cAMP activates hyperpolarization-activated Ca2+ channels in the pollen of Pyrus pyrifolia

Plant Cell Reports - Tập 30 - Trang 1193-1200 - 2011
Juyou Wu1, Haiyong Qu1, Cong Jin1, Zhongling Shang2, Jun Wu1, Guohua Xu1, Yongbing Gao1, Shaoling Zhang1
1State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Horticulture, Nanjing Agricultural University, Nanjing, China
2College of Life Sciences, HeBei Normal University, Shi Jia Zhuang, China

Tóm tắt

Many signal-transduction processes in plant cells have been suggested to be triggered by signal-induced opening of calcium ion (Ca2+) channels in the plasma membrane. Cyclic nucleotides have been proposed to lead to an increase in cytosolic free Ca2+ in pollen. However, direct recordings of cyclic-nucleotide-induced Ca2+ currents in pollen have not yet been obtained. Here, we report that cyclic AMP (cAMP) activated a hyperpolarization-activated Ca2+ channel in the Pyrus pyrifolia pollen tube using the patch-clamp technique, which resulted in a significant increase in pollen tube protoplast cytosolic-Ca2+ concentration. Outside-out single channel configuration identified that cAMP directly increased the Ca2+ channel open-probability without affecting channel conductance. cAMP-induced currents were composed of both Ca2+ and K+. However, cGMP failed to mimic the cAMP effect. Higher cytosolic free-Ca2+ concentration significantly decreased the cAMP-induced currents. These results provide direct evidence for cAMP activation of hyperpolarization-activated Ca2+ channels in the plasma membrane of pollen tubes, which, in turn, modulate cellular responses in regulation of pollen tube growth.

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