American Society for Microbiology

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Acute Inflammatory Response to Endotoxin in Mice and Humans
American Society for Microbiology - Tập 12 Số 1 - Trang 60-67 - 2005
Shannon Copeland, H. Shaw Warren, Stephen F. Lowry, Steve E. Calvano, Daniel G. Remick
ABSTRACTEndotoxin injection has been widely used to study the acute inflammatory response. In this study, we directly compared the inflammatory responses to endotoxin in mice and humans.Escherichia colitype O113 endotoxin was prepared under identical conditions, verified to be of equal biological potency, and used for both mice and humans...... hiện toàn bộ
Human Immunodeficiency Virus Persistence and Production in T-Cell Development
American Society for Microbiology - Tập 13 Số 11 - Trang 1237-1245 - 2006
Kevin B. Gurney, Christel H. Uíttenbogaart
ABSTRACTHuman immunodeficiency virus type 1 (HIV-1) replication depends on CD4 and coreceptor expression as well as host factors associated with the activation state of the cell. To determine the impact of the activation stage of thymocytes on the HIV-1 life cycle, we investigated R5 and X4 HIV-1 entry, reverse transcription, and expression in discrete thymocyte su...... hiện toàn bộ
Septic Shock after Seasonal Influenza Vaccination in an HIV-Infected Patient during Treatment with Etanercept for Rheumatoid Arthritis: a Case Report
American Society for Microbiology - Tập 20 Số 5 - Trang 761-764 - 2013
Pasquale De Nardo, Rita Bellagamba, Angela Corpolongo, Elisa Gentilotti, Fabrizio Taglietti, Silvia Rosati, Mauro Galeazzi, Gian Domenico Sebastiani, Isabella Quinti, Emanuele Nicastri
ABSTRACTAnti-tumor necrosis factor alpha (anti-TNF-α) is used in the treatment of rheumatic diseases not responsive to first-line regimens. Data on the safety of anti-TNF-α in HIV-infected patients are scarce and conflicting. We describe a case of septic shock and multiorgan failure that occurred after etanercept initiation and influenza vaccination in an HIV-infec...... hiện toàn bộ
Clearance of Virulent but Not AvirulentRhodococcus equifrom the Lungs of Adult Horses Is Associated with Intracytoplasmic Gamma Interferon Production by CD4+and CD8+T Lymphocytes
American Society for Microbiology - Tập 10 Số 2 - Trang 208-215 - 2003
Stephen A. Hines, Diana M. Stone, Melissa T. Hines, Debby C. Alperin, Donald P. Knowles, Linda K. Norton, Mary Jo Hamilton, William C. Davis, Travis C. McGuire
ABSTRACTRhodococcus equiis a gram-positive bacterium that infects alveolar macrophages and causes rhodococcal pneumonia in horses and humans. The virulence plasmid ofR. equiappears to be required for both pathogenicity in the horse and the induction of protective immunity. An understanding of the mechanisms by w...... hiện toàn bộ
Optimization and Validation of a Multiplex, Electrochemiluminescence-Based Detection Assay for the Quantitation of Immunoglobulin G Serotype-Specific Antipneumococcal Antibodies in Human Serum
American Society for Microbiology - Tập 16 Số 3 - Trang 387-396 - 2009
Rocio D. Marchese, Derek Puchalski, Pamela Miller, Joseph Antonello, Olivia Hammond, Tina Green, Leonard J. Rubinstein, Michael J. Caulfield, Daniel J. Sikkema
ABSTRACT Pneumovax 23 consists of a mixture of highly purified capsular polysaccharides (Ps) from 23 of the most prevalent serotypes of Streptococcus pneumoniae . Testing of vaccine immunogenicity has been historically performed on the enzyme-linked immunosorbent assay (ELISA) platform, valid...... hiện toàn bộ
Use of Opsonophagocytosis for Serological Evaluation of Pneumococcal Vaccines
American Society for Microbiology - Tập 13 Số 2 - Trang 165-169 - 2006
Sandra Romero‐Steiner, Carl E. Frasch, George M. Carlone, Roland A. Fleck, David Goldblatt, Moon H. Nahm
Development and Validation of a Fourfold Multiplexed Opsonization Assay (MOPA4) for Pneumococcal Antibodies
American Society for Microbiology - Tập 13 Số 9 - Trang 1004-1009 - 2006
Robert L. Burton, Moon H. Nahm
ABSTRACT Opsonophagocytic killing assays (OPAs) are essential for developing and improving pneumococcal vaccines. There is a need for a high-throughput, reliable, standardized, and fully characterized OPA for pneumococcal antibodies. To meet the need, we have developed and characterized a fourfold multiplexed OPA (MOPA4) against 13 serotypes ...... hiện toàn bộ
Differentiation, Maturation, and Survival of Dendritic Cells by Osteopontin Regulation
American Society for Microbiology - Tập 12 Số 1 - Trang 206-212 - 2005
Kodai Kawamura, Kazuhiro Iyonaga, Hidenori Ichiyasu, Junko Nagano, Moritaka Suga, Yutaka Sasaki
ABSTRACTDendritic cells (DCs) are antigen-presenting cells with the ability to induce primary immune responses necessary in innate immunity and adaptive immunity. Osteopontin (OPN) is a secreted acidic phosphoprotein containing an arginine-glycine-aspartate sequence and has been suggested to play an important role in early cellular immune responses. The interaction...... hiện toàn bộ
Microneedle Delivery of H5N1 Influenza Virus-Like Particles to the Skin Induces Long-Lasting B- and T-Cell Responses in Mice
American Society for Microbiology - Tập 17 Số 9 - Trang 1381-1389 - 2010
Jae-Min Song, Yeu‐Chun Kim, Aleksandr S. Lipatov, Marc Pearton, C. Todd Davis, Dae‐Goon Yoo, Kyoung‐mi Park, Li-Mei Chen, Fu‐Shi Quan, James C. Birchall, Ruben O. Donis, Mark R. Prausnitz, Richard W. Compans, Sang‐Moo Kang
ABSTRACTA simple method suitable for self-administration of vaccine would improve mass immunization, particularly during a pandemic outbreak. Influenza virus-like particles (VLPs) have been suggested as promising vaccine candidates against potentially pandemic influenza viruses, as they confer long-lasting immunity but are not infectious. We investigated the immuno...... hiện toàn bộ
Use of an Inhibition Enzyme-Linked Immunosorbent Assay for Quantification of Capsular Polysaccharide or Proteins in Vaccines
American Society for Microbiology - Tập 14 Số 3 - Trang 323-327 - 2007
Thomas J. Inzana, Anna E. Champion
ABSTRACTAn inhibition enzyme-linked immunosorbent assay (ELISA) is described for quantification of capsular polysaccharide or proteins in vaccines and other samples containing whole cells or extracts ofActinobacillus pleuropneumoniae. The assay can be used to quantify any antigen that can be purified and for which highly specific antibodi...... hiện toàn bộ
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