Bulletin of Experimental Biology and Medicine

An objective method is presented for the purpose of evaluating the speed with which healing takes place in sutured wounds. The method of placing a wire loop and then removing it after 48 hours with the aid of a spring balance is explained. A short series of experiments was performed which attested to the general validity of the method.

We studied sex-related features of the microcirculation response of symmetrical areas of the human head to hypoxic loads. In 20 healthy volunteers (10 male and 10 female), short-term hypoxia was modeled using the ReOxy Cardio device (S. A. Aimediq). Microcirculation parameters were synchronously measured by laser Doppler flowmetry in the initial state and immediately after short-term hypoxic exposure. 3D graphs were constructed and regression equations were formulated that describe the relationships between changes in microcirculation on both sides of the measurement. Using a new method of geometric zoning, it was shown that the direction of reactions to hypoxia of the left microcirculatory bed is opposite in subjects of different sexes. In 80% male subjects, hypoxia led to an increase in perfusion on the left, in 20% — to a decrease. In female subjects, the picture was opposite: perfusion decreased in 90% and increased in 10% subjects. It can be assumed that these differences in the reactions of microcirculation to hypoxia in men and women are an additional confirmation of different structural and functional organization of the male and female brain, which leads to different resistance of males and females to external disturbing influences. The results of the study can be used in medical practice to develop methods for personalized assessments of circulatory disorders and differentiated approaches to the treatment of men and women with cardiovascular diseases.

We studied changes in the blood cytokine profile of rats 3 h, 1 day, and 8 days after acute stress on the model of 24-h immobilization followed by LPS administration (100 μg/kg intraperitoneally). The concentration of proinflammatory cytokines (particularly of IL-1β and TNFα) significantly decreased at the early stage after stress exposure and physiological saline injection, but increased in the follow-up period and practically did not differ or even surpassed the control level by the end of observations. Under these conditions, the blood content of anti-inflammatory cytokine IL-10 increased most significantly on day 1 of the post-stress period. Restraint stress followed by LPS administration was accompanied by a decrease in the level of proinflammatory cytokines at the early (IFNγ and TNFα) and late stages (IL-1β) of the experiment. Directed modulation of the immune status in animals after acute stress was followed by a significant increase in the content of IL-10 on days 1 and 8, as well as by a tendency toward elevation of IL-4 concentration by the end of the study. The directionality and degree of changes in the cytokine profile of mammalian tissues depend on the type of extreme exposure, duration of the post-stress period, and specific effects of exogenous pathogenic factors in the whole body.

Normalization of the menstrual function in women with spontaneous abortion receiving sorbent and bioresonance (extremely high-frequency) therapy was evaluated by morphological criteria (correspondence of structural changes in the endometrium to the phase of the menstrual cycle). The absence of microvilli and cilia on the apical surface of surface and glandular epitheliocytes, presence of intranuclear tubules and giant mitochondria in the cytoplasm, and signs of apocrine secretion in epitheliocytes attested to the luteal phase of the cycle.

The levels of IL-8 and IL-10 and their ratio depending on the stage and NYHA functional class of heart failure were determined in the patients with chronic heart failure in comparison with healthy individuals. The level of IL-8 and IL-8/IL-10 ratio in patients were significantly higher than in controls, and the level of IL-10, significantly lower. The comparison of cytokine levels in patients at different stages of heart failure and NYHA class revealed no significant differences. However, the level of IL-10 tended to decrease by stage 3 of the disease. Significantly elevated IL-8 level along with reduced IL-10 level and significantly increased IL-8/IL-10 ratio indicated predominance of proinflammatory activity in patients with chronic heart failure, which along with the tendency to decrease IL-10 level at stage 3 of the disease may indicate the ineffectiveness of compensatory mechanisms and progressive “failure” of compensation with increasing severity of the disease.