Chromosoma
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Localization and characteristics of DNA underreplication zone in the 75C region of intercalary heterochromatin in Drosophila melanogaster polytene chromosomes
Chromosoma - - 2009
FISH analysis of the telomere sequences of bulldog ants (Myrmecia: Formicidae)
Chromosoma - Tập 104 - Trang 14-18 - 1995
Chromosomes from several species of ants from the genus Myrmecia were hybridized with deoxyoligomer probes of either (T2AG2)7, the putative insect telomere repeat sequence, or (T2AG3)7, the vertebrate telomere repeat sequence. While both sequence hibridized over a range of stringency conditions, (T2AG2)n was clearly the predominant sequence at the termini of the Myrmecia chromosomes. No interstiti......
Differential Giemsa staining of heterochromatic B-chromosomes in Myrmeleotettix maculatus (Thunb.) (Orthoptera: Acrididae)
Chromosoma - Tập 40 - Trang 167-172 - 1972
A modified Giemsa staining technique has been used to investigate the karyotype of the grasshopper Myrmeleotettix maculatus, since this stain appears to be diagnostic for certain repetitive DNAs. The centromeric regions are densely heterochromatic, and further heterochromatic bands occur on the X-chromosome and on both arms of the B-chromosome. The significance of these results is discussed in rel......
DNA replication asynchrony between the paternal and maternal alleles of imprinted genes does not straddle the R/G transition
Chromosoma - Tập 106 - Trang 405-411 - 1997
Imprinted autosomal loci apparently reside in very large chromosomal domains that exhibit asynchrony in replication of homologous alleles during the DNA synthesis phase. Replication asynchrony can be cytogenetically visualized by a replication-banding discordance between homologous bands of a given pair of chromosomal homologs. The replication time of a chromosomal band at high resolution can be ......
Analysis of nucleolar transcription and processing domains and pre-rRNA movements by in situ hybridization
Chromosoma - Tập 105 - Trang 481-495 - 1997
We have examined the cytological localization of rRNA synthesis, transport, and processing events within the mammalian cell nucleolus by double-label fluorescent in situ hybridization analysis using probes for small selected segments of pre-rRNA, which have known half-lives. In particular, a probe for an extremely short-lived 5′ region that is not found separate of the pre-rRNA identifies nascent ......
Regulated specific proteolysis of the Cajal body marker protein coilin
Chromosoma - Tập 121 - Trang 629-642 - 2012
Cajal bodies (CB) are subnuclear domains that contain various proteins with diverse functions including the CB marker protein coilin. In this study, we investigate the proteolytic activity of calpain on coilin. Here, we report a 28-kDa cleaved coilin fragment detected by two coilin antibodies that is cell cycle regulated, with levels that are consistently reduced during mitosis. We further show th......
The effect of trifluoroperazine on microtubules, nuclear division and the nuclear membranes of the ciliate Nyctotherus ovalis
Chromosoma - Tập 93 - Trang 453-459 - 1986
Exposure of the dividing ciliate Nyctotherus ovalis to the tranquilizer trifluoroperazine (TFP; 10 μM) leads to the complete disassembly of kinetochore microtubules in the metaphase micronucleus. Interpolar microtubules located underneath the micronuclear envelope at anaphase and telophase stembody microtubules are more resistant to TFP. However, stembodies of drug-exposed ciliates are much shorte......
Preparation of chromosome spreads for electron (TEM, SEM, STEM), light and confocal microscopy
Chromosoma - Tập 103 - Trang 381-392 - 1994
In the past, ultrastructural studies on chromosome morphology have been carried out using light microscopy, scanning electron microscopy and transmission electron microscopy of whole mounted or sectioned samples. Until now, however, it has not been possible to use all of these techniques on the same specimen. In this paper we describe a specimen preparation method that allows one to study the same......
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