Clinical Chemistry
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Genotyping of Single-Nucleotide Polymorphisms by High-Resolution Melting of Small AmpliconsAbstract Background: High-resolution melting of PCR amplicons with the DNA dye LCGreen™ I was recently introduced as a homogeneous, closed-tube method of genotyping that does not require probes or real-time PCR. We adapted this system to genotype single-nucleotide polymorphisms (SNPs) after rapid-cycle PCR (12 min) of small amplicons (≤50 bp). Method... ...
Clinical Chemistry - Tập 50 Số 7 - Trang 1156-1164 - 2004
High-Resolution DNA Melting Analysis for Simultaneous Mutation Scanning and Genotyping in SolutionAbstract
Background: High-resolution DNA melting analysis with saturation dyes for either mutation scanning of PCR products or genotyping with unlabeled probes has been reported. However, simultaneous PCR product scanning and probe genotyping in the same reaction has not been described.
Methods: Asymmetric PCR was perf... ...
Clinical Chemistry - Tập 51 Số 10 - Trang 1770-1777 - 2005
Validating a Rapid Method for Detecting Common Polymorphisms in the APOA5 Gene by Melting Curve Analysis Using LightTyper
Clinical Chemistry - Tập 51 Số 7 - Trang 1279-1282 - 2005
Outliers in clinical chemistry quality-control schemes.Abstract
Results submitted to large-scale quality-control schemes are commonly judged against the mean and standard deviation (SD) of the results from other laboratories. It is desirable to ignore outlying values in estimating this mean and standard deviation, and results more than 2.5 or 3 SD from the mean are commonly rejected. I show that an outli... ...
Clinical Chemistry - Tập 25 Số 5 - Trang 675-677 - 1979
Standardization in peptide hormone immunoassays: principle and practice.
Clinical Chemistry - Tập 22 Số 7 - Trang 957-963 - 1976
Robust nonradioactive oligonucleotide ligation assay to detect a common point mutation in the CYP2D6 gene causing abnormal drug metabolismAbstract
A new nonradioactive oligonucleotide ligation assay for the detection of a common point mutation in the CYP2D6 gene is presented. The assay takes advantage of simultaneous time-resolved fluorescence measurements of lanthanide-labeled probes and the specificity of T4-DNA ligase in combination with the polymerase chain reaction. This strategy ... ...
Clinical Chemistry - Tập 41 Số 3 - Trang 413-418 - 1995
High-Resolution Genotyping by Amplicon Melting Analysis Using LCGreenAbstract Background: High-resolution amplicon melting analysis was recently introduced as a closed-tube method for genotyping and mutation scanning (Gundry et al. Clin Chem 2003;49:396–406). The technique required a fluorescently labeled primer and was limited to the detection of mutations residing in the melting domain of the labeled primer. Our aim was to develop ... ...
Clinical Chemistry - Tập 49 Số 6 - Trang 853-860 - 2003
Serum catalase activity for detection of hemolytic diseases.
Clinical Chemistry - Tập 29 Số 4 - Trang 741-743 - 1983
Serum catalase enzyme activity in acute pancreatitis.
Clinical Chemistry - Tập 28 Số 9 - Trang 1999-2000 - 1982
Quang Phổ MicroRNA trong 12 Dịch Cơ thể Dịch bởi AI
ĐẶT VẤN ĐỀ
MicroRNA (miRNA) là các RNA nhỏ không mã hóa đóng vai trò quan trọng trong việc điều chỉnh nhiều quá trình sinh học thông qua tương tác với RNA thông tin của tế bào. Các miRNA ngoại bào trong huyết thanh, huyết tương, nước bọt và nước tiểu gần đây đã được chứng minh có liên quan đến nhiều tình trạng bệnh lý,... ...
Clinical Chemistry - Tập 56 Số 11 - Trang 1733-1741 - 2010
Tổng số: 379
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