 38 - Trang 1-9 - 2018
Naeela Qureshi, Pakeerathan Kandiah, Mesfin Kebede Gessese, Vallence Nsabiyera, Vanessa Wells, Prashanth Babu, Debbie Wong, Matthew Hayden, Harbans Bariana, Urmil Bansal
Stem rust of wheat, caused by Puccinia graminis f. sp. tritici (Pgt), is a threat to global food security due to its ability to cause total crop failures. The Pgt race TTKSK (Ug99) and its derivatives detected in East Africa carry virulence for many resistance genes present in modern cultivars. However, stem rust resistance gene Sr26 remains effective to all races of Pgt worldwide. Sr26 is carried...... hiện toàn bộ
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