Biochemical Journal
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bFGF and aFGF induce membrane ruffling in breast cancer cells but not in normal breast epithelial cells: FGFR-4 involvementAcidic and basic fibroblast growth factors (aFGF and bFGF) are growth factors which may have a physiological role in the normal breast and in breast cancer. A study of the effects of aFGF and bFGF on a variety of breast cell lines and epithelial cells purified from normal breast organoids showed that whereas normal breast cells did not exhibit membrane ruffling in response to either of the... ... hiện toàn bộ
Biochemical Journal - Tập 306 Số 2 - Trang 609-616 - 1995
Involvement of δ-aminolaevulinate synthase encoded by the parasite gene in de novo haem synthesis by Plasmodium falciparumThe malaria parasite can synthesize haem de novo. In the present study, the expression of the parasite gene for Δ-aminolaevulinate synthase (PfALAS) has been studied by reverse transcriptase PCR analysis of the mRNA, protein expression using antibodies to the recombinant protein expressed in Escherichia coli and assay of ALAS enzyme activity in Plasmodium falciparum in culture. The gene is... ... hiện toàn bộ
Biochemical Journal - Tập 367 Số 2 - Trang 321-327 - 2002
The mechanism of initiation of lipid peroxidation. Evidence against a requirement for an iron(II)-iron(III) complexWhen Fe2+ ions are added to rat-liver microsomes, lipid peroxidation begins after a short lag period. Fe2+-dependent peroxidation in the first few minutes of the incubation can be increased by adding Fe3+, ascorbic acid or Pb2+ ions; these stimulations are not additive. By contrast, Pb2+ ions inhibit peroxidation of microsomes in the presence of Fe3+/ascorbate or Fe3+-ADP/NADPH. In liposom... ... hiện toàn bộ
Biochemical Journal - Tập 258 Số 2 - Trang 617-620 - 1989
MAC inhibitors suppress mitochondrial apoptosisMAC (mitochondrial apoptosis-induced channel) forms in the mitochondrial outer membrane and unleashes cytochrome c to orchestrate the execution of the cell. MAC opening is the commitment step of intrinsic apoptosis. Hence closure of MAC may prevent apoptosis. Compounds that blocked the release of fluorescein from liposomes by recombinant Bax were tested for their ability to directly close ... ... hiện toàn bộ
Biochemical Journal - Tập 423 Số 3 - Trang 381-387 - 2009
Mitochondrial priming modifies Ca2+ oscillations and insulin secretion in pancreatic isletsIncreases in mitochondrial [Ca2+] ([Ca2+]m) have recently been reported to cause long-term alterations in cellular ATP production [Jouaville, Bastianutto, Rutter and Rizzuto (1999) Proc. Natl. Acad. Sci. U.S.A. 96, 13807Ő13812]. We have determined the importance of this phenomenon for nutrient sensing in pancreatic islets and β-cells by imaging adenovirally expressed Ca2+ and ATP sensors (... ... hiện toàn bộ
Biochemical Journal - Tập 353 Số 2 - Trang 175-180 - 2001
The metabolism in vitro of human low-density lipoprotein by the human hepatoma cell line Hep G2The human hepatoma cell line Hep G2 was studied with respect to metabolism of human low-density lipoprotein (LDL). The Hep G2 cells bind, take up and degrade human LDL with a high-affinity saturable and with a low-affinity non-saturable component. The high-affinity binding possesses a KD of 25 nM-LDL and a maximal amount of binding of about 70 ng of LDL-apoprotein/mg of cell protein. The h... ... hiện toàn bộ
Biochemical Journal - Tập 214 Số 3 - Trang 951-958 - 1983
Structural and functional dissection of a conserved destabilizing element of cyclo-oxygenase-2 mRNA: evidence against the involvement of AUF-1 [AU-rich element/poly(U)-binding/degradation factor-1], AUF-2, tristetraprolin, HuR (Hu antigen R) or FBP1 (far-upstream-sequence-element-binding protein 1)COX-2 (cyclo-oxygenase-2) mRNA is degraded rapidly in resting cells, but is stabilized by the mitogen-activated protein kinase p38 signalling pathway in response to pro-inflammatory stimuli. A conserved ARE (AU-rich element) of the COX-2 3´ untranslated region, CR1 (conserved region 1), acts as a potent instability determinant, and mediates stabilization in response to p38 activation. A de... ... hiện toàn bộ
Biochemical Journal - Tập 377 Số 3 - Trang 629-639 - 2004
Identification of Drosophila melanogaster yellow-f and yellow-f2 proteins as dopachrome-conversion enzymesThis study describes the identification of Drosophila yellow-f and yellow-f2 as dopachrome-conversion enzymes responsible for catalysing the conversion of dopachrome into 5,6-dihydroxyindole in the melanization pathway. Drosophilayellow-y gene and yellow-b, -c, -f and -f2 genes were expressed in an insect cell/baculovirus expression system and their corresponding recombinant proteins were ... ... hiện toàn bộ
Biochemical Journal - Tập 368 Số 1 - Trang 333-340 - 2002
Properties of follicle-stimulating-hormone receptor in cell membranes of bovine testisA simple method for preparing plasma membranes from bovine testes is described. Bovine testicular receptor has a high affinity and specificity for 125I-labelled human FSH (follicle-stimulating hormone). The specific binding of 125I-labelled human FSH to the plasma membranes is a saturable process with respect to the amounts of receptor protein and FSH added. The association and dissociatio... ... hiện toàn bộ
Biochemical Journal - Tập 149 Số 1 - Trang 123-132 - 1975
Functions of NF-κB1 and NF-κB2 in immune cell biologyTwo members of the NF-κB (nuclear factor κB)/Rel transcription factor family, NF-κB1 and NF-κB2, are produced as precursor proteins, NF-κB1 p105 and NF-κB2 p100 respectively. These are proteolytically processed by the proteasome to produce the mature transcription factors NF-κB1 p50 and NF-κB2 p52. p105 and p100 are known to function additionally as IκBs (inhibitors of NF-κB), which retain... ... hiện toàn bộ
Biochemical Journal - Tập 382 Số 2 - Trang 393-409 - 2004
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