Canadian Science Publishing
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Studies on Spermatogenesis in Rats. I. Application of the Sedimentation Velocity Technique to an Investigation of Spermatogenesis
Canadian Science Publishing - Tập 49 Số 7 - Trang 753-760 - 1971
A sedimentation velocity chamber at unit gravity (Staput fractionation) was
employed to separate cells obtained from suspensions of testes from adult rats,
and the differential cell count was determined to estimate the sedimentation
profiles of cell populations in various fractions. The incorporation of
thymidine-3H into acid-insoluble portions of cell fractions was measured at
varying times follo...
Organization and dynamics of membrane lipids as determined by magnetic resonance spectroscopy
Canadian Science Publishing - Tập 57 Số 1 - Trang 1-14 - 1979
not available
THE COMPOSITIONS OF SOME PEPTIDES PRODUCED BY AN ENZYMIC HYDROLYSIS OF WHEAT GLIADIN
Canadian Science Publishing - Tập 42 Số 8 - Trang 1133-1140 - 1964
Wheat gliadin was hydrolyzed extensively by treatment with pepsin and then by
trypsin. A large number of peptides were produced as well as small amounts of
some amino acids, thus indicating a substantial amount of hydrolysis of gliadin
by the enzymes. Five peptides were obtained crystalline and two of these
constitute 11.5% and 1.2% of gliadin, respectively. Their structures have been
partly deter...
Effects of various conditions on the movement of carbon atoms derived from glucose into and out of protein in rat brain
Canadian Science Publishing - Tập 48 Số 3 - Trang 236-243 - 1970
The time course of entry of radioactive carbon from intravenously administered
[U-14C]-glucose into protein in five brain regions in rats was studied using an
automatic amino acid analyzer coupled, through a flow cell, with a scintillation
counter. Radioactivity appeared in protein-bound alanine and in glutamic and
aspartic acids and, more slowly and to a much lesser extent, in serine, proline,
an...
Comparison of the histones from fish erythrocytes
Canadian Science Publishing - Tập 55 Số 12 - Trang 1220-1227 - 1977
Because of contentions concerning the generality of cell-specific histone 5 (H5)
in nucleated erythrocytes of species other than birds, the erythrocyte histones
of carp, trout, perch, black crappie, and white sucker were studied under
conditions which minimize proteolysis. All were examined by polyacrylamide gel
electrophoresis, and histone 1 (H1) and H5 were purified by cation-exchange
chromatogr...
STEROID HORMONES IN THE PLASMA OF SPAWNED ATLANTIC SALMON (SALMO SALAR) AND A COMPARISON OF THEIR DETERMINATION BY CHEMICAL AND BIOLOGICAL ASSAY METHODS
Canadian Science Publishing - Tập 42 Số 2 - Trang 211-218 - 1964
11-Ketotestosterone, cortisone, and Cortisol were isolated and identified as the
principal steroid hormones in spawned male Atlantic salmon plasma and the
dihydroxyacetone steroids in spawned male and female Atlantic salmon plasma were
determined.A large plasma sample from spawned female Atlantic salmon was
analyzed for adrenocorticosteroids using both the mouse liver glycogen
deposition bioassay ...
MULTIPLE FORMS OF LACTATE DEHYDROGENASE AND ASPARTATE AMINOTRANSFERASE IN HERRING (CLUPEA HARENGUS HARENGUS L.)
Canadian Science Publishing - Tập 44 Số 10 - Trang 1319-1326 - 1966
The distribution of isoenzymes of lactate dehydrogenase (LDH) and aspartate
aminotransferase (AAT) in the tissues of 189 herring (Clupea harengus harengus
L.) were examined. Starch-gel electrophoresis of the LDH isoenzymes of the
herring revealed the presence of two hybrid forms representing mutant alleles at
the B locus. These mutants gave rise to two genotypes, BB′ and BB″, whose LDH
staining pa...
Glycerolipid metabolism in Ascaris lumbricoides
Canadian Science Publishing - Tập 46 Số 10 - Trang 1167-1173 - 1968
Homogenates of ovarian tissue from the pig roundworm Ascaris lumbricoides
rapidly incorporate fatty 14C-acids, fatty 14C-acyl-CoA derivatives, glucose,
and glycerol into lipid, the most highly labeled fraction after prolonged
incubation periods being the triglycerides (except with glucose). Data from
studies on the time course of incorporation of these precursors is consistent
with the operation o...
THE EFFECT OF LIPID MICELLES ON MITOCHONDRIAL MALATE DEHYDROGENASE
Canadian Science Publishing - Tập 45 Số 6 - Trang 839-851 - 1967
Mitochondrial malate dehydrogenase (M-MDH) is known to be very unstable in
aqueous solution. This instability may be ascribed to removal of the enzyme by
severe extraction procedures from its bound state in the mitochondrion.Whole
heart lipid micelles, mitochondrial lipids, and fatty acids increase the
stability of the enzyme. The degree of lipid ability to increase activity of
M-MDH is dependent ...
Steroid Metabolism by the Immature Rat Testis
Canadian Science Publishing - Tập 52 Số 9 - Trang 744-750 - 1974
Treatment of hypophysectomized immature male rats with luteinizing hormone (LH)
greatly increased the metabolism of both 4-[14C]progesterone and
4-[14C]testosterone by testicular homogenates. Prolactin, either alone or in
combination with LH, did not influence the metabolism of either substrate.
Progesterone was metabolized to 17α-hydroxyprogesterone, androstenedione,
5α-pregnan-3,20-dione, 3α-hyd...
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