Wheat transcription factor TaWRKY70 is positively involved in high‐temperature seedling plant resistance to Puccinia striiformis f. sp. tritici

Molecular Plant Pathology - Tập 18 Số 5 - Trang 649-661 - 2017
Junjuan Wang1, Fei Tao1, Fei An1, Yiping Zou1, Wei Tian1, Xianming Chen2, Xiangming Xu3, Xiaoping Hu1
1State Key Laboratory of Crop Stress Biology for Arid Areas and College of Plant Protection, Northwest A&F University, Taicheng Road 3, Yangling, Shaanxi, 712100 China
2Agricultural Research Service, United States Department of Agriculture and Department of Plant Pathology Washington State University Pullman WA 99164–6430 USA
3East Malling Research, New Road, East Malling, Kent ME19 6BJ, UK

Tóm tắt

SummaryStripe rust, caused by Puccinia striiformis f. sp. tritici (Pst), is a devastating disease of wheat (Triticum aestivum) worldwide. Wheat high‐temperature seedling plant (HTSP) resistance to Pst is non‐race‐specific and durable. WRKY transcription factors have been proven to play important roles in plant defence responses to attacks by several pathogens. However, there is no direct evidence as to whether WRKY transcription factors play a role in HTSP resistance to Pst. We isolated a WRKY gene, named TaWRKY70, from wheat cultivar Xiaoyan 6. The expression level of TaWRKY70 was increased significantly when exposed to high temperatures (HTs) during the initial symptom expression stage of Pst infection. The expression of this gene increased in plants treated with ethylene (ET), salicylic acid (SA) and cold (4°C) stresses, but decreased in plants treated with methyl jasmonate (MeJA) and heat (40°C) stresses. Silencing of TaWRKY70 led to greater susceptibility to Pst (in terms of the increase in length of uredinial pustules and the decrease in the number of necrotic cells) compared with non‐silenced plants when exposed to HT during the initial symptom expression stage of Pst infection, coinciding with expression changes of the ET‐ and SA‐responsive genes TaPIE1 and TaPR1.1. In contrast, the expression level of the jasmonic acid (JA)‐responsive gene TaAOS was not affected by TaWRKY70. These results indicate that TaWRKY70 is positively involved in HTSP resistance, during which SA and ET signalling are probably activated.

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Molecular Plant Pathology

Tập 18 Số 5

649-661

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