Cytotechnology

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A reliable and economical method for gaining mouse embryonic fibroblasts capable of preparing feeder layers
Cytotechnology - Tập 68 - Trang 1603-1614 - 2015
Guangming Jiang, Xiaoju Wan, Ming Wang, Jianhua Zhou, Jian Pan, Baolong Wang
Mouse embryonic fibroblasts (MEFs) are widely used to prepare feeder layers for culturing embryonic stem cells (ESCs) or induced pluripotent stem cells (iPSCs) in vitro. Transportation lesions and exorbitant prices make the commercially obtained MEFs unsuitable for long term research. The aim of present study is to establish a method, which enables researchers to gain MEFs from mice and establish ...... hiện toàn bộ
Mechanisms for antidiabetic effect of gingerol in cultured cells and obese diabetic model mice
Cytotechnology - Tập 67 Số 4 - Trang 641-652 - 2015
Myoung Jin Son, Yutaka Miura, Kazumi Yagasaki
Productivity enhancement of recombinant protein in CHO cells via specific promoter activation by oncogenes
Cytotechnology - Tập 31 - Trang 103-109 - 1999
Yoshinori Katakura, Perry Seto, Takumi Miura, Hideya Ohashi, Kiichiro Teruya, Sanetaka Shirahata
To construct a recombinant protein highly producing cell lines, we have previously developed the Oncogene Activated Production (OAP) system by using BHK-21 cells. Here we verified the availability of the OAP system in CHO cells. We firstly generated ‘primed’ ras amplified CHO cells, ras clone I, by introducing human c-Ha-ras oncogene into CHO cells. This ras clone I enables quick and easy establis...... hiện toàn bộ
Differentiation of myoblasts and CNS cells grown either separately or as co-cultures on microcarriers
Cytotechnology - Tập 9 - Trang 107-115 - 1992
A. Shahar, S. Reuveny, M. Zhang, A. Espinosa de los Monteros, J. de Vellis, A. Shainberg
Dispersed neuronal and muscular elements from fetal or neonatal origin, can organize and mature in culture when grown on positively charged cylindrical microcarriers (MCS), to a stage which simulatein vivo maturation. Cells arrange themselves on the MCS to form aggregates which remain floating in the nutrient medium. In such a tridimensional organization, the neuronal tissue is capable of regenera...... hiện toàn bộ
In vitro culture conditions to study keratinocyte differentiation using the HaCaT cell line
Cytotechnology - Tập 54 - Trang 77-83 - 2007
Adeline F. Deyrieux, V. G. Wilson
In vitro models to study the process of keratinocyte differentiation have been hindered by the stringent culture requirements and limitations imposed by the inherent properties of the cells. Primary keratinocytes only have a finite life span, while transformed cell lines exhibit many phenotypic features not found in normal cells. The spontaneously immortalized HaCaT cell line has been a widely emp...... hiện toàn bộ
Transient transfection of CHO cells using linear polyethylenimine is a simple and effective means of producing rainbow trout recombinant IFN-γ protein
Cytotechnology - Tập 67 - Trang 987-993 - 2014
Ronggai Li
A practical method was developed for the transient transfection of Chinese hamster ovary (CHO) cells with 25 kDa linear polyethylenimine (PEI) then optimal culture conditions determined for the production of rainbow trout (Oncorhynchus mykiss) IFN-γ recombinant protein. We found that culture temperature had a significant impact upon recombinant protein yield, with best results being obtained at 32...... hiện toàn bộ
Transfection of mouse cells with thymidine kinase gene of herpes simplex virus
Cytotechnology - Tập 3 - Trang 141-147 - 1990
Chee Gun Lee, Chan Gil Kim, Rock Namkung, Sang Eun Lee, Sang Dai Park
A mouse cell line (LP1-1) was established from the murine L cells deficient in thymidine kinase (L-M(TK −)) by prolonged selective culture on the hypoxanthine-aminopterine-thymidine (HAT) medium following transfection with the thymidine kinase gene of herpes simplex virus type-I (HSVTK). Southern blot analysis has shown that the viral TK gene was integrated into one of the chromosomal loci by a si...... hiện toàn bộ
Anti-cancer drug-mediated increase in mitochondrial mass limits the application of metabolic viability-based MTT assay in cytotoxicity screening
Cytotechnology -
Abhishek Kumar, Yogesh Rai, Anant Narayan Bhatt
Metabolic and kinetic studies of hybridomas in exponentially fed-batch cultures using T-flasks
Cytotechnology - Tập 15 - Trang 73-86 - 1994
Ana E. Higareda, Lourival D. Possani, Octavio T. Ramírez
Exponentially fed-batch cultures (EFBC) of a murine hybridoma in T-flasks were explored as a simple alternative experimental tool to chemostats for the study of metabolism, growth and monoclonal antibody (MAb) production kinetics. EFBC were operated in the variable volume mode using an exponentially increasing and predetermined stepwise feeding profile of fresh complete medium. The dynamic and ste...... hiện toàn bộ
Explant organ culture: A review
Cytotechnology - Tập 7 - Trang 137-149 - 1991
James H. Resau, Kosaku Sakamoto, John R. Cottrell, Eric A. Hudson, Stephen J. Meltzer
Organ explant culture models offer several significant advantages for studies of patho-physiologic mechanisms like cell injury, secretion, differentiation and structure development. Organs or small explants/slices can be removed in vivo and maintained in vitro for extended periods of time if careful attention is paid to the media composition, substrate selection, and atmosphere. In the case of hum...... hiện toàn bộ
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