Các đoạn cDNA và Phân Tích Biểu Hiện của cpHSC70 và mtHSC70 trong Cải Ngọn Không Đắm

Plant Molecular Biology Reporter - Tập 32 - Trang 531-540 - 2013
Hongbing Song1, Xiaoming Song1, Huanhuan Liu1, Tongkun Liu1, Ying Li1, Xilin Hou1
1State Key Laboratory of Crop Genetics and Germplasm Enhancement/Key Laboratory of Biology and Germplasm Enhancement of Horticultural Crops in East China, Ministry of Agriculture, Nanjing Agricultural University, Nanjing, China

Tóm tắt

Protein sốc nhiệt 70 kDa (HSP70) là cần thiết cho khả năng chịu nhiệt và phát triển ở thực vật cũng như các sinh vật khác. Chúng tôi đã áp dụng các kỹ thuật sao chép dựa trên đồng đồng gen và PCR phiên mã ngược định lượng thời gian thực để xác định chức năng của BccpHSC70s và BcmtHSC70s - các protein tương đồng sốc nhiệt (HSCs) nằm trong chất diệp lục và ti thể, tương ứng - trong cải ngọn không đắm (Brassica campestris ssp. chinensis). Các đoạn cDNA của BccpHSC70s và BcmtHSC70s có sự đồng hình cao với nhau và với các đoạn từ các loài thực vật khác. Không giống như các HSP70 khác, BccpHSC70-1 chỉ có hai motif đặc trưng (ba motif cổ điển được tìm thấy trong chuỗi amino acid được dự đoán của HSP70, nhưng không phải tất cả HSP70 đều chứa cả ba miền), và được biểu hiện liên tục trong ba giống đã phát hiện; biểu hiện của BccpHSC70-1 trong một giống chịu nhiệt (NHCC001) cao hơn so với các giống khác (NHCC002 và NHCC004) trong quá trình phát triển. Mức độ biểu hiện cao của BccpHSC70-2 trong lá có thể được giải thích bởi thực tế rằng các chất diệp lục nhạy cảm với căng thẳng nhiệt, và gen này có thể là một ứng cử viên cho việc phát triển các giống cải ngọn không đắm có khả năng chịu nhiệt. Biểu hiện của BcmtHSC70-2 có thể được kích thích dưới căng thẳng nhiệt. Biểu hiện của BcHSC70s trong dòng vô trùng cho thấy rằng nó đóng một vai trò quan trọng trong quá trình chết tế bào trong sự phát triển của các cơ quan sinh sản.

Từ khóa

#HSP70 #BccpHSC70s #BcmtHSC70s #cải ngọn không đắm #khả năng chịu nhiệt #phát triển cơ quan sinh sản

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