hB1F and HNF1 work synergistically in the regulation of the function of HBV EN II
Tóm tắt
Human B1 binding factor (hB1F) is a novel transcription factor cloned in our laboratory, which has the capacity of binding the Bl region of HBV enhancer II (EN II) and stimulating its activity. In this report, the functional relationship between hB1F and other liver-enriched activators of EN II was investigated. The results of CAT assay indicated that there was only additive effect between hB1F and HNF4 as well as HNF3β. On the contrary, the presence of both hB1F and HNF1 would result in significant functional synergism. Further GST pull down assay indicated that hB1F could interact with HNF1 directlyin vitro. This study provides the evidence that hB1F and HNF1 work synergistically in the regulation of the activity of HBV EN II.
Tài liệu tham khảo
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