American Society for Microbiology

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Acute Inflammatory Response to Endotoxin in Mice and Humans
American Society for Microbiology - Tập 12 Số 1 - Trang 60-67 - 2005
Shannon Copeland, H. Shaw Warren, Stephen F. Lowry, Steve E. Calvano, Daniel G. Remick
ABSTRACTEndotoxin injection has been widely used to study the acute inflammatory response. In this study, we directly compared the inflammatory responses to endotoxin in mice and humans.Escherichia colitype O113 endotoxin was prepared under identical conditions, verified to be of equal biological potency, and used for both mice and humans...... hiện toàn bộ
Human Immunodeficiency Virus Persistence and Production in T-Cell Development
American Society for Microbiology - Tập 13 Số 11 - Trang 1237-1245 - 2006
Kevin B. Gurney, Christel H. Uíttenbogaart
ABSTRACTHuman immunodeficiency virus type 1 (HIV-1) replication depends on CD4 and coreceptor expression as well as host factors associated with the activation state of the cell. To determine the impact of the activation stage of thymocytes on the HIV-1 life cycle, we investigated R5 and X4 HIV-1 entry, reverse transcription, and expression in discrete thymocyte su...... hiện toàn bộ
Optimization and Validation of a Multiplex, Electrochemiluminescence-Based Detection Assay for the Quantitation of Immunoglobulin G Serotype-Specific Antipneumococcal Antibodies in Human Serum
American Society for Microbiology - Tập 16 Số 3 - Trang 387-396 - 2009
Rocio D. Marchese, Derek Puchalski, Pamela Miller, Joseph Antonello, Olivia Hammond, Tina Green, Leonard J. Rubinstein, Michael J. Caulfield, Daniel J. Sikkema
ABSTRACT Pneumovax 23 consists of a mixture of highly purified capsular polysaccharides (Ps) from 23 of the most prevalent serotypes of Streptococcus pneumoniae . Testing of vaccine immunogenicity has been historically performed on the enzyme-linked immunosorbent assay (ELISA) platform, valid...... hiện toàn bộ
Use of Opsonophagocytosis for Serological Evaluation of Pneumococcal Vaccines
American Society for Microbiology - Tập 13 Số 2 - Trang 165-169 - 2006
Sandra Romero‐Steiner, Carl E. Frasch, George M. Carlone, Roland A. Fleck, David Goldblatt, Moon H. Nahm
Differentiation, Maturation, and Survival of Dendritic Cells by Osteopontin Regulation
American Society for Microbiology - Tập 12 Số 1 - Trang 206-212 - 2005
Kodai Kawamura, Kazuhiro Iyonaga, Hidenori Ichiyasu, Junko Nagano, Moritaka Suga, Yutaka Sasaki
ABSTRACTDendritic cells (DCs) are antigen-presenting cells with the ability to induce primary immune responses necessary in innate immunity and adaptive immunity. Osteopontin (OPN) is a secreted acidic phosphoprotein containing an arginine-glycine-aspartate sequence and has been suggested to play an important role in early cellular immune responses. The interaction...... hiện toàn bộ
Microneedle Delivery of H5N1 Influenza Virus-Like Particles to the Skin Induces Long-Lasting B- and T-Cell Responses in Mice
American Society for Microbiology - Tập 17 Số 9 - Trang 1381-1389 - 2010
Jae-Min Song, Yeu‐Chun Kim, Aleksandr S. Lipatov, Marc Pearton, C. Todd Davis, Dae‐Goon Yoo, Kyoung‐mi Park, Li-Mei Chen, Fu‐Shi Quan, James C. Birchall, Ruben O. Donis, Mark R. Prausnitz, Richard W. Compans, Sang‐Moo Kang
ABSTRACTA simple method suitable for self-administration of vaccine would improve mass immunization, particularly during a pandemic outbreak. Influenza virus-like particles (VLPs) have been suggested as promising vaccine candidates against potentially pandemic influenza viruses, as they confer long-lasting immunity but are not infectious. We investigated the immuno...... hiện toàn bộ
Use of an Inhibition Enzyme-Linked Immunosorbent Assay for Quantification of Capsular Polysaccharide or Proteins in Vaccines
American Society for Microbiology - Tập 14 Số 3 - Trang 323-327 - 2007
Thomas J. Inzana, Anna E. Champion
ABSTRACTAn inhibition enzyme-linked immunosorbent assay (ELISA) is described for quantification of capsular polysaccharide or proteins in vaccines and other samples containing whole cells or extracts ofActinobacillus pleuropneumoniae. The assay can be used to quantify any antigen that can be purified and for which highly specific antibodi...... hiện toàn bộ
Flexible Label-Free Quantitative Assay for Antibodies to Influenza Virus Hemagglutinins
American Society for Microbiology - Tập 17 Số 9 - Trang 1407-1416 - 2010
P.J. Carney, Aleksandr S. Lipatov, Arnold S. Monto, Ruben O. Donis, James Stevens
ABSTRACT During the initial pandemic influenza H1N1 virus outbreak, assays such as hemagglutination inhibition and microneutralization provided important information on the relative protection afforded by the population's cross-reactivity from prior infections and immunizations with seasonal vaccines. However, these assays continue to be limited in that t...... hiện toàn bộ
Serologic Cross-Reactivity between Anaplasma marginale and Anaplasma phagocytophilum
American Society for Microbiology - Tập 12 Số 10 - Trang 1177-1183 - 2005
Ute M. Dreher, Not Available Not Available, Regina Hofmann‐Lehmann, Marina L. Meli, Nicola Pusterla, Katherine M. Kocan, Z. Woldehiwet, Ulrike Braun, G. Regula, Katharina D.C. Stärk, Hans Lutz
ABSTRACT In the context of a serosurvey conducted on the Anaplasma marginale prevalence in Swiss cattle, we suspected that a serological cross-reactivity between A. marginale and A. phagocytophilum ...... hiện toàn bộ
Prevalence and Molecular Characterization of Pertactin-Deficient Bordetella pertussis in the United States
American Society for Microbiology - Tập 21 Số 2 - Trang 119-125 - 2014
Lucia C. Pawloski, Anne Marie Queenan, Pamela K. Cassiday, Alisson Lynch, M. J. Harrison, Wenchi Shang, Margaret M. Williams, Katherine E. Bowden, Brunilis Burgos‐Rivera, Xuan Qin, Nancy E. Messonnier, M. Lucia Tondella
ABSTRACTPertussis has shown a striking resurgence in the United States, with a return to record numbers of reported cases as last observed in the 1950s.Bordetella pertussisisolates lacking pertactin, a key antigen component of the acellular pertussis vaccine, have been observed, suggesting that... hiện toàn bộ
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