European Biophysics Journal
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Forthcoming Meetings of Member Societies of the European Biophysical Societies' Association (EBSA)
European Biophysics Journal - Tập 22 - Trang 78-78 - 1993
Aminoethyl substitution enhances the self-assembly properties of an aminocellulose as a potential archaeological wood consolidant
European Biophysics Journal - Tập 49 - Trang 791-798 - 2020
The 6-deoxy-6-aminocelluloses—or “aminocelluloses”—are a class of synthetic natural cellulose derivatives which are mostly aqueous soluble and have excellent film-forming properties. Recent studies have connected these properties at the molecular level with protein-like self-associative behaviour for a range of aminocelluloses including a 6-deoxy-6-(ω-aminoethyl) aminocellulose AEA-1 with the asso......
Applications of electron paramagnetic resonance to studies of neurological disease
European Biophysics Journal - - 2008
Electron paramagnetic resonance spectroscopy (EPR) has the potential to give much detail on the structure of the paramagnetic transition ion coordination sites, principally of Cu2+, in a number of proteins associated with central nervous system diseases. Since these sites have been implicated in misfolding/mis-oligomerisation events associated with neurotoxic molecular species and/or the catalysis......
Solution NMR and CD spectroscopy of an intrinsically disordered, peripheral membrane protein: evaluation of aqueous and membrane-mimetic solvent conditions for studying the conformational adaptability of the 18.5 kDa isoform of myelin basic protein (MBP)
European Biophysics Journal - Tập 37 - Trang 1015-1029 - 2008
The stability and secondary structure propensity of recombinant murine 18.5 kDa myelin basic protein (rmMBP, 176 residues) was assessed using circular dichroic and nuclear magnetic resonance spectroscopy (1H–15N HSQC experiments) to determine the optimal sample conditions for further NMR studies (i.e., resonance assignments and protein-protein interactions). Six solvent conditions were selected ba......
Computational study of the transmembrane domain of the acetylcholine receptor
European Biophysics Journal - Tập 38 - Trang 961-970 - 2009
The nicotinic acetylcholine receptor (nAChR) is a ligand-gated ion channel protein whose transmembrane domain (TM-domain) is believed to be responsible for channel gating via a hydrophobic effect. In this work, we perform molecular dynamics and Brownian dynamics simulations to investigate the effect of transmembrane potential on the conformation and water occupancy of TM-domain, and the resulting ......
Proton relay system in the active site of maltodextrinphosphorylase via hydrogen bonds with large proton polarizability: an FT-IR difference spectroscopy study
European Biophysics Journal - Tập 28 - Trang 200-207 - 1999
Maltodextrinphosphorylase (MDP) was studied in the pH range 5.4–8.4 by Fourier transform infrared (FT-IR) spectroscopy. The pK
a value of the cofactor pyridoxalphosphate (PLP) was found between 6.5 and 7.0, which closely resembles the second pK
a of free PLP. FT-IR difference spectra of the binary complex of MDP+α-d-glucose-1-methylenephosphonate (Glc-1-MeP) minus native MDP were taken at pH 6.9.......
Helix-helix transitions in DNA: fibre X-ray study of the particular cases poly(dG-dC) · poly(dG-dC) and poly(dA) · 2poly(dT)
European Biophysics Journal - Tập 28 - Trang 574-582 - 1999
The helix-helix transitions which occur in poly(dG-dC) · poly(dG-dC) and in poly (dG-m5dC) · poly(dG-m5dC) are commonly assumed to be changes between the right-handed A- or B-DNA double helices and the left-handed Z-DNA structure. The mechanisms for such transconformations are highly improbable, especially when they are supposed to be active in long polynucleotide chains organised in semicrystall......
Is the Ca2+-ATPase from sarcoplasmic reticulum also a heat pump?
European Biophysics Journal - Tập 38 - Trang 59-67 - 2008
We calculate, using the first law of thermodynamics, the membrane heat fluxes during active transport of Ca2+ in the Ca2+-ATPase in leaky and intact vesicles, during ATP hydrolysis or synthesis conditions. The results show that the vesicle interior may cool down during hydrolysis and Ca2+-uptake, and heat up during ATP synthesis and Ca2+-efflux. The heat flux varies with the SERCA isoform. Electro......
Maximum-entropy decomposition of fluorescence correlation spectroscopy data: application to liposome–human serum albumin association
European Biophysics Journal - Tập 33 - Trang 59-67 - 2003
Fluorescence correlation spectroscopy was used to measure the diffusion behavior of a mixture of DMPC or DMPC/DMPG liposomes with human serum albumin (HSA) and mesoporphyrin (MP), which was used as the fluorescent label for liposomes and HSA as well. For decomposing the fluorescence intensity autocorrelation function (ACF) into components corresponding to a liposome population, HSA and MP, we used......
Analytical ultracentrifugation: still the gold standard that offers multiple solutions
European Biophysics Journal - Tập 49 - Trang 673-676 - 2020
Understanding the nature of macromolecules and their interactions in solution underpins many fields, including biology, chemistry and materials science. The 24th International Analytical Ultracentrifugation Workshop and Symposium (AUC2019, held in Christchurch, New Zealand, August 2019), brought together 77 international delegates to highlight recent developments in the field. There was a focus on......
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