European Biophysics Journal
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Bending undulations and elasticity of the erythrocyte membrane: effects of cell shape and membrane organization
European Biophysics Journal - Tập 18 - Trang 203-219 - 1990
The undulatory excitations (flickering) of human and camel erythrocytes were evaluated by employing the previously used flicker spectroscopy and by local measurements of the autocorrelation function K (t) of the cell thickness fluctuations using a dynamic image processing technique. By fitting theoretical and experimental flicker spectra relative values of the bending elastic modulus K
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Molecular dynamics simulations of unsaturated lipid bilayers: effects of varying the numbers of double bonds
European Biophysics Journal - - 2005
Effect of headgroup on the dipole potential of phospholipid vesicles
European Biophysics Journal - Tập 39 - Trang 103-110 - 2009
The dipole potentials, ψ
d, of phospholipid vesicles composed of pure dimyristoylphosphatidylcholine (DMPC) or vesicles in which 50 mol% of the DMPC was substituted by dimyristoylphosphatidylserine (DMPS), dimyristoylphosphatidylglycerol (DMPG), dimyristoylethanolamine (DMPE), dimyristoylphosphatidic acid (DMPA) or monomyristoylphosphatidylcholine (MMPC) were measured via a...... hiện toàn bộ
Adjustment of conformational flexibility of glyceraldehyde-3-phosphate dehydrogenase as a means of thermal adaptation and allosteric regulation
European Biophysics Journal - Tập 37 - Trang 1139-1144 - 2008
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) from Thermotoga maritima (TmGAPDH) is a thermostable enzyme (T
m = 102°C), which is fully active at temperatures near 80°C but has very low activity at room temperature. In search for an explanation of this behavior, we measured the conformational flexibility of the protein by hydrogen–deuterium exchange and compared the resu...... hiện toàn bộ
Orientation measurements on membrane systems made from lipopolysaccharides and free lipid A by FT-IR spectroscopy
European Biophysics Journal - Tập 16 - Trang 83-94 - 1988
Here we report on investigations into the orientational behaviour of hydrated membrane systems made from lipopolysaccharide and its lipid component, free lipid A, using Fourier-transform infrared spectroscopy and applying attenuated total reflectance. For the investigated lipopolysaccharides — extracted from mutants of Salmonella minnesota and Escherichia coli, and differing in the length of the p...... hiện toàn bộ
A pressure-polishing set-up to fabricate patch pipettes that seal on virtually any membrane, yielding low access resistance and efficient intracellular perfusion
European Biophysics Journal - Tập 40 - Trang 1215-1223 - 2011
When performing whole-cell configuration recordings, it is important to minimize series resistance to reduce the time constant of charging the cell membrane capacitance and to reduce error in membrane potential control. To this end, an existing method was improved by widening the patch pipette shank through the calibrated combination of heat and air pressure. The heat was produced by passing curre...... hiện toàn bộ
Molecular dynamics simulation of a phospholipid membrane
European Biophysics Journal - Tập 22 - Trang 423-436 - 1994
We present the results of molecular dynamics (MD) simulations of a phospholipid membrane in water, including full atomic detail. The goal of the simulations was twofold: first we wanted to set up a simulation system which is able to reproduce experimental results and can serve as a model membrane in future simulations. This goal being reached it is then further possible to gain insight in to those...... hiện toàn bộ
Elasticity and tumorigenic characteristics of cells in a monolayer after nanosecond pulsed electric field exposure
European Biophysics Journal - - 2017
Nanosecond pulsed electric fields (nsPEFs) applied to cells can induce different biological effects depending on pulse duration and field strength. One known process is the induction of apoptosis whereby nsPEFs are currently investigated as a novel cancer therapy. Another and probably related change is the breakdown of the cytoskeleton. We investigated the elasticity of rat liver epithelial cells ...... hiện toàn bộ
A neutral carrier-based liquid membrane microelectrode for divalent putrescine cations
European Biophysics Journal - Tập 28 - Trang 600-604 - 1999
A new ion-selective liquid membrane microelectrode, based on the neutral carrier 1,1′-bis(2,3-naphtho-18-crown-6), is described that shows the dependence of EMF on the activity of divalent putrescine cations a
Put, with the linear slope s
Put = 26 ± 3 mV/decade (mean ± SD, N = 18), in the range 10−4–10−1 M at 25 ± 1 °C. Values of potentiometric putrescine cation selectivity coefficients of logK
P...... hiện toàn bộ
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