This chapter focuses on the autoradiographic methods used for electron microscopy. The isotope of choice is tritium. It is the most easily adapted isotope because of its low average energy and consequently its high specific ionization. 3H emits a β-particle of 18 keV maximum energy and, therefore, gives excellent resolution and sensitivity. The nuclear emulsions used for autoradiography are compos...... hiện toàn bộ
This chapter discusses the procedures to increase virus yield from infected plants. Working efficiently with viruses requires both the production of virus in its host and its recovery in a form suitable for the purpose. Virus yield is based on work with tobacco mosaic virus (TMV). TMV is easy to produce and work with; is subject to every type of virus assay that has been used; and the results with...... hiện toàn bộ
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