Cytotechnology
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The cold-shock response in mammalian cells: investigating the HeLa cell cold-shock proteome
Cytotechnology - Tập 53 - Trang 47-53 - 2007
In recent years there have been a number of reports that suggest the sub-physiological (<37 °C) temperature in vitro culturing of mammalian cells can result in enhanced heterologous protein production. Despite these reports, the mechanisms by which mammalian cells respond to such conditions are largely unknown. We therefore set out to use a model in vitro culture HeLa cell system to begin investig...... hiện toàn bộ
Isolation and characterization of hair follicle stem cells from Arbas Cashmere goat
Cytotechnology - Tập 68 - Trang 2579-2588 - 2016
In this study, highly purified hair follicle stem cells from Arbas Cashmere goat (gHFSCs) were isolated using enzyme digestion and adhesion to type IV collagen. The biological characteristics of the gHFSCs were identified by morphological observation, growth curve, markers assay and differentiation in vitro. The gHFSCs were in small cell size with typical cobblestone morphology, good adhesion and ...... hiện toàn bộ
Microcarrier cultivation of bovine aortic endothelial cells in spinner vessels and a membrane stirred bioreactor
Cytotechnology - Tập 18 - Trang 193-206 - 1995
Primary bovine aortic endothelial cells were cultivated in serum supplemented medium without any additional growth factors. The anchorage dependent cells were propagated on Dormacell® microcarriers with covalently bound dimeric DEAE-groups at the surface of the dextrane beads. Cultivations were performed in 200 ml spinner cultures containing 1 g l−1 to 3 g l−1 of microcarriers. Out of five types o...... hiện toàn bộ
Cyanidin-3-glucoside enhances mitochondrial function and biogenesis in a human hepatocyte cell line
Cytotechnology - Tập 70 - Trang 1519-1528 - 2018
Mitochondrial dysfunction has been identified as one of the primary factors contributing to liver diseases. Pathways that control mitochondrial biogenesis are potential therapeutic targets for the amelioration of hepatocyte dysfunction and liver disease. Research on natural pharmacological agents that ameliorate liver diseases has intensified over the last two decades. Cyanidin-3-glucoside (Cy3g),...... hiện toàn bộ
Analysis of the mechanism of the tight-junctional permeability increase by capsaicin treatment on the intestinal Caco-2 cells
Cytotechnology - Tập 40 - Trang 93-98 - 2002
In a previous experiment (Isoda et al., 2001), we showed that the tight-junctional (TJ) permeability increase in Caco-2 cells during capsaicin exposure was through binding of the capsaicin molecule to a capsaicin receptor-like protein. In the present study, we examined how actin, which modulates TJ permeability, is influenced by capsaicin. We showed that after treatment of the Caco-2 cells with ca...... hiện toàn bộ
Constructive improvement of the ultrasonic separation device ADI 1015
Cytotechnology - Tập 34 - Trang 175-179 - 2000
The use of the ultrasonic separation deviceis a very important step in the direction forimproving animal cell bioreactor cultures. However,the normal construction of the ultrasonic separationdevice ADI 1015 has an inherent disadvantage inpumping the cell suspension continuously through thedevice by using a peristaltic pump. The cells aretaken out of the reactor and are transported to theside inlet...... hiện toàn bộ
Improved cosmetic activity by optimizing the Lithospermum erythrorhizon extraction process
Cytotechnology - Tập 67 - Trang 51-65 - 2013
This study was conducted to expand the use of Lithospermum erythrorhizon, which is a good source of natural dye, in skin whitening and immune activation cosmetics. The goal was to provide cosmeceutical data about the extraction yield and shikonin contents of this plant by optimizing the ultrasonic extraction and high pressure extraction conditions. Under optimal extraction conditions, which consis...... hiện toàn bộ
Inter embryonic (homo- or hetero-sexual) transfer of primordial germ cells between chicken embryos
Cytotechnology - Tập 33 - Trang 101-108 - 2000
Chicken primordial germ cells (PGCs) collected from thecirculating blood in embryonic vessels at stage 13–15 were inter-embryonically, homo- or hetero-sexually,transferred to the blood vessels of recipient embryosat the same stage of development. Approximately 30%of the embryos treated with hetero-sexual transfer of PGCs had abnormal gonads, showing ovotestis likeorgans. In this case, some of thes...... hiện toàn bộ
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