Methods in Virology
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13 Autoradiographic Methods for Electron Microscopy
Tập 3 - Trang 617 - 1967
This chapter focuses on the autoradiographic methods used for electron microscopy. The isotope of choice is tritium. It is the most easily adapted isotope because of its low average energy and consequently its high specific ionization. 3H emits a β-particle of 18 keV maximum energy and, therefore, gives excellent resolution and sensitivity. The nuclear emulsions used for autoradiography are compos......
14 Procedures to Increase Virus Yield from Infected Plants
Tập 5 - Trang 451 - 1971
This chapter discusses the procedures to increase virus yield from infected plants. Working efficiently with viruses requires both the production of virus in its host and its recovery in a form suitable for the purpose. Virus yield is based on work with tobacco mosaic virus (TMV). TMV is easy to produce and work with; is subject to every type of virus assay that has been used; and the results with......
Use of Thin Sectioning for Visualization and Identification of Plant Viruses
Tập 8 - Trang 143-224 - 1984