Conformational Transitions of Polynucleotides in Polymer MediaFEBS Journal - Tập 43 Số 2 - Trang 231-235 - 1974
Torvard C. Laurent, B. N. Preston, Birgit Carlsson
The melting temperature of three polynucleotides, poly(deoxyadenylic‐thymidylicf‐acid (double helix), calf‐thymus DNA and Staphylococcus aureus phage 80α DNA, increases in the presence of two neutral polymers, dextran and poly(ethyleneglycol). The results have been interpreted as a steric exclusion phenomenon. Two strands of polynucleotides in random coil conform...... hiện toàn bộ
The Covalently‐Bound Flavin of Hepatic Monoamine OxidaseFEBS Journal - Tập 24 Số 2 - Trang 328-331 - 1971
Wolfram H. Walker, Edna B. Kearney, R. Seng, Thomas P. Singer
In the previous paper in this series it was shown that a pure flavin pentapeptide isolated from hepatic monoamine oxidase contains 1 mole each of serine and tyrosine and 2 moles of glycine, as well as an additional amino acid which is covalently linked to the 8α carbon of riboflavin. This amino acid has been identified as cysteine and the linkage to the flavin as a thioether on the basis o...... hiện toàn bộ
The Covalently‐Bound Flavin of Hepatic Monoamine OxidaseFEBS Journal - Tập 24 Số 2 - Trang 321-327 - 1971
Edna B. Kearney, James I. Salach, Wolfram H. Walker, R. Seng, William C. Kenney, Eileen Zeszotek, Thomas P. Singer
A method has been elaborated for the large scale isolation of outer membranes of beef liver mitochondria. From these monoamine oxidase was partially purified and digested with trypsin and chymotrypsin to yield peptides containing riboflavin covalently linked to the peptide chain. A pure pentapeptide of riboflavin‐5′‐phosphate has been obtained by a series of chromatographic procedures. Edm...... hiện toàn bộ
Biochemical characterization of φX174‐protein‐E‐mediated lysis of Escherichia coliFEBS Journal - Tập 180 Số 2 - Trang 393-398 - 1989
Angela Witte, Werner Lubitz
Energetic and permeability properties of Escherichia coli cells were determined prior to and during lysis caused by expression of the cloned gene E of bacteriophage φX174. Before onset of cell lysis the transmembrane gradients for K+, Na+ or Mg2+/ions, the level of ATP and t...... hiện toàn bộ