miR-326 regulates HbF synthesis by targeting EKLF in human erythroid cells

Sankaran, 2013, The switch from fetal to adult hemoglobin, Cold Spring Harb Perspect Med, 3, a11643, 10.1101/cshperspect.a011643 Siatecka, 2011, The multifunctional role of EKLF/KLF1 during erythropoiesis, Blood, 118, 2044, 10.1182/blood-2011-03-331371 Zhou, 2010, KLF1 regulates BCL11A expression and gamma- to beta-globin gene switching, Nat Genet, 42, 742, 10.1038/ng.637 Borg, 2010, Haploinsufficiency for the erythroid transcription factor KLF1 causes hereditary persistence of fetal hemoglobin, Nat Genet, 42, 801, 10.1038/ng.630 Perkins, 1996, Silencing of human fetal globin expression is impaired in the absence of the adult beta-globin gene activator protein EKLF, Proc Natl Acad Sci USA, 93, 12267, 10.1073/pnas.93.22.12267 Magor, 2015, KLF1-null neonates display hydrops fetalis and a deranged erythroid transcriptome, Blood, 125, 2405, 10.1182/blood-2014-08-590968 Liu, 2014, KLF1 mutations are relatively more common in a thalassemia endemic region and ameliorate the severity of beta-thalassemia, Blood, 124, 803, 10.1182/blood-2014-03-561779 Satta, 2011, Compound heterozygosity for KLF1 mutations associated with remarkable increase of fetal hemoglobin and red cell protoporphyrin, Haematologica, 96, 767, 10.3324/haematol.2010.037333 Southwood, 1996, Erythroid Kruppel-like factor exhibits an early and sequentially localized pattern of expression during mammalian erythroid ontogeny, Dev Dyn, 206, 248, 10.1002/(SICI)1097-0177(199607)206:3<248::AID-AJA3>3.0.CO;2-I Li, 2012, Characterization of the transcriptome profiles related to globin gene switching during in vitro erythroid maturation, BMC Genomics, 13, 153, 10.1186/1471-2164-13-153 Cui, 2009, Chromatin signatures in multipotent human hematopoietic stem cells indicate the fate of bivalent genes during differentiation, Cell Stem Cell, 4, 80, 10.1016/j.stem.2008.11.011 Frontelo, 2007, Novel role for EKLF in megakaryocyte lineage commitment, Blood, 110, 3871, 10.1182/blood-2007-03-082065 Perreault, 2017, Epo reprograms the epigenome of erythroid cells, Exp Hematol, 51, 47, 10.1016/j.exphem.2017.03.004 Chen, 1998, Chromatin structure and transcriptional control elements of the erythroid Kruppel-like factor (EKLF) gene, J Biol Chem, 273, 25031, 10.1074/jbc.273.39.25031 Lohmann, 2008, Activation of Eklf expression during hematopoiesis by Gata2 and Smad5 prior to erythroid commitment, Development, 135, 2071, 10.1242/dev.018200 Bianchi, 2010, c-myb supports erythropoiesis through the transactivation of KLF1 and LMO2 expression, Blood, 116, e99, 10.1182/blood-2009-08-238311 Yien, 2015, Alternative splicing of EKLF/KLF1 in murine primary erythroid tissues, Exp Hematol, 43, 65, 10.1016/j.exphem.2014.08.007 Noh, 2009, Let-7 microRNAs are developmentally regulated in circulating human erythroid cells, J Transl Med, 7, 98, 10.1186/1479-5876-7-98 Gabbianelli, 2010, Mechanism of human Hb switching: a possible role of the kit receptor/miR 221–222 complex, Haematologica, 95, 1253, 10.3324/haematol.2009.018259 Sankaran, 2011, MicroRNA-15a and -16-1 act via MYB to elevate fetal hemoglobin expression in human trisomy 13, Proc Natl Acad Sci USA, 108, 1519, 10.1073/pnas.1018384108 Lulli, 2013, MicroRNA-486-3p regulates gamma-globin expression in human erythroid cells by directly modulating BCL11A, PLoS ONE, 8, e60436, 10.1371/journal.pone.0060436 Azzouzi, 2011, MicroRNA-96 directly inhibits gamma-globin expression in human erythropoiesis, PLoS ONE, 6, e22838, 10.1371/journal.pone.0022838 Donze, 1995, Role of erythroid Kruppel-like factor in human gamma- to beta-globin gene switching, J Biol Chem, 270, 1955, 10.1074/jbc.270.4.1955 Viprakasit, 2014, Mutations in Kruppel-like factor 1 cause transfusion-dependent hemolytic anemia and persistence of embryonic globin gene expression, Blood, 123, 1586, 10.1182/blood-2013-09-526087 Ambros, 2004, The functions of animal microRNAs, Nature, 431, 350, 10.1038/nature02871 He, 2004, MicroRNAs: small RNAs with a big role in gene regulation, Nat Rev Genet, 5, 522, 10.1038/nrg1379 Bartel, 2004, MicroRNAs: genomics, biogenesis, mechanism, and function, Cell, 116, 281, 10.1016/S0092-8674(04)00045-5 Carrington, 2003, Role of microRNAs in plant and animal development, Science, 301, 336, 10.1126/science.1085242 Kim, 2004, Identification of many microRNAs that copurify with polyribosomes in mammalian neurons, Proc Natl Acad Sci USA, 101, 360, 10.1073/pnas.2333854100 Du, 2009, MicroRNA miR-326 regulates TH-17 differentiation and is associated with the pathogenesis of multiple sclerosis, Nat Immunol, 10, 1252, 10.1038/ni.1798 Wang, 2015, Prediction of non-small cell lung cancer metastasis-associated microRNAs using bioinformatics, Am J Cancer Res, 5, 32 Sebastiani, 2011, Increased expression of microRNA miR-326 in type 1 diabetic patients with ongoing islet autoimmunity, Diabetes Metab Res Rev, 27, 862, 10.1002/dmrr.1262 Das, 2014, MicroRNA-326 regulates profibrotic functions of transforming growth factor-beta in pulmonary fibrosis, Am J Respir Cell Mol Biol, 50, 882, 10.1165/rcmb.2013-0195OC Kefas, 2009, The neuronal microRNA miR-326 acts in a feedback loop with notch and has therapeutic potential against brain tumors, J Neurosci, 29, 15161, 10.1523/JNEUROSCI.4966-09.2009 Baek, 2008, The impact of microRNAs on protein output, Nature, 455, 64, 10.1038/nature07242 Selbach, 2008, Widespread changes in protein synthesis induced by microRNAs, Nature, 455, 58, 10.1038/nature07228 Huang, 2015, Compound heterozygosity for KLF1 mutations is associated with microcytic hypochromic anemia and increased fetal hemoglobin, Eur J Hum Genet, 23, 1341, 10.1038/ejhg.2014.291 Zhou, 2006, Differential binding of erythroid Krupple-like factor to embryonic/fetal globin gene promoters during development, J Biol Chem, 281, 16052, 10.1074/jbc.M601182200 Drissen, 2004, The active spatial organization of the beta-globin locus requires the transcription factor EKLF, Genes Dev, 18, 2485, 10.1101/gad.317004