Journal of Biological Engineering

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Surface modification of decellularized bovine carotid arteries with human vascular cells significantly reduces their thrombogenicity
Journal of Biological Engineering - Tập 15 - Trang 1-20 - 2021
Eriselda Keshi, Peter Tang, Marie Weinhart, Hannah Everwien, Simon Moosburner, Nicolai Seiffert, Michael Lommel, Ulrich Kertzscher, Brigitta Globke, Anja Reutzel-Selke, Benjamin Strücker, Johann Pratschke, Igor Maximillian Sauer, Nils Haep, Karl Herbert Hillebrandt
Since autologous veins are unavailable when needed in more than 20% of cases in vascular surgery, the production of personalized biological vascular grafts for implantation has become crucial. Surface modification of decellularized xenogeneic grafts with vascular cells to achieve physiological luminal coverage and eventually thromboresistance is an important prerequisite for implantation. However,...... hiện toàn bộ
Streamlining recombination-mediated genetic engineering by validating three neutral integration sites in Synechococcus sp. PCC 7002
Journal of Biological Engineering - Tập 11 - Trang 1-9 - 2017
Anne Ilse Maria Vogel, Rahmi Lale, Martin Frank Hohmann-Marriott
Synechococcus sp. PCC 7002 (henceforth Synechococcus) is developing into a powerful synthetic biology chassis. In order to streamline the integration of genes into the Synechococcus chromosome, validation of neutral integration sites with optimization of the DNA transformation protocol parameters is necessary. Availability of BioBrick-compatible integration modules is d...... hiện toàn bộ
Development of a novel heterologous β-lactam-specific whole-cell biosensor in Bacillus subtilis
Journal of Biological Engineering - - 2020
Nina Lautenschläger, Philipp F. Popp, Thorsten Mascher
Abstract Background Whole-cell biosensors are a powerful and easy-to-use screening tool for the fast and sensitive detection of chemical compounds, such as antibiotics. β-Lactams still represent one of the most important antibiotic groups in therapeutic use. They interfere with late stages of the bacterial cell wall biosynthesis a...... hiện toàn bộ
Light regulation of secondary metabolism in fungi
Journal of Biological Engineering - Tập 17 - Trang 1-13 - 2023
Wenbin Yu, Rongqiang Pei, Yufei Zhang, Yayi Tu, Bin He
Fungi have evolved unique metabolic regulation mechanisms for adapting to the changing environments. One of the key features of fungal adaptation is the production of secondary metabolites (SMs), which are essential for survival and beneficial to the organism. Many of these SMs are produced in response to the environmental cues, such as light. In all fungal species studied, the Velvet complex tran...... hiện toàn bộ
RSM based optimization of nutritional conditions for cellulase mediated Saccharification by Bacillus cereus
Journal of Biological Engineering - Tập 12 - Trang 1-10 - 2018
Fouzia Tabssum, Muhammad Irfan, Hafiz Abdullah Shakir, Javed Iqbal Qazi
Cellulases are enzyme which have potential applications in various industries. Researchers are looking for potential cellulolytic bacterial strains for industrial exploitation. In this investigation, cellulase production of Bacillus cereus was explored while attacking poplar twigs. The bacterium was isolated from the gut of freshwater fish, Labeo rohita and identified by 16S rRNA gene sequencing t...... hiện toàn bộ
Discrimination between the effects of pulsed electrical stimulation and electrochemically conditioned medium on human osteoblasts
Journal of Biological Engineering - Tập 17 - Trang 1-18 - 2023
Meike Bielfeldt, Kai Budde-Sagert, Nikolai Weis, Maren Buenning, Susanne Staehlke, Julius Zimmermann, Nils Arbeiter, Sahba Mobini, María Ujué González, Henrike Rebl, Adelinde Uhrmacher, Ursula van Rienen, Barbara Nebe
Electrical stimulation is used for enhanced bone fracture healing. Electrochemical processes occur during the electrical stimulation at the electrodes and influence cellular reactions. Our approach aimed to distinguish between electrochemical and electric field effects on osteoblast-like MG-63 cells. We applied 20 Hz biphasic pulses via platinum electrodes for 2 h. The electrical stimulation of th...... hiện toàn bộ
Advances in the application of Mxene nanoparticles in wound healing
Journal of Biological Engineering - Tập 17 Số 1
Chengzhi Liang, Jie He, Yuan Chen, Guoming Liu, Chengdong Zhang, Qi Zhang, Chen Fu, Yanling Hu
AbstractSkin is the largest organ of the human body. It plays a vital role as the body’s first barrier: stopping chemical, radiological damage and microbial invasion. The importance of skin to the human body can never be overstated. Delayed wound healing after a skin injury has become a huge challenge in healthcare. In some situations, this can have very serious an...... hiện toàn bộ
The LINC complex, mechanotransduction, and mesenchymal stem cell function and fate
Journal of Biological Engineering - Tập 13 - Trang 1-12 - 2019
Tasneem Bouzid, Eunju Kim, Brandon D. Riehl, Amir Monemian Esfahani, Jordan Rosenbohm, Ruiguo Yang, Bin Duan, Jung Yul Lim
Mesenchymal stem cells (MSCs) show tremendous promise as a cell source for tissue engineering and regenerative medicine, and are understood to be mechanosensitive to external mechanical environments. In recent years, increasing evidence points to nuclear envelope proteins as a key player in sensing and relaying mechanical signals in MSCs to modulate cellular form, function, and differentiation. Of...... hiện toàn bộ
Stable, neuron-specific gene expression in the mouse brain
Journal of Biological Engineering - Tập 18 - Trang 1-16 - 2024
Osama Ahmed, Kingsley M. Ekumi, Francesco V. Nardi, Gulimiheranmu Maisumu, Khaled Moussawi, Eric D. Lazartigues, Bo Liang, Abraam M. Yakoub
Gene delivery to, and expression in, the mouse brain is important for understanding gene functions in brain development and disease, or testing gene therapies. Here, we describe an approach to express a transgene in the mouse brain in a cell-type-specific manner. We use stereotaxic injection of a transgene-expressing adeno-associated virus into the mouse brain via the intracerebroventricular route...... hiện toàn bộ
Formaldehyde formation in the glycine cleavage system and its use for an aldolase-based biosynthesis of 1,3-propanediol
Journal of Biological Engineering - - 2020
Yingying Xu, Hao Meng, Jie Ren, An‐Ping Zeng
AbstractGlycine cleavage system (GCS) occupies a key position in one-carbon (C1) metabolic pathway and receives great attention for the use of C1 carbons like formate and CO2 via synthetic biology. In this work, we demonstrate that formaldehyde exists as a substantial byproduct of the GCS reaction cycle. Three causes are identified for its form...... hiện toàn bộ
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