Chromosoma

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Scanning electron microscope analysis of structural changes and aberrations in human chromosomes associated with the inhibition and reversal of inhibition of ultraviolet light induced DNA repair
Chromosoma - Tập 96 - Trang 39-44 - 1987
A. M. Mullinger, R. T. Johnson
Metaphase chromosomes appear decondensed in preparations from mitotic cells that have been irradiated with ultraviolet light (UV) and incubated with inhibitors of DNA synthesis; under these conditions DNA repair is inhibited and both single and double strand DNA breaks accumulate. After reversal of the inhibition chromosomes are condensed, but are often damaged. In this paper we show by scanning e... hiện toàn bộ
Localization and characteristics of DNA underreplication zone in the 75C region of intercalary heterochromatin in Drosophila melanogaster polytene chromosomes
Chromosoma - - 2009
Natalya G. Andreyenkova, E. B. Kokoza, V. F. Semeshin, E. S. Belyaeva, С. А. Демаков, Alexey V. Pindyurin, Evgeniya N. Andreyeva, E. I. Volkova, И. Ф. Жимулев
Localization of actin-related sequences by in situ hybridization to R-banded human chromosomes
Chromosoma - Tập 89 - Trang 163-167 - 1984
A. Bernheim, R. Berger, P. Szabo
Using a cytoplasmic actin cDNA probe we have localized a number of actin sequences in the human genome using a novel in situ hybridization technique. Metaphase chromosomes treated to produce R-bands were directly annealed with 125I-labeled actin probe. Under these conditions many regions of the genome were apparently denatured enough to be capable of hybridizing with the probe. Most of the actin s... hiện toàn bộ
Microdissection and sequence analysis of pericentric heterochromatin from the Drosophila melanogaster mutant Suppressor of Underreplication
Chromosoma - Tập 111 - Trang 114-125 - 2002
Yuri M. Moshkin, Stepan N. Belyakin, Nikolay B. Rubtsov, Elena B. Kokoza, Artem A. Alekseyenko, Elena I. Volkova, Elena S. Belyaeva, Igor V. Makunin, Pierre Spierer, Igor F. Zhimulev
In the Suppressor of Underreplication (SuUR) mutant strain of Drosophila melanogaster, the heterochromatin of polytene chromosomes is not underreplicated and, as a consequence, a number of β-heterochromatic regions acquire a banded structure. The chromocenter does not form in these polytene chromosomes, and heterochromatic regions, normally part of the chromocenter, become accessible to cytologica... hiện toàn bộ
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Chromosoma - Tập 14 - Trang 676-692 - 1963
3H-Uridine labelling patterns in the Drosophila melanogaster salivary gland chromosomes X, 2R and 3L
Chromosoma - Tập 49 - Trang 219-231 - 1975
I. F. Zhimulev, E. S. Belyaeva
RNA-synthesizing regions in the polytene chromosomes of D. melanogaster were located; the level of 3H-uridine incorporation in each of these regions was determined. — It was found that the number of RNA-synthesizing regions is much larger than the number of puffs: almost all the chromosome regions are labelled except dense bands. In most cases, “non-puffed” regions label 10 to even 100 times weake... hiện toàn bộ
The process of a Y-loss event in an XO/XO mammal, the Ryukyu spiny rat
Chromosoma - Tập 119 - Trang 519-526 - 2010
Asato Kuroiwa, Yasuko Ishiguchi, Fumio Yamada, Abe Shintaro, Yoichi Matsuda
The Ryukyu spiny rat, Tokudaia osimensis, has an XO/XO sex chromosome constitution, lacking a Y chromosome and the mammalian sex-determining gene SRY. To investigate the Y-loss event, we traced three proto-Y-linked genes, RBMY1A1, EIF2S3Y, and KDM5D, in the genome. The original Y-linked RBMY1A1 was lost as well as SRY, and the remaining RBMY1A1 was a processed pseudogene on autosome. In contrast, ... hiện toàn bộ
The labeling efficiency of human telomeres is increased by double-strand PRINS
Chromosoma - Tập 113 - Trang 204-209 - 2004
Ju Yan, Bing-Zhen Chen, Eric F. Bouchard, Régen Drouin
Telomeres are composed of tandem repeated sequences, TTAGGG, that can be detected either by fluorescence in situ hybridization (FISH), more efficiently by using a peptide nucleic acid (PNA) probe, or by the primed in situ (PRINS) technique. However, the efficiency of human telomere labeling using PRINS is somewhat lower than the efficiency using PNA-FISH. To solve this problem, we developed a doub... hiện toàn bộ
Systematic gene targeting on the X chromosome of Drosophila melanogaster
Chromosoma - - 2004
Nicole Beinert, Meike Werner, G. H. C. Dowe, Ho‐Ryun Chung, Herbert Jäckle, Ulrich Schäfer
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