Methods in Virology
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13 Autoradiographic Methods for Electron Microscopy
Tập 3 - Trang 617 - 1967
This chapter focuses on the autoradiographic methods used for electron
microscopy. The isotope of choice is tritium. It is the most easily adapted
isotope because of its low average energy and consequently its high specific
ionization. 3H emits a β-particle of 18 keV maximum energy and, therefore, gives
excellent resolution and sensitivity. The nuclear emulsions used for
autoradiography are compos...
Use of Thin Sectioning for Visualization and Identification of Plant Viruses
Tập 8 - Trang 143-224 - 1984
14 Procedures to Increase Virus Yield from Infected Plants
Tập 5 - Trang 451 - 1971
This chapter discusses the procedures to increase virus yield from infected
plants. Working efficiently with viruses requires both the production of virus
in its host and its recovery in a form suitable for the purpose. Virus yield is
based on work with tobacco mosaic virus (TMV). TMV is easy to produce and work
with; is subject to every type of virus assay that has been used; and the
results with...