Cloning of a lymphatic peptide/histidine transporterBiochemical Journal - Tập 356 Số 1 - Trang 53-60 - 2001
Kazuko Sakata, Toshihide Yamashita, Mitsuyo MAEDA, Yoshinori Moriyama, Shoichi Shimada, Masaya TOHYAMA
Although peptide transport across the plasma membrane has been characterized well in the kidney and the intestine, the functional relevance of this transport in other organs has not been addressed. Here we report the cloning of a cDNA for a novel peptide/histidine transporter found in the rat (rPHT2), whose mRNA is expressed mainly in the lymphatic system. rPHT2 encodes a protein of 582 am...... hiện toàn bộ
Lyso-glycosphingolipids mobilize calcium from brain microsomes via multiple mechanismsBiochemical Journal - Tập 375 Số 3 - Trang 561-565 - 2003
Emyr Lloyd‐Evans, Dori Pelled, Christian Riebeling, Anthony H. Futerman
Recently, we demonstrated that the GSL (glycosphingolipid), GlcCer (glucosylceramide), modulates Ca2+ release from intracellular stores and from microsomes by sensitizing the RyaR (ryanodine receptor), a major Ca2+-release channel of the endoplasmic reticulum, whereas the lyso derivative of GlcCer, namely GlcSph (glucosylsphingosine), induced Ca2+ release via a mechanism independent of the...... hiện toàn bộ
Pathways of peroxynitrite oxidation of thiol groupsBiochemical Journal - Tập 322 Số 1 - Trang 167-173 - 1997
Celia Quijano, Beatriz Álvarez, R Gatti, Ohára Augusto, Rafael Radí
Peroxynitrite mediates the oxidation of the thiol group of both cysteine and glutathione. This process is associated with oxygen consumption. At acidic pH and a cysteine/peroxynitrite molar ratio of ≤ 1.2, there was a single fast phase of oxygen consumption, which increased with increasing concentrations of both cysteine and oxygen. At higher molar ratios the profile of oxygen consumption ...... hiện toàn bộ
Monoclonal antibodies reacting with multiple epitopes on the human insulin receptorBiochemical Journal - Tập 235 Số 1 - Trang 199-208 - 1986
Maria A. Soos, Kenneth Siddle, Michael D. Baron, JM Heward, J. Paul Luzio, J. Bellatin, Edwin S. Lennox
Monoclonal antibodies for the human insulin receptor were produced following immunization of mice with IM-9 lymphocytes and/or purified placental receptor. Four separate fusions yielded 28 antibodies, all of which reacted with receptor from human placenta, liver and IM-9 cells. Some antibodies cross-reacted to varying degrees with receptor from rabbit, cow, pig and sheep, but none reacted ...... hiện toàn bộ
Transaldolase deficiency influences the pentose phosphate pathway, mitochondrial homoeostasis and apoptosis signal processingBiochemical Journal - Tập 415 Số 1 - Trang 123-134 - 2008
Yueming Qian, Sanjoy Banerjee, Craig E. Grossman, Wendy Amidon, György Nagy, Maureen Barcza, Brian Niland, David R. Karp, Frank A. Middleton, Katalin Bánki, András Perl
TAL (transaldolase) was originally described in the yeast as an enzyme of the PPP (pentose phosphate pathway). However, certain organisms and mammalian tissues lack TAL, and the overall reason for its existence is unclear. Recently, deletion of Ser171 (TALΔS171) was found in five patients causing inactivation, proteasome-mediated degradation and complete deficiency of TAL. In the present s...... hiện toàn bộ
Near-infrared magnetic and natural circular dichroism of cytochrome c oxidaseBiochemical Journal - Tập 191 Số 2 - Trang 319-331 - 1980
D G Eglinton, Michael K. Johnson, Andrew J. Thomson, P E Gooding, C Greenwood
A detailed study is presented of the room-temperature absorption, natural and magnetic circulation-dichroism (c.d. and m.c.d.) spectra of cytochrome c oxidase and a number of its derivatives in the wavelength range 700-1900 nm. The spectra of the reduced enzyme show a strong negative c.d. band peaking at 1100nm arising from low-spin ferrous haem a and a positive m.c.d. peak at 780nm assign...... hiện toàn bộ
Na+/H+ exchange modulates the production of leukotriene B4 by human neutrophilsBiochemical Journal - Tập 257 Số 3 - Trang 751-758 - 1989
Mitsuhiko Osaki, Hidetoshi Sumimoto, Koichiro Takeshige, Edward J. Cragoe, Yuta Hori, Shigeki Minakami
Human neutrophils produce various compounds of the 5-lipoxygenase pathway, including (5S)-hydroxyeicosatetraenoic acid, leukotriene B4, its 6-trans isomers and omega-oxidation metabolites of LTB4, when the cells are stimulated with the Ca2+ ionophore A23187. The elevation in the extracellular pH (pHo) facilitated the cytoplasmic alkalinization induced by the ionophore as determined fluorom...... hiện toàn bộ
Endoplasmic reticulum chaperones inhibit the production of amyloid-β peptidesBiochemical Journal - Tập 402 Số 3 - Trang 581-589 - 2007
Tatsuya Hoshino, Tadashi Nakaya, Wataru Araki, Keitarou Suzuki, Toshiharu Suzuki, Tohru Mizushima
Aβ (amyloid-β peptides) generated by proteolysis of APP (β-amyloid precursor protein), play an important role in the pathogenesis of AD (Alzheimer's disease). ER (endoplasmic reticulum) chaperones, such as GRP78 (glucose-regulated protein 78), make a major contribution to protein quality control in the ER. In the present study, we examined the effect of overexpression of various ER chapero...... hiện toàn bộ