The acrosome reaction in human spermatozoaBiology of the Cell - Tập 92 - Trang 255-266 - 2000
Catherine Patrat, Catherine Serres, Pierre Jouannet
During gamete interaction, sperm acrosome reaction (AR) induced by oocyte investment is a prerequisite event for the spermatozoa to pass through the zona pellucida (ZP), fuse with and penetrate the oocyte. Progesterone (P4), secreted by cumulus cells, is an important cofactor for the occurrence of this exocytosis event. The AR results from the fusion between outer acro...... hiện toàn bộ
A simple technique for the long‐term non‐polarised and polarised culture of human fallopian tube epithelial cellsBiology of the Cell - Tập 82 - Trang 103-107 - 1994
M.E. Kervancioglu, E. Saridogan, J.E. Martin, S.D. Maguiness, O. Djahanbakhch
Summary— Fallopian tubes were obtained from 25 women undergoing abdominal hysterectomy. Pieces of fallopian tube mucosa were placed in culture flasks containing minimum essential medium in Earle's salts supplemented with fetal bovine serum. First passage was carried out after 7–10 days and subcultures in 4–5 days. For polarised cell culture, epithelial cells were see...... hiện toàn bộ
Regulation of the P25 gene transcription in the silk gland of BombyxBiology of the Cell - Tập 84 - Trang 43-52 - 1995
Pascale Nony, Jean-Claude Prudhomme, Pierre Couble
Summry— The gene encoding the silk protein P25 is specifically transcribed in the posterior silkgland of Bombyx during larval intermoults and is repressed during moults. By performing in vitro DNA‐protein interactions, at least five putative regulatory elements were localized in the 5′ flanking region of the gene....... hiện toàn bộ