SYBR Green real-time reverse transcription-polymerase chain reaction assay for the generic detection of coronavirusesArchives of Virology - Tập 152 - Trang 41-58 - 2006
S. Escutenaire, N. Mohamed, M. Isaksson, P. Thorén, B. Klingeborn, S. Belák, M. Berg, J. Blomberg
Coronaviruses are etiologic agents of respiratory and enteric diseases in humans and in animals. In this study, a one-step real-time reverse transcription-polymerase chain reaction (RT-PCR) assay based on SYBR Green chemistry and degenerate primers was developed for the generic detection of coronaviruses. The primers, designed in the open reading frame 1b, enabled the detection of 32 animal corona...... hiện toàn bộ
Complete genome sequence of a novel mitovirus isolated from the fungus Fusarium equiseti causing potato dry rotArchives of Virology - Tập 167 Số 12 - Trang 2777-2781 - 2022
Zhang, Xiaofang, Li, Siwei, Ma, Zhihao, Cai, Qingnian, Zhou, Tao, Wu, Xuehong
In this study, a novel mitovirus was isolated from the fungus Fusarium equiseti causing potato dry rot and tentatively designated as "Fusarium equiseti mitovirus 1" (FeMV1). The full-length genome sequence of FeMV1 consists of 2,459 nucleotides with a predicted A + U content of 69.5%. Using the mold mitochondrial genetic code, an open reading frame (ORF) of 725 amino acids (aa) was predicted to en...... hiện toàn bộ
In vitro secondary 19 S and 7 S antibody responses to poliovirus in membrane cultures of separated spleen cellsArchives of Virology - Tập 15 - Trang 261-274 - 1965
Sven -Eric Svehag
Membrane cultures of separated spleen cells, from rabbits injected once with, poliovirus, formed antibodyin vitro for 2–3 weeks. Restimulation of spleen cells in suspension with homologous antigen evoked secondary antibody responses. The secondary 19 S antibody response was rapid and transient. The secondary 7 S response, which appeared to be biphasic, was of longer duration and reached peak titer...... hiện toàn bộ
Efficient rescue of infectious bursal disease virus using a simplified RNA polymerase II-based reverse genetics strategyArchives of Virology - Tập 153 - Trang 1131-1137 - 2008
N. Ben Abdeljelil, N. Khabouchi, H. Mardassi
We describe a simplified RNA polymerase II-based reverse genetics approach that allows for the efficient rescue of high-titer infectious bursal disease virus (IBDV) from cloned cDNAs of genomic segments A and B. Unlike the previously reported RNA polymerase II-based methods, the developed strategy does not necessitate the introduction of a ribozyme sequence at both ends of the genomic cDNA sequenc...... hiện toàn bộ
Comparative immunofluorescent studies on measles, canine distemper, and rinderpest virusesArchives of Virology - Tập 29 - Trang 90-100 - 1970
K. Yamanouchi, F. Kobune, A. Fukuda, M. Hayami, A. Shishido
The development of specific antigens of measles, canine distemper and rinderpest viruses in Vero cells was examined by fluorescent antibody (FA) techniques. Intracellular and extracellular virus antigens were demonstrated by FA staining of fixed cells and unfixed living cells, respectively. All the three viruses showed the same pattern of development of specific antigens in the same host cells. Bo...... hiện toàn bộ
Insect-specific flaviviruses in Aedes mosquitoes in GreeceArchives of Virology - Tập 161 - Trang 2183-2188 - 2016
Anna Papa, Elpida Papadopoulou, Ravish Paliwal, Stella Kalaitzopoulou, Spiros Mourelatos, Matthias Niedrig
Mosquitoes of the genus Aedes are known vectors of pathogenic flaviviruses, and insect-specific flaviviruses (ISFs) have been detected in members of this genus in numerous parts of the world. In order to gain insight into whether Aedes mosquitoes in Greece are infected by flaviviruses, 1173 Aedes spp. mosquitoes collected in 2010 and 2012 were grouped in 53 pools and tested by RT nested PCR using ...... hiện toàn bộ
Distribution of infectious endogenous retroviruses in mixed-breed and purebred catsArchives of Virology - Tập 165 Số 1 - Trang 157-167 - 2020
Minh Ha Ngo, Takehisa Soma, Hwa‐Young Youn, Taiji Endo, Isaac Makundi, Jun Kawasaki, Ariko Miyake, Bui Thi To Nga, Huyen Nguyen, María Cruz Arnal, Daniel Fernández de Luco, R. M. C. Deshapriya, Shingo Hatoya, Kazuo Nishigaki