SYBR Green real-time reverse transcription-polymerase chain reaction assay for the generic detection of coronavirusesArchives of Virology - Tập 152 - Trang 41-58 - 2006
S. Escutenaire, N. Mohamed, M. Isaksson, P. Thorén, B. Klingeborn, S. Belák, M. Berg, J. Blomberg
Coronaviruses are etiologic agents of respiratory and enteric diseases in humans
and in animals. In this study, a one-step real-time reverse
transcription-polymerase chain reaction (RT-PCR) assay based on SYBR Green
chemistry and degenerate primers was developed for the generic detection of
coronaviruses. The primers, designed in the open reading frame 1b, enabled the
detection of 32 animal corona... hiện toàn bộ
Complete genome sequence of a novel potyvirus isolated from Polygonatum kingianumArchives of Virology - Tập 165 - Trang 2127-2131 - 2020
Zeli Chen, Rex Frimpong Anane, Zhe Wang, Linyi Yang, Lu Chen, Guosong Wen, Mingfu Zhao
The complete genome sequence of a putative novel potyvirus, tentatively named
“Polygonatum kingianum virus 1” (PKgV1), infecting Polygonatum kingianum in
China was determined (GenBank accession no. MK427056). PKgV1 has a genome
organization that is typical of potyviruses, with a single large open reading
frame (nt 123–9236) that encodes a 3037-aa polyprotein that is predicted to be
cleaved into 10... hiện toàn bộ
Absence of interaction of genomic components and complementation between Mungbean yellow mosaic India virus isolates in cowpeaArchives of Virology - Tập 150 - Trang 1833-1844 - 2005
B. Surendranath, K. S. Usharani, A. Nagma, A. K. Victoria, V. G. Malathi
Agroinoculations were performed with DNA A and DNA B components of Mungbean
yellow mosaic India virus (MYMIV) isolates differing in their infectivity on
cowpea. Exchange of genomic components of the MYMIV isolates occurred in all the
leguminous species but not in cowpea. Extremely low viral DNA accumulation and
atypical leaf curl symptoms produced by reassortants in cowpea suggest barriers
both fo... hiện toàn bộ
Complete genome sequence of a novel mitovirus isolated from the fungus Fusarium equiseti causing potato dry rotArchives of Virology - Tập 167 Số 12 - Trang 2777-2781 - 2022
Zhang, Xiaofang, Li, Siwei, Ma, Zhihao, Cai, Qingnian, Zhou, Tao, Wu, Xuehong
In this study, a novel mitovirus was isolated from the fungus Fusarium equiseti
causing potato dry rot and tentatively designated as "Fusarium equiseti
mitovirus 1" (FeMV1). The full-length genome sequence of FeMV1 consists of 2,459
nucleotides with a predicted A + U content of 69.5%. Using the mold
mitochondrial genetic code, an open reading frame (ORF) of 725 amino acids (aa)
was predicted to en... hiện toàn bộ
Occurrence of Hsw1N1 subtype influenza A viruses in wild ducks in EuropeArchives of Virology - Tập 63 - Trang 185-190 - 1980
K. Ottis, P. A. Bachmann
Two identical strains of influenza A viruses antigenically related to swine
influenza (Hsw1N1) have been isolated from adult mallard ducks(Anas
platyrhynchos) in Southern Germany. They were designated A/Duck/Bavaria/1/77 and
A/Duck/Bavaria/2/77. Serologic tests revealed a close antigenic relationship to
the strain A/Duck/Alberta/35/76. Experimental infections of piglets with strain
A/Duck/Bavaria/... hiện toàn bộ
In vitro secondary 19 S and 7 S antibody responses to poliovirus in membrane cultures of separated spleen cellsArchives of Virology - Tập 15 - Trang 261-274 - 1965
Sven -Eric Svehag
Membrane cultures of separated spleen cells, from rabbits injected once with,
poliovirus, formed antibodyin vitro for 2–3 weeks. Restimulation of spleen cells
in suspension with homologous antigen evoked secondary antibody responses. The
secondary 19 S antibody response was rapid and transient. The secondary 7 S
response, which appeared to be biphasic, was of longer duration and reached peak
titer... hiện toàn bộ
Efficient rescue of infectious bursal disease virus using a simplified RNA polymerase II-based reverse genetics strategyArchives of Virology - Tập 153 - Trang 1131-1137 - 2008
N. Ben Abdeljelil, N. Khabouchi, H. Mardassi
We describe a simplified RNA polymerase II-based reverse genetics approach that
allows for the efficient rescue of high-titer infectious bursal disease virus
(IBDV) from cloned cDNAs of genomic segments A and B. Unlike the previously
reported RNA polymerase II-based methods, the developed strategy does not
necessitate the introduction of a ribozyme sequence at both ends of the genomic
cDNA sequenc... hiện toàn bộ
Complete genome sequences of two isolates of spiraea yellow leafspot virus (genus Badnavirus) from Spiraea x bumalda ‘Anthony Waterer’Archives of Virology - Tập 167 - Trang 631-634 - 2022
R. A. Alvarez-Quinto, Joana Serrano, Neil Olszewski, Samuel Grinstead, Dimitre Mollov, B. E. L. Lockhart
The complete genome sequences of two isolates of spiraea yellow leafspot virus
(SYLSV) were determined. Spiraea (Spiraea x bumalda) ‘Anthony Waterer’ plants
showing virus-like symptoms including yellow spotting and leaf deformation were
used for sequencing. The viral genome of SYLSV-MN (Minnesota) and SYLSV-MD
(Maryland) is 8,017bp in length. The sequences share 95% identity at the
nucleotide leve... hiện toàn bộ