Molecular Breeding
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Elucidating the genetic background of the early-flowering transgenic genetic stock T1190 with a high-density SNP array
Molecular Breeding - - 2019
High glucosinolate broccoli: a delivery system for sulforaphane
Molecular Breeding - - 2006
The development of hybrid broccoli genotypes with enhanced levels of 4-methylsulphinylbutyl glucosinolate, the precursor of anticarcinogenic isothiocyanate sulforaphane (SF), by introgressing genomic segments from the wild ancestor Brassica villosa is described. We demonstrate that to obtain enhanced levels of either 3-methylsulphinylpropyl or 4-methylsulphinylbutyl glucosinolate it is necessary t......
Genetic mapping of Echinacea purpurea via individual pollen DNA fingerprinting
Molecular Breeding - Tập 21 - Trang 227-232 - 2007
The pollen genome of Echinacea purpurea ‘Magnus’ (purple coneflower) was amplified using a modified primer extension pre-amplification (PEP) procedures followed by AFLP (amplified fragment length polymorphism) analyses of individual pollen grains. Twenty-four AFLP primer pairs were identified that produced 104 scorable markers in eleven linkage groups. This is the first report on the production of......
An efficient and cost-effective approach for genic microsatellite marker-based large-scale trait association mapping: identification of candidate genes for seed weight in chickpea
Molecular Breeding - Tập 34 - Trang 241-265 - 2014
The large-scale validation and high-throughput genotyping of numerous informative genic microsatellite markers are required for association mapping to identify candidate genes for complex quantitative traits in chickpea. However, the screening and genotyping of such informative markers in individual genotypes/whole association panels for trait association mapping involves massive costs in terms of......
Diversity of Puroindoline genes and their association with kernel hardness in Chinese wheat cultivars and landraces
Molecular Breeding - Tập 39 - Trang 1-13 - 2019
Kernel hardness is a key trait that greatly affects the milling and baking qualities of common wheat. Kernel hardness is largely controlled by Pina and Pinb genes. Here, we investigate the allelic diversity of Pina and Pinb genes in a collection of 107 Chinese wheat cultivars and landraces. The kernel hardness values of these accessions were determined in two field seasons (2016/2017 and 2017/2018......
Molecular detection of a gene effective against powdery mildew in the wheat cultivar Liangxing 66
Molecular Breeding - Tập 30 - Trang 1737-1745 - 2012
Since it was commercialized in 2008, Liangxing 66 is one of the most widely grown cultivars of wheat (Triticum aestivum L.) in winter and facultative wheat-producing regions in northern China. This cultivar displays broad-spectrum resistance to isolates of powdery mildew. To identify the powdery mildew resistance gene in Liangxing 66, genetic analysis and molecular mapping were conducted using the......
Morphological and gene expression characterization of maf-1, a floral chili pepper mutant caused by a nonsense mutation in CaLFY
Molecular Breeding - Tập 42 - Trang 1-14 - 2022
Chili peppers are important as vegetables and ornamental crops, because of the variety of fruit shapes and colors. Understanding of flower and fruit development in Capsicum is limited compared with closely related Solanaceae crops such as tomato. This study reports a novel malformed fruit mutant named malformed fruit-1 (maf-1), which was isolated from an ethyl methanesulfonate–induced mutant popul......
Transgenic cucumber fruits that produce elevated level of an anti-aging superoxide dismutase
Molecular Breeding - Tập 11 - Trang 213-220 - 2003
Superoxide dismutase (SOD) plays an important role in cellular defense against oxidative stress in aerobic organisms. To generate cucumber (Cucumis sativus L.) fruits producing high yields of SOD for an anti-aging cosmetic material as a plant bioreactor, the CuZnSOD cDNA (mSOD1) from cassava was introduced into cucumber fruits by Agrobacterium-mediated transformation using the ascorbate oxidase pr......
Inheritance and gene mapping of spotted to non-spotted trait gene CmSp-1 in melon (Cucumis melo L. var. chinensis Pangalo)
Molecular Breeding - Tập 38 - Trang 1-9 - 2018
Melon (Cucumis melo L.) is one of the most popular and highly nutritious vegetable species within Cucurbitaceae. Because appearance is used as an important indicator of quality, the spotted to non-spotted trait associated with this product somewhat influences the buying habits of consumers. We tested a six-generation family to determine the inheritance and genetic basis of this trait. Genetic grou......
Commercial production of β-glucuronidase (GUS): a model system for the production of proteins in plants
Molecular Breeding - Tập 4 - Trang 301-312 - 1998
We have generated transgenic maize seed containing β-glucuronidase(GUS) for commercial production. While many other investigators have demonstrated the expression of GUS as a scoreable marker, this is one of the first cases where a detailed characterization of the transgenic plants and the protein were performed which are necessary to use this as a commercial source of GUS. The recombinant β-glucu......
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