High-Resolution Genotyping by Amplicon Melting Analysis Using LCGreenClinical Chemistry - Tập 49 Số 6 - Trang 853-860 - 2003
Carl T. Wittwer, G. H. Reed, Cameron N. Gundry, Joshua G Vandersteen, Rob Pryor
AbstractBackground: High-resolution amplicon melting analysis was recently introduced as a closed-tube method for genotyping and mutation scanning (Gundry et al. Clin Chem 2003;49:396–406). The technique required a fluorescently labeled primer and was limited to the detection of mutations residing in the melting domain of the labeled primer. Our aim was to develop ...... hiện toàn bộ
Quantitative Identification of Urinary CalculiClinical Chemistry - Tập 1 Số 4 - Trang 241-248 - 1955
Reid H. Leonard, Arthur J. Butt
Abstract
Quantitative determination of calcium, phosphorus, and oxalic acid, assisted by occasional determination of ammonia nitrogen and insoluble nitrogen when indicated by qualitative tests, provides a means of computing the component substances of calculi. The determinations may be performed by slight modifications of the methods in use in the ro...... hiện toàn bộ
Simplified isoelectric focusing/immunoblotting determination of apoprotein E phenotypeClinical Chemistry - Tập 40 Số 1 - Trang 11-13 - 1994
Shinkuro Kataoka, M Paidi, Barbara V. Howard
Abstract
We developed a rapid, accurate method for phenotyping apoprotein E that can be used for large-scale population studies. In this method, adapted from the method of Kamboh et al. (J Lipid Res 1988;29:1535-43), 10-microL plasma samples are incubated with dithiothreitol and Tween-20 for 15 min and then applied to 5% polyacrylamide gels containin...... hiện toàn bộ
Serum Creatinine as an Index of Renal Function: New Insights into Old ConceptsClinical Chemistry - Tập 38 Số 10 - Trang 1933-1953 - 1992
Ronald D. Perrone, Nicolaos E. Madias, Andrew S. Levey
Abstract
The serum creatinine concentration is widely interpreted as a measure of the glomerular filtration rate (GFR) and is used as an index of renal function in clinical practice. Glomerular filtration of creatinine, however, is only one of the variables that determines its concentration in serum. Alterations in renal handling and metabolism of cr...... hiện toàn bộ
Radioreceptor Assay for Quantifying FK-506 Immunosuppressant in Whole BloodClinical Chemistry - Tập 38 Số 7 - Trang 1307-1310 - 1992
Jayasimha N. Murthy, Yongjun Chen, V Warty, R. Venkataramanan, James G. Donnelly, A Zeevi, Steven J. Soldin
Abstract
We describe a quantitative radioreceptor assay (RRA) for quantifying FK-506 in whole blood. FK-506 extracted from whole blood with a cyclohexyl-sorbent column competes with [3H]dihydro-FK-506 for binding to a partially purified preparation of FK-506 binding protein (FK-BP). Free and protein-bound FK-506 are separated on LH 20 Sephadex chroma...... hiện toàn bộ
Lack of Concordance between the 75-g and 100-g Glucose Load Tests for the Diagnosis of Gestational Diabetes MellitusClinical Chemistry - Tập 52 Số 9 - Trang 1679-1684 - 2006
Giorgio Mello, Elena Parretti, Agostino Ognibene, Riccardo Cioni, Tondi Filippo, Paola Pezzati, Monica Pratesi, Gianfranco Scarselli, Gianni Messeri
Abstract
Background: Gestational diabetes mellitus (GDM) is common and can have a substantial impact on fetal growth, birth weight, and morbidity. The American Diabetes Association recommends GDM testing with either a 3-h, 100-g glucose load (100g) (criteria according to Am J Obstet Gynecol 1982;144:768–73) or a 2-h, 75-g glucose load (75g). We inves...... hiện toàn bộ
Catecholamines in depression: an updateClinical Chemistry - Tập 40 Số 2 - Trang 279-287 - 1994
Michael Hüll, Husseini K. Manji
Abstract
Despite extensive research, the biochemical abnormalities underlying the predisposition to and the pathogenesis of affective disorders remain to be clearly established. Efforts to study norepinephrine (NE) output and function have utilized biochemical assays, neuroendocrine challenge strategies, and measures of peripheral blood cell receptor...... hiện toàn bộ