Cytotechnology

Công bố khoa học tiêu biểu

* Dữ liệu chỉ mang tính chất tham khảo

Sắp xếp:  
A reliable and economical method for gaining mouse embryonic fibroblasts capable of preparing feeder layers
Cytotechnology - Tập 68 - Trang 1603-1614 - 2015
Guangming Jiang, Xiaoju Wan, Ming Wang, Jianhua Zhou, Jian Pan, Baolong Wang
Mouse embryonic fibroblasts (MEFs) are widely used to prepare feeder layers for culturing embryonic stem cells (ESCs) or induced pluripotent stem cells (iPSCs) in vitro. Transportation lesions and exorbitant prices make the commercially obtained MEFs unsuitable for long term research. The aim of present study is to establish a method, which enables researchers to gain MEFs from mice and establish ...... hiện toàn bộ
Mechanisms for antidiabetic effect of gingerol in cultured cells and obese diabetic model mice
Cytotechnology - Tập 67 Số 4 - Trang 641-652 - 2015
Myoung Jin Son, Yutaka Miura, Kazumi Yagasaki
Effects of DNA on immunoglobulin production stimulating activity of alcohol dehydrogenase
Cytotechnology - Tập 31 - Trang 95-102 - 1999
Takeaki Okamoto, Hiroshi Furutani, Takeshi Sasaki, Takuya Sugahara
Alcohol dehydrogenase-I (ADH-I) derived from horse liver stimulated IgM production by human-human hybridoma, HB4C5 cells and lymphocytes. The IPSF activity of ADH-I was suppressed by coexistence of short DNA whose chain length is less than 200 base pairs (bp) and fibrous DNA in a dose-dependent manner. These DNA preparations completely inhibited the IPSF activity at the concentration of 250 μg/ml ...... hiện toàn bộ
Interleukin-4 suppresses the proliferation and alters the gene expression in enteroids
Cytotechnology - Tập 72 - Trang 479-488 - 2020
Yuki Saito, Ken Iwatsuki, Akihiko Inaba, Mika Sato, Miki Tadaishi, Makoto Shimizu, Kazuo Kobayashi-Hattori
Interleukin (IL)-4 is known as a cytokine mainly involved in allergy and inflammation, but recent studies have suggested that IL-4 plays a part in the differentiation process of various cells. Since the effect of IL-4 on intestinal epithelial cells, particularly cryptic cells including stem cells, is poorly understood, we investigated IL-4-induced changes in intestinal epithelial cells using mouse...... hiện toàn bộ
Productivity enhancement of recombinant protein in CHO cells via specific promoter activation by oncogenes
Cytotechnology - Tập 31 - Trang 103-109 - 1999
Yoshinori Katakura, Perry Seto, Takumi Miura, Hideya Ohashi, Kiichiro Teruya, Sanetaka Shirahata
To construct a recombinant protein highly producing cell lines, we have previously developed the Oncogene Activated Production (OAP) system by using BHK-21 cells. Here we verified the availability of the OAP system in CHO cells. We firstly generated ‘primed’ ras amplified CHO cells, ras clone I, by introducing human c-Ha-ras oncogene into CHO cells. This ras clone I enables quick and easy establis...... hiện toàn bộ
The static magnetic field accelerates the osteogenic differentiation and mineralization of dental pulp cells
Cytotechnology - Tập 62 - Trang 143-155 - 2010
Shan-hui Hsu, Jui-Chih Chang
Dental pulp cells (DPCs) can differentiate into osteoblasts and are deemed a promising cell source for bone regeneration. Static magnetic field (SMF) stimulates osteoblast differentiation but the effect in DPCs remains unknown. The aim of this study was to investigate the effect of SMF exposure on the osteogenic differentiation and mineralization of rat DPCs in vitro. Cells were continuously expos...... hiện toàn bộ
Development and validation of immortalized bovine mammary epithelial cell line as an in vitro model for the study of mammary gland functions
Cytotechnology - Tập 70 - Trang 67-82 - 2017
Ji-Xia Li, Abdelrahman Said, Xiu-Guo Ge, Wenxiu Wang, Yong Zhang, Tianming Jin
This study aimed to develop a bovine mammary epithelial (BME) cell line model, which provides a possibility to determine functional properties of the bovine mammary gland. The primary cell culture was derived from bovine mammary gland tissues and processed enzymatically to obtain cell colonies with epithelial-like morphology. The cultures of BME cells were purified and optimally cultured at 37 °C ...... hiện toàn bộ
Differentiation of myoblasts and CNS cells grown either separately or as co-cultures on microcarriers
Cytotechnology - Tập 9 - Trang 107-115 - 1992
A. Shahar, S. Reuveny, M. Zhang, A. Espinosa de los Monteros, J. de Vellis, A. Shainberg
Dispersed neuronal and muscular elements from fetal or neonatal origin, can organize and mature in culture when grown on positively charged cylindrical microcarriers (MCS), to a stage which simulatein vivo maturation. Cells arrange themselves on the MCS to form aggregates which remain floating in the nutrient medium. In such a tridimensional organization, the neuronal tissue is capable of regenera...... hiện toàn bộ
Replating of bioreactor expanded human bone marrow results in extended growth of primitive and mature cells
Cytotechnology - - 1995
Bernhard Ø. Palsson, Deok-Kun Oh, Manfred R. Koller
The determination of cellular viability of hybridoma cells in microtitre plates: A colorimetric assay based on neutral red
Cytotechnology - Tập 13 - Trang 227-232 - 1993
K. Modha, J. P. Whiteside, R. E. Spier
A colorimetric assay utilising Neutral Red (C.I. 50040), a nuclear stain, was developed to determine the cellular viability of hybridoma cells in microtitre plates. A linear correlation (r=0.99) was found to exist between the uptake of Neutral Red by viable cells and the viable cell count determined by Trypan blue exclusion test. The linearity stretched over the range of cell concentrations normal...... hiện toàn bộ
Tổng số: 2,059   
  • 1
  • 2
  • 3
  • 4
  • 5
  • 6
  • 10