miRNA analysis in pancreatic cancer: the Dartmouth experience

Clinical Chemistry and Laboratory Medicine - Tập 55 Số 5 - 2017
Francine B. de Abreu1,2, Xiaoying Liu1,2, Gregory J. Tsongalis3
1Department of Pathology and Laboratory Medicine, Dartmouth Hitchcock Medical Center and Norris Cotton Cancer Center, Lebanon, NH, USA
2The Theodore and Audrey Geisel School of Medicine at Dartmouth, Hanover, NH, USA
3Department of Pathology, Dartmouth Hitchcock Medical Center, Lebanon, NH 03756, USA, Phone: 603-650-5498

Tóm tắt

Abstract:Pancreatic cancer is considered one of the most lethal cancers being the fourth leading cause of cancer deaths in adults in the United States because of the lack of early signs and symptoms and the lack of early detection. Pancreatic ductal adenocarcinoma (PDAC) is the most common histological type among pancreatic cancers, representing 80%–90% of all solid tumors of the pancreas. The majority of PDAC develops from three precursor lesions: pancreatic intraepithelial neoplasia, intraductual papillary mucinous neoplasm and mucinous cystic neoplasm. Although histologic tissue evaluation remains the gold standard for diagnosis, endoscopic ultrasound-guided fine needle aspiration has become the preferred modality for obtaining pathologic confirmation. At Dartmouth-Hitchcock Medical Center (DHMC),we have developed and validated a microRNA (miRNA) panel for patients with pancreatic diseases that can be used in association with the gold standard method for diagnosis. miRNAs have an important role in biological processes, such as apoptosis, metabolism, cell growth and differentiation. In cancer, miRNAs can be classified as either oncogenic or tumor suppressor according to their function in the carcinogenic process. In this study, we describe the expression of many miRNA in benign and malignant pancreatic tissues as well as their clinical significance. For this reason, miRNAs have been considered potential biomarkers of pancreatic diseases that could potentially contribute to an early diagnosis, predict disease progression, accurately monitor disease, contribute to better treatment strategies and reduce mortality by improving disease management.

Từ khóa


Tài liệu tham khảo

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2007, In: Atlas of Tumor Pathology

1998, Progression of pancreatic intraductal neoplasias to infiltrating adenocarcinoma of the pancreas, Am J Surg Pathol, 22, 163, 10.1097/00000478-199802000-00003

2009, Preoperative tissue diagnosis for tumours of the pancreas, Br J Surg, 96, 5, 10.1002/bjs.6407

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2017, MicroRNA in pancreatic cancer, J Hum Genet, 62, 33, 10.1038/jhg.2016.59

2015, Contribution of microRNA analysis to characterisation of pancreatic lesions: a review, J Clin Pathol, 68, 859, 10.1136/jclinpath-2015-203246

2014, A pilot study to develop a diagnostic test for pancreatic ductal adenocarcinoma based on differential expression of select miRNA in plasma and bile, Am J Gastroenterol, 109, 1942, 10.1038/ajg.2014.331

2001, Pancreatic intraepithelial neoplasia: a new nomenclature and classification system for pancreatic duct lesions, Am J Surg Pathol, 25, 579, 10.1097/00000478-200105000-00003

2015, MicroRNA signaling pathway network in pancreatic ductal adenocarcinoma, Sun J Genet Genomics, 42, 563, 10.1016/j.jgg.2015.07.003

2013, Treatment of HCV infection by targeting microRNA, N Engl J Med, 368, 1685, 10.1056/NEJMoa1209026

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2012, MicroRNA profiling: approaches and considerations, Nat Rev Genet, 13, 358, 10.1038/nrg3198

2013, Endoscopically acquired pancreatic cyst fluid microRNA 21 and 221 are associated with invasive cancer, Am J Gastroenterol, 108, 1352, 10.1038/ajg.2013.167

2009, Preoperative tissue diagnosis for tumours of the pancreas, Br J Surg, 96, 5, 10.1002/bjs.6407

2007, microRNAs as oncogenes and tumor suppressors, Dev Biol, 302, 1, 10.1016/j.ydbio.2006.08.028

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2009, MicroRNA miR-34 inhibits human pancreatic cancer tumor-initiating cells, PLoS One, 4, e6816, 10.1371/journal.pone.0006816

2010, The silencing of microRNA 148a production by DNA hypermethylation is an early event in pancreatic carcinogenesis, Clin Chem, 56, 1107, 10.1373/clinchem.2010.144709

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Clinical Chemistry and Laboratory Medicine

Tập 55 Số 5

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