Ex vivo evaluation of blood coagulation on endothelial glycocalyx-inspired surfaces using thromboelastographyIn vitro models - Tập 1 - Trang 59-71 - 2021
Yanyi Zang, Jessi R. Vlcek, Jamie Cuchiaro, Ketul C. Popat, Christine S. Olver, Matt J. Kipper, Melissa M. Reynolds
Present blood-contacting materials have not yet demonstrated to be effective in reducing blood coagulation without causing additional side effects clinically. We have developed an endothelial glycocalyx-inspired biomimetic surface that combines nanotopography, heparin presentation, and nitric oxide (NO)-releasing features. The resulting modified surfaces have already shown promise in reducing unfa...... hiện toàn bộ
Inflammatory bowel disease addressed by Caco-2 and monocyte-derived macrophages: an opportunity for an in vitro drug screening assayIn vitro models - Tập 1 - Trang 365-383 - 2022
Sabrina Schnur, Vanessa Wahl, Julia K. Metz, Jessica Gillmann, Fabian Hans, Katharina Rotermund, Ralf-Kilian Zäh, Dietmar A. Brück, Marc Schneider, Marius Hittinger
Inflammatory bowel disease (IBD) is a widespread disease, affecting a growing demographic. The treatment of chronic inflammation located in the GI-tract is dependent on the severity; therefore, the IBD treatment pyramid is commonly applied. Animal experimentation plays a key role for novel IBD drug development; nevertheless, it is ethically questionable and limited in its throughput. Reliable and ...... hiện toàn bộ
3D spheroid culture to examine adaptive therapy response in invading tumor cellsIn vitro models - Tập 1 - Trang 463-471 - 2023
Felix Weiss, Nader Atlasy, Vince van Reijmersdal, Henk Stunnenberg, Cornelia Hulsbergen-Veelken, Peter Friedl
3D in vitro culture models of cancer cells in extracellular matrix (ECM) have been developed to investigate drug targeting and resistance or, alternatively, mechanisms of invasion; however, models allowing analysis of shared pathways mediating invasion and therapy resistance are lacking. To evaluate therapy response associated with cancer cell invasion, we here used 3D invasion culture of tumor sp...... hiện toàn bộ
Application of a 3D hydrogel-based model to replace use of animals for passaging patient-derived xenograftsIn vitro models -
S. J. Jones, J. Ashworth, Marian L Meakin, Pamela Collier, C. Probert, Alison Ritchie, Catherine L.R. Merry, Anna Grabowska
Abstract
Purpose
This 3D in vitro cancer model for propagation of patient-derived cells, using a synthetic self-assembling peptide gel, allows the formation of a fully characterised, tailorable tumour microenvironment. Unlike many existing 3D cancer models, the peptide gel is inert, apart from molec...... hiện toàn bộ
Mô hình nuôi cấy để đánh giá độc tính thần kinh của phenylalanin trong bệnh phenylketonuria Dịch bởi AI In vitro models - Tập 1 - Trang 103-114 - 2022
Julian Kylies, Bianka Brunne, Gabriele M. Rune
Bệnh phenylketonuria (PKU) do một đột biến cụ thể của gen phenylalanine hydroxylase (PAH) gây ra. Sự thiếu hụt PAH dẫn đến mức phenylalanin cao (Phe), mức tyrosine thấp (Tyr), và giảm các chất truyền đạt thần kinh catecholamine. Phần lớn bệnh nhân PKU, nếu không được điều trị, sẽ phát triển chậm phát triển trí tuệ nghiêm trọng. Đóng góp cụ thể của mức Phe cao và Tyr thấp trong chậm phát triển trí ...... hiện toàn bộ
#phenylketonuria #phenylalanine #tyrosine #synapse #neurotoxicity #glial cells
Highlight: microfluidic devices for cancer metastasis studiesIn vitro models - Tập 1 - Trang 399-403 - 2022
Alice Scemama, Sophia Lunetto, Adrian Biddle
Whilst cancer is the single most researched disease by number of clinical trials, it remains a leading cause of death in middle- to high-income countries, with metastasis as the prime factor in cancer-associated mortality. The complex, multistep process of metastasis, in which cancer cells disseminate from the primary tumour and home to distant sites, is modulated by an extensive set of factors su...... hiện toàn bộ