Influence of Statistical Method Used on the Resulting Estimate of Normal RangeClinical Chemistry - Tập 17 Số 4 - Trang 275-284 - 1971
Allen H. Reed, Richard J. Henry, William B. Mason
Abstract
The choice of statistical method can greatly influence the calculated normal range apart from any biological or chemical considerations. Nonparametric normal range estimates are, for practical purposes, as accurate as estimates that assume the distribution of data to be gaussian or loggaussian when the distribution assumed is true. When data...... hiện toàn bộ
Rapid Determination of Transferrin Isoforms by Immunoaffinity Liquid Chromatography and Electrospray Mass SpectrometryClinical Chemistry - Tập 47 Số 3 - Trang 513-518 - 2001
Jean M. Lacey, H. Robert Bergen, Mark J. Magera, Jake Y. Chen, John F. O’Brien
AbstractBackground: Congenital disorders of glycosylation (CDG) are autosomal recessive disorders that produce increased serum carbohydrate-deficient transferrin (CDT) isoforms. Methods to resolve CDT from fully glycosylated transferrin (Trf) have been based on a neutral shift in the isoelectric focusing (IEF) pattern or on a reduction in the negative charge, allow...... hiện toàn bộ
Sensitivity and Specificity of Single-Nucleotide Polymorphism Scanning by High-Resolution Melting AnalysisClinical Chemistry - Tập 50 Số 10 - Trang 1748-1754 - 2004
G. H. Reed, Carl T. Wittwer
Abstract
Background: Screening for heterozygous sequence changes in PCR products, also known as “mutation scanning”, is an important tool for genetic research and clinical applications. Conventional methods require a separation step.
Methods: We evaluated the sensitivity and specificity of homogeneous scanning, using a...... hiện toàn bộ
High-Resolution DNA Melting Analysis for Simultaneous Mutation Scanning and Genotyping in SolutionClinical Chemistry - Tập 51 Số 10 - Trang 1770-1777 - 2005
Luming Zhou, Lesi Wang, Robert Palais, Robert J. Pryor, Carl T. Wittwer
Abstract
Background: High-resolution DNA melting analysis with saturation dyes for either mutation scanning of PCR products or genotyping with unlabeled probes has been reported. However, simultaneous PCR product scanning and probe genotyping in the same reaction has not been described.
Methods: Asymmetric PCR was perf...... hiện toàn bộ
Genotyping of Single-Nucleotide Polymorphisms by High-Resolution Melting of Small AmpliconsClinical Chemistry - Tập 50 Số 7 - Trang 1156-1164 - 2004
Michael Liew, Robert J. Pryor, Robert Palais, Cindy Meadows, Maria Erali, Elaine Lyon, Carl T. Wittwer
AbstractBackground: High-resolution melting of PCR amplicons with the DNA dye LCGreen™ I was recently introduced as a homogeneous, closed-tube method of genotyping that does not require probes or real-time PCR. We adapted this system to genotype single-nucleotide polymorphisms (SNPs) after rapid-cycle PCR (12 min) of small amplicons (≤50 bp).Method...... hiện toàn bộ
Carboxyhemoglobin as measured by gas chromatography and with the IL 282 and 482 CO-Oximeters.Clinical Chemistry - Tập 34 Số 12 - Trang 2562-2566 - 1988
H J Vreman, John J. Mahoney, A L Van Kessel, David K. Stevenson
Abstract
We measured the concentration of carboxyhemoglobin (HbCO) in original and CO-tonometered blood samples from 53 intensive-care patients with IL 282 and IL 482 CO-Oximeters and by gas chromatography (GC), finding very strong correlations among the three methods for HbCO concentrations greater than 2.5%. For concentrations within the normal ref...... hiện toàn bộ