Localization of actin-related sequences by in situ hybridization to R-banded human chromosomesChromosoma - Tập 89 - Trang 163-167 - 1984
A. Bernheim, R. Berger, P. Szabo
Using a cytoplasmic actin cDNA probe we have localized a number of actin sequences in the human genome using a novel in situ hybridization technique. Metaphase chromosomes treated to produce R-bands were directly annealed with 125I-labeled actin probe. Under these conditions many regions of the genome were apparently denatured enough to be capable of hybridizing with the probe. Most of the actin s...... hiện toàn bộ
RegisterChromosoma - Tập 14 - Trang 676-692 - 1963
3H-Uridine labelling patterns in the Drosophila melanogaster salivary gland chromosomes X, 2R and 3LChromosoma - Tập 49 - Trang 219-231 - 1975
I. F. Zhimulev, E. S. Belyaeva
RNA-synthesizing regions in the polytene chromosomes of D. melanogaster were located; the level of 3H-uridine incorporation in each of these regions was determined. — It was found that the number of RNA-synthesizing regions is much larger than the number of puffs: almost all the chromosome regions are labelled except dense bands. In most cases, “non-puffed” regions label 10 to even 100 times weake...... hiện toàn bộ
The labeling efficiency of human telomeres is increased by double-strand PRINSChromosoma - Tập 113 - Trang 204-209 - 2004
Ju Yan, Bing-Zhen Chen, Eric F. Bouchard, Régen Drouin
Telomeres are composed of tandem repeated sequences, TTAGGG, that can be detected either by fluorescence in situ hybridization (FISH), more efficiently by using a peptide nucleic acid (PNA) probe, or by the primed in situ (PRINS) technique. However, the efficiency of human telomere labeling using PRINS is somewhat lower than the efficiency using PNA-FISH. To solve this problem, we developed a doub...... hiện toàn bộ
The process of a Y-loss event in an XO/XO mammal, the Ryukyu spiny ratChromosoma - Tập 119 - Trang 519-526 - 2010
Asato Kuroiwa, Yasuko Ishiguchi, Fumio Yamada, Abe Shintaro, Yoichi Matsuda
The Ryukyu spiny rat, Tokudaia osimensis, has an XO/XO sex chromosome constitution, lacking a Y chromosome and the mammalian sex-determining gene SRY. To investigate the Y-loss event, we traced three proto-Y-linked genes, RBMY1A1, EIF2S3Y, and KDM5D, in the genome. The original Y-linked RBMY1A1 was lost as well as SRY, and the remaining RBMY1A1 was a processed pseudogene on autosome. In contrast, ...... hiện toàn bộ