Journal of Biological Engineering
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Surface modification of decellularized bovine carotid arteries with human vascular cells significantly reduces their thrombogenicity
Journal of Biological Engineering - Tập 15 - Trang 1-20 - 2021
Since autologous veins are unavailable when needed in more than 20% of cases in
vascular surgery, the production of personalized biological vascular grafts for
implantation has become crucial. Surface modification of decellularized
xenogeneic grafts with vascular cells to achieve physiological luminal coverage
and eventually thromboresistance is an important prerequisite for implantation.
However,...
Streamlining recombination-mediated genetic engineering by validating three neutral integration sites in Synechococcus sp. PCC 7002
Journal of Biological Engineering - Tập 11 - Trang 1-9 - 2017
Synechococcus sp. PCC 7002 (henceforth Synechococcus) is developing into a
powerful synthetic biology chassis. In order to streamline the integration of
genes into the Synechococcus chromosome, validation of neutral integration sites
with optimization of the DNA transformation protocol parameters is necessary.
Availability of BioBrick-compatible integration modules is desirable to further
simplify...
Development of a novel heterologous β-lactam-specific whole-cell biosensor in Bacillus subtilis
Journal of Biological Engineering - - 2020
Abstract Background Whole-cell biosensors are a powerful and easy-to-use
screening tool for the fast and sensitive detection of chemical compounds, such
as antibiotics. β-Lactams still represent one of the most important antibiotic
groups in therapeutic use. They interfere with late stages of the bacterial cell
wall biosynthesis and result in irreversible perturbations of cell division and
growth,...
Light regulation of secondary metabolism in fungi
Journal of Biological Engineering - Tập 17 - Trang 1-13 - 2023
Fungi have evolved unique metabolic regulation mechanisms for adapting to the
changing environments. One of the key features of fungal adaptation is the
production of secondary metabolites (SMs), which are essential for survival and
beneficial to the organism. Many of these SMs are produced in response to the
environmental cues, such as light. In all fungal species studied, the Velvet
complex tran...
RSM based optimization of nutritional conditions for cellulase mediated Saccharification by Bacillus cereus
Journal of Biological Engineering - Tập 12 - Trang 1-10 - 2018
Cellulases are enzyme which have potential applications in various industries.
Researchers are looking for potential cellulolytic bacterial strains for
industrial exploitation. In this investigation, cellulase production of Bacillus
cereus was explored while attacking poplar twigs. The bacterium was isolated
from the gut of freshwater fish, Labeo rohita and identified by 16S rRNA gene
sequencing t...
Discrimination between the effects of pulsed electrical stimulation and electrochemically conditioned medium on human osteoblasts
Journal of Biological Engineering - Tập 17 - Trang 1-18 - 2023
Electrical stimulation is used for enhanced bone fracture healing.
Electrochemical processes occur during the electrical stimulation at the
electrodes and influence cellular reactions. Our approach aimed to distinguish
between electrochemical and electric field effects on osteoblast-like MG-63
cells. We applied 20 Hz biphasic pulses via platinum electrodes for 2 h. The
electrical stimulation of th...
Advances in the application of Mxene nanoparticles in wound healing
Journal of Biological Engineering - Tập 17 Số 1
AbstractSkin is the largest organ of the human body. It plays a vital role as
the body’s first barrier: stopping chemical, radiological damage and microbial
invasion. The importance of skin to the human body can never be overstated.
Delayed wound healing after a skin injury has become a huge challenge in
healthcare. In some situations, this can have very serious and even
life-threatening effects o...
The LINC complex, mechanotransduction, and mesenchymal stem cell function and fate
Journal of Biological Engineering - Tập 13 - Trang 1-12 - 2019
Mesenchymal stem cells (MSCs) show tremendous promise as a cell source for
tissue engineering and regenerative medicine, and are understood to be
mechanosensitive to external mechanical environments. In recent years,
increasing evidence points to nuclear envelope proteins as a key player in
sensing and relaying mechanical signals in MSCs to modulate cellular form,
function, and differentiation. Of...
Stable, neuron-specific gene expression in the mouse brain
Journal of Biological Engineering - Tập 18 - Trang 1-16 - 2024
Gene delivery to, and expression in, the mouse brain is important for
understanding gene functions in brain development and disease, or testing gene
therapies. Here, we describe an approach to express a transgene in the mouse
brain in a cell-type-specific manner. We use stereotaxic injection of a
transgene-expressing adeno-associated virus into the mouse brain via the
intracerebroventricular route...
Formaldehyde formation in the glycine cleavage system and its use for an aldolase-based biosynthesis of 1,3-propanediol
Journal of Biological Engineering - - 2020
AbstractGlycine cleavage system (GCS) occupies a key position in one-carbon (C1)
metabolic pathway and receives great attention for the use of C1 carbons like
formate and CO2 via synthetic biology. In this work, we demonstrate that
formaldehyde exists as a substantial byproduct of the GCS reaction cycle. Three
causes are identified for its formation. First, the principal one is the
decomposition o...
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