Cytotechnology

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A reliable and economical method for gaining mouse embryonic fibroblasts capable of preparing feeder layers
Cytotechnology - Tập 68 - Trang 1603-1614 - 2015
Guangming Jiang, Xiaoju Wan, Ming Wang, Jianhua Zhou, Jian Pan, Baolong Wang
Mouse embryonic fibroblasts (MEFs) are widely used to prepare feeder layers for culturing embryonic stem cells (ESCs) or induced pluripotent stem cells (iPSCs) in vitro. Transportation lesions and exorbitant prices make the commercially obtained MEFs unsuitable for long term research. The aim of present study is to establish a method, which enables researchers to gain MEFs from mice and establish ...... hiện toàn bộ
Mechanisms for antidiabetic effect of gingerol in cultured cells and obese diabetic model mice
Cytotechnology - Tập 67 Số 4 - Trang 641-652 - 2015
Myoung Jin Son, Yutaka Miura, Kazumi Yagasaki
Effects of DNA on immunoglobulin production stimulating activity of alcohol dehydrogenase
Cytotechnology - Tập 31 - Trang 95-102 - 1999
Takeaki Okamoto, Hiroshi Furutani, Takeshi Sasaki, Takuya Sugahara
Alcohol dehydrogenase-I (ADH-I) derived from horse liver stimulated IgM production by human-human hybridoma, HB4C5 cells and lymphocytes. The IPSF activity of ADH-I was suppressed by coexistence of short DNA whose chain length is less than 200 base pairs (bp) and fibrous DNA in a dose-dependent manner. These DNA preparations completely inhibited the IPSF activity at the concentration of 250 μg/ml ...... hiện toàn bộ
Interleukin-4 suppresses the proliferation and alters the gene expression in enteroids
Cytotechnology - Tập 72 - Trang 479-488 - 2020
Yuki Saito, Ken Iwatsuki, Akihiko Inaba, Mika Sato, Miki Tadaishi, Makoto Shimizu, Kazuo Kobayashi-Hattori
Interleukin (IL)-4 is known as a cytokine mainly involved in allergy and inflammation, but recent studies have suggested that IL-4 plays a part in the differentiation process of various cells. Since the effect of IL-4 on intestinal epithelial cells, particularly cryptic cells including stem cells, is poorly understood, we investigated IL-4-induced changes in intestinal epithelial cells using mouse...... hiện toàn bộ
Productivity enhancement of recombinant protein in CHO cells via specific promoter activation by oncogenes
Cytotechnology - Tập 31 - Trang 103-109 - 1999
Yoshinori Katakura, Perry Seto, Takumi Miura, Hideya Ohashi, Kiichiro Teruya, Sanetaka Shirahata
To construct a recombinant protein highly producing cell lines, we have previously developed the Oncogene Activated Production (OAP) system by using BHK-21 cells. Here we verified the availability of the OAP system in CHO cells. We firstly generated ‘primed’ ras amplified CHO cells, ras clone I, by introducing human c-Ha-ras oncogene into CHO cells. This ras clone I enables quick and easy establis...... hiện toàn bộ
Differentiation of myoblasts and CNS cells grown either separately or as co-cultures on microcarriers
Cytotechnology - Tập 9 - Trang 107-115 - 1992
A. Shahar, S. Reuveny, M. Zhang, A. Espinosa de los Monteros, J. de Vellis, A. Shainberg
Dispersed neuronal and muscular elements from fetal or neonatal origin, can organize and mature in culture when grown on positively charged cylindrical microcarriers (MCS), to a stage which simulatein vivo maturation. Cells arrange themselves on the MCS to form aggregates which remain floating in the nutrient medium. In such a tridimensional organization, the neuronal tissue is capable of regenera...... hiện toàn bộ
In vitro culture conditions to study keratinocyte differentiation using the HaCaT cell line
Cytotechnology - Tập 54 - Trang 77-83 - 2007
Adeline F. Deyrieux, V. G. Wilson
In vitro models to study the process of keratinocyte differentiation have been hindered by the stringent culture requirements and limitations imposed by the inherent properties of the cells. Primary keratinocytes only have a finite life span, while transformed cell lines exhibit many phenotypic features not found in normal cells. The spontaneously immortalized HaCaT cell line has been a widely emp...... hiện toàn bộ
Rapid in vitro transformation system for liver epithelial cells by iron chelate, Fe-NTA
Cytotechnology - Tập 3 - Trang 149-156 - 1990
Masayasu Yamada, Michiyasu Awai, Tohru Okigaki
We have previously found toxic effects of iron chelate, Fe-NTA on cultured normal rat liver epithelial cells (RL34). In the present study, when RL34 cells were exposed to 50 μg/ml iron of Fe-NTA for 15 days, besides the expected cytolytic effects in most cells, the appearance of resistant cells was observed. The resistant cells showed drastic morphological transformation, grew in soft agar, and in...... hiện toàn bộ
Transient transfection of CHO cells using linear polyethylenimine is a simple and effective means of producing rainbow trout recombinant IFN-γ protein
Cytotechnology - Tập 67 - Trang 987-993 - 2014
Ronggai Li
A practical method was developed for the transient transfection of Chinese hamster ovary (CHO) cells with 25 kDa linear polyethylenimine (PEI) then optimal culture conditions determined for the production of rainbow trout (Oncorhynchus mykiss) IFN-γ recombinant protein. We found that culture temperature had a significant impact upon recombinant protein yield, with best results being obtained at 32...... hiện toàn bộ
Transfection of mouse cells with thymidine kinase gene of herpes simplex virus
Cytotechnology - Tập 3 - Trang 141-147 - 1990
Chee Gun Lee, Chan Gil Kim, Rock Namkung, Sang Eun Lee, Sang Dai Park
A mouse cell line (LP1-1) was established from the murine L cells deficient in thymidine kinase (L-M(TK −)) by prolonged selective culture on the hypoxanthine-aminopterine-thymidine (HAT) medium following transfection with the thymidine kinase gene of herpes simplex virus type-I (HSVTK). Southern blot analysis has shown that the viral TK gene was integrated into one of the chromosomal loci by a si...... hiện toàn bộ
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