SYBR Green real-time reverse transcription-polymerase chain reaction assay for the generic detection of coronavirusesArchives of Virology - Tập 152 - Trang 41-58 - 2006
S. Escutenaire, N. Mohamed, M. Isaksson, P. Thorén, B. Klingeborn, S. Belák, M. Berg, J. Blomberg
Coronaviruses are etiologic agents of respiratory and enteric diseases in humans and in animals. In this study, a one-step real-time reverse transcription-polymerase chain reaction (RT-PCR) assay based on SYBR Green chemistry and degenerate primers was developed for the generic detection of coronaviruses. The primers, designed in the open reading frame 1b, enabled the detection of 32 animal corona...... hiện toàn bộ
Complete genome sequence of a novel potyvirus isolated from Polygonatum kingianumArchives of Virology - Tập 165 - Trang 2127-2131 - 2020
Zeli Chen, Rex Frimpong Anane, Zhe Wang, Linyi Yang, Lu Chen, Guosong Wen, Mingfu Zhao
The complete genome sequence of a putative novel potyvirus, tentatively named “Polygonatum kingianum virus 1” (PKgV1), infecting Polygonatum kingianum in China was determined (GenBank accession no. MK427056). PKgV1 has a genome organization that is typical of potyviruses, with a single large open reading frame (nt 123–9236) that encodes a 3037-aa polyprotein that is predicted to be cleaved into 10...... hiện toàn bộ
Absence of interaction of genomic components and complementation between Mungbean yellow mosaic India virus isolates in cowpeaArchives of Virology - Tập 150 - Trang 1833-1844 - 2005
B. Surendranath, K. S. Usharani, A. Nagma, A. K. Victoria, V. G. Malathi
Agroinoculations were performed with DNA A and DNA B components of Mungbean yellow mosaic India virus (MYMIV) isolates differing in their infectivity on cowpea. Exchange of genomic components of the MYMIV isolates occurred in all the leguminous species but not in cowpea. Extremely low viral DNA accumulation and atypical leaf curl symptoms produced by reassortants in cowpea suggest barriers both fo...... hiện toàn bộ
Complete genome sequence of a novel mitovirus isolated from the fungus Fusarium equiseti causing potato dry rotArchives of Virology - Tập 167 Số 12 - Trang 2777-2781 - 2022
Zhang, Xiaofang, Li, Siwei, Ma, Zhihao, Cai, Qingnian, Zhou, Tao, Wu, Xuehong
In this study, a novel mitovirus was isolated from the fungus Fusarium equiseti causing potato dry rot and tentatively designated as "Fusarium equiseti mitovirus 1" (FeMV1). The full-length genome sequence of FeMV1 consists of 2,459 nucleotides with a predicted A + U content of 69.5%. Using the mold mitochondrial genetic code, an open reading frame (ORF) of 725 amino acids (aa) was predicted to en...... hiện toàn bộ
Occurrence of Hsw1N1 subtype influenza A viruses in wild ducks in EuropeArchives of Virology - Tập 63 - Trang 185-190 - 1980
K. Ottis, P. A. Bachmann
Two identical strains of influenza A viruses antigenically related to swine influenza (Hsw1N1) have been isolated from adult mallard ducks(Anas platyrhynchos) in Southern Germany. They were designated A/Duck/Bavaria/1/77 and A/Duck/Bavaria/2/77. Serologic tests revealed a close antigenic relationship to the strain A/Duck/Alberta/35/76. Experimental infections of piglets with strain A/Duck/Bavaria/...... hiện toàn bộ
In vitro secondary 19 S and 7 S antibody responses to poliovirus in membrane cultures of separated spleen cellsArchives of Virology - Tập 15 - Trang 261-274 - 1965
Sven -Eric Svehag
Membrane cultures of separated spleen cells, from rabbits injected once with, poliovirus, formed antibodyin vitro for 2–3 weeks. Restimulation of spleen cells in suspension with homologous antigen evoked secondary antibody responses. The secondary 19 S antibody response was rapid and transient. The secondary 7 S response, which appeared to be biphasic, was of longer duration and reached peak titer...... hiện toàn bộ
Efficient rescue of infectious bursal disease virus using a simplified RNA polymerase II-based reverse genetics strategyArchives of Virology - Tập 153 - Trang 1131-1137 - 2008
N. Ben Abdeljelil, N. Khabouchi, H. Mardassi
We describe a simplified RNA polymerase II-based reverse genetics approach that allows for the efficient rescue of high-titer infectious bursal disease virus (IBDV) from cloned cDNAs of genomic segments A and B. Unlike the previously reported RNA polymerase II-based methods, the developed strategy does not necessitate the introduction of a ribozyme sequence at both ends of the genomic cDNA sequenc...... hiện toàn bộ
Detection of herpesviral sequences in tissues of green turtles with fibropapilloma by polymerase chain reactionArchives of Virology - Tập 145 - Trang 1885-1893 - 2014
Y. Lu, Y. Wang, Q. Yu, A. A. Aguirre, G. H. Balazs, V. R. Nerurkar, R. Yanagihara
An alpha-herpesvirus has been associated recently with green turtle fibropapilloma (FP). To further clarify the role of this newfound green turtle herpesvirus (GTHV) in the pathogenesis of FP, various normal-appearing tissues and organs (including skin, eye, brain, heart, liver, spleen, intestine, lung, kidney, nerve, gonad, tongue, gall bladder, urinary bladder, thyroid and peripheral blood mono...... hiện toàn bộ