trans-Cyclohexanediamines Prevent Thermal Inactivation of Protein: Role of Hydrophobic and Electrostatic Interactions
Tóm tắt
Although solution additives prevent protein misfolding, the mechanism remains elusive. In this paper, we compare the preventive effects of trans-1,2-cyclohexanediamine (1,2-CHDA) and trans-1,4-cyclohexanediamine (1,4-CHDA) on the heat-induced inactivation of ribonuclease A (RNase A) and lysozyme. These additives are more effective in preventing thermal inactivation of the proteins than guanidine (Gdn) and arginine (Arg). The results suggest two possibilities: (i) decrease in the hydrophobic interaction between unfolded protein molecules is indispensable for preventing protein association, and (ii) the electrostatic interaction between additives interacting with the hydrophobic residues of protein molecules plays an important role in preventing thermal inactivation of proteins.
Tài liệu tham khảo
Fink AL (1998) Fold Des 3:9–23
Kopito RR (2000) Trends Cell Biol 10:524–530
Tsumoto K, Ejima D, Kumagai I, Arakawa T (2003) Protein Expr Purif 28:1–8
Georgiou G, Valax P, Ostermeier M, Horowitz PM (1994) Protein Sci 3:1953–1960
Winzor CL, Winzor DJ, Paleg LG, Jones GP, Naidu BP (1992) Arch Biochem Biophys 296:102–107
Tsumoto K, Umetsu M, Kumagai I, Ejima D, Philo JS, Arakawa T (2004) Biotechnol Prog 20:1301–1308
Shiraki K, Kudou M, Nishikori S, Kitagawa H, Imanaka T, Takagi M (2004) Eur J Biochem 271:3242–3247
Hirano A, Hamada H, Okubo T, Noguchi T, Higashibata H, Shiraki K (2007) Protein J 26:423–433
Dunbar J, Yennawar HP, Banerjee S, Luo J, Farber GK (1997) Protein Sci 6:1727–1733
Bhuyan AK (2002) Biochemistry 41:13386–13394
Ejima D, Yumioka R, Tsumoto K, Arakawa T (2005a) Anal Biochem 345:250–257
Ejima D, Yumioka R, Arakawa T, Tsumoto K (2005b) J Chromatogr A 1094:49–55
Tsumoto K, Shinoki K, Kondo H, Uchikawa M, Juji T, Kumagai I (1998) J Immunol Methods 219:119–129
Umetsu M, Tsumoto K, Hara M, Ashish K, Goda S, Adschiri T, Kumagai I (2003) J Biol Chem 278:8979–8987
Ishibashi M, Tsumoto K, Ejima D, Arakawa T, Tokunaga M (2005) Protein Pept Lett 12:649–653
Chow MK, Amin AA, Fulton KF, Whisstock JC, Buckle AM, Bottomley SP (2006) Protein Expr Purif 46:166–171
Ejima D, Ono K, Tsumoto K, Arakawa T, Eto Y (2006) Protein Expr Purif 47:45–51
Shiraki K, Kudou M, Fujiwara S, Imanaka T, Takagi M (2002) J Biochem (Tokyo) 132:591–595
Arakawa T, Tsumoto K (2003) Biochem Biophys Res Commun 304:148–152
Kudou M, Shiraki K, Fujiwara S, Imanaka T, Takagi M (2003) Eur J Biochem 270:4547–4554
Okanojo M, Shiraki K, Kudou M, Nishikori S, Takagi M (2005) J Biosci Bioeng 100:556–561
Shiraki K, Kudou M, Sakamoto R, Yanagihara I, Takagi M (2005) Biotechnol Prog 21:640–643
Hamada H, Shiraki K (2007) J Biotechnol 130:153–160
Matsuoka T, Tomita S, Hamada H, Shiraki K (2007) J Biosci Bioeng 103:440–443