In Vitro Cellular & Developmental Biology - Animal

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Isolation and characterization of embryonic ameloblast lineage cells derived from tooth buds of fetal miniature swine
In Vitro Cellular & Developmental Biology - Animal - Tập 52 Số 4 - Trang 445-453 - 2016
Taka Nakahara, Noriko Tominaga, Junko Toyomura, Toshiaki Tachibana, Yoshiaki Ide, Hiroshi Ishikawa
Establishment of astrocyte cell lines from sheep genetically susceptible to scrapie
In Vitro Cellular & Developmental Biology - Animal - Tập 36 - Trang 45-49 - 2000
D. Vilette, M. F. Madelaine, H. Laude
Primary cultures of the brain from sheep embryos were used to establish cell lines after transfection by the simian virus 40 (SV40) large T gene. Two of the lines (A15 and 4A6) displayed astroglial properties. They expressed the glial fibrillary acidic protein (GFAP), intermediate filament protein vimentin, and S-100 (beta-subunit) protein. While numerous rodent and human glial cell lines are avai...... hiện toàn bộ
DNA-Mediated gene transfection into primary cultures of adult mouse keratinocytes
In Vitro Cellular & Developmental Biology - Animal - - 1992
Natalie A. Betz, Ken J. Wolterman, John J. Reiners, Jill C. Pelling
Protein disulfide isomerase A3 might be involved in the regulation of 24-dehydrocholesterol reductase via vitamin D equilibrium in primary cortical neurons
In Vitro Cellular & Developmental Biology - Animal - Tập 57 - Trang 704-714 - 2021
Ulaş Yavuz, Merve Alaylıoğlu, Büşra Şengül, Spyridon N. Karras, Duygu Gezen-Ak, Erdinç Dursun
Vitamin D is a secosteroid hormone mediating its functions via vitamin D receptor (VDR) and an endoplasmic reticulum chaperone, protein disulfide isomerase A3 (PDIA3). From a physiological perspective, there is also a well-established association of cholesterol and vitamin D synthesis, since both share a common metabolic substrate, 7 dehydrocholesterol (7-DHC). Yet, the potential basic pathways, o...... hiện toàn bộ
Phenotypic differences between syrian hamster embryo cells cultured at pH 6.7 or 7.3
In Vitro Cellular & Developmental Biology - Animal - Tập 28 - Trang 635-642 - 1992
Robert J. Isfort, David B. Cody, Thomas N. Asquith, Robert A. LeBoeuf
We have investigated the molecular phenotypic differences between Syrian hamster embryo (SHE) cells cultured at pH 6.7 or 7.3. Multiple pH-sensitive phenotypic differences were noted including changes in cellular morphology, a unique charge differential in a major cellular protein, nine uniquely expressed proteins, two unique phosphoserine/threonine phosphoproteins, one unique phosphotyrosine phos...... hiện toàn bộ
Ciliary neurotrophic factor: a survival and differentiation inducer in human retinal progenitors
In Vitro Cellular & Developmental Biology - Animal - Tập 46 - Trang 635-646 - 2010
Kamla Dutt, Yang Cao, Ifeoma Ezeonu
Retinitis pigmentosa, age-related macular degeneration, and Parkinson’s disease remain major problems in the field of medicine. Some of the strategies being explored for treatment include replacement of damaged tissue by transplantation of healthy tissues or progenitor cells and delivery of neurotrophins to rescue degenerating tissue. One of the neurotrophins with promise is the ciliary neurotroph...... hiện toàn bộ
Quantum dots induce heat shock-related cytotoxicity at intracellular environment
In Vitro Cellular & Developmental Biology - Animal - Tập 50 - Trang 367-372 - 2013
Satoshi Migita, Alexandre Moquin, Hitomi Fujishiro, Seiichiro Himeno, Dusica Maysinger, Françoise M. Winnik, Akiyoshi Taniguchi
Quantum dots (QDs) are semiconductor nanocrystals with unique optical properties. Different proteins or polymers are commonly bound to their surfaces to improve biocompatibility. However, such surface modifications may not provide sufficient protection from cytotoxicity due to photodegradation and oxidative degradation. In this study, the cytotoxic effects of QDs, CdTe, and CdSe/ZnS were investiga...... hiện toàn bộ
The protective effect of silymarin on the carbon tetrachloride (CCl4)-induced liver injury in common carp (Cyprinus carpio)
In Vitro Cellular & Developmental Biology - Animal - Tập 49 - Trang 155-161 - 2013
Rui Jia, Liping Cao, Jinliang Du, Pao Xu, Galina Jeney, Guojun Yin
Silymarin, a mixture of bioactive flavonolignans from the milk thistle (Silybum marianum), is traditionally used in herbal medicine to defend against various hepatotoxic agents. The aim of the present study was to evaluate the protective effect of silymarin against carbon tetrachloride (CCl4)-induced liver injury in fish. Common carp, with an average initial weight of 17.0 ± 1.1 g, were fed diet c...... hiện toàn bộ
In vitro culture of blood cells from the colonial protochordateBotryllus schlosseri
In Vitro Cellular & Developmental Biology - Animal - Tập 29 - Trang 79-85 - 1993
Baruch Rinkevich, Claudette Rabinowitz
Primary cultures of circulatory blood cells from the colonial tunicateBotryllus schlosseri were cultivated in 96-well plates for up to 3 mo. in a medium based on Dulbecco’s modified Eagle’s medium, supplemented with salts to the botryllid ascidian hemolymph osmolarity, HEPES buffer,l-glutamin, fetal bovine serum, and antibiotics. Intercellular bridges between granular pigment cells were establishe...... hiện toàn bộ
Neuronal progenitor cells seeded in fibrin gel differentiate into ChAT-positive neuron
In Vitro Cellular & Developmental Biology - Animal - Tập 46 - Trang 738-745 - 2010
Jinbo Liu, Zhijian Zhang, Aihua Gong, Xudong Cao, Leimin Qian, Lirong Duan, Xianglan Sun, Xuefeng Bu
Previous studies indicate that neural progenitor cells seeded in fibrin can differentiate into the glia cells and neurons. However, whether fibrin gel can induce differentiation of neural progenitor cells of spinal cord into motoneurons remains to be elucidated. In this study, we prepared a fibrin-based hybrid gel incorporated with laminin and fibronectin using rat fresh plasma as a crosslinking a...... hiện toàn bộ
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