Vitamins determination by TLC/HPTLC—a mini-review
Tóm tắt
This review describes the application of thin-layer chromatography (TLC) and high-performance thin-layer chromatography (HPTLC) in the separation and identification of vitamins from their mixtures using different kinds of mobile phases such as isocratic and mixed solvents as well as mobile phases containing various modifiers. TLC has long been used for the separation and semiquantitative determination of biomolecules including vitamins. HPTLC is the modified version of TLC, and it offers the advantages of better resolution, smaller sample size, and lesser volumes of mobile phases. In recent years, the emergence of advanced and fully automated chromatographic techniques such as high-performance liquid chromatography (HPLC) and gas chromatography (GC) has reduced the application of TLC/HPTLC for vitamins analysis. However, due to the inherent simplicity and cost-effectiveness, both TLC and HPTLC continue to be the methods of choice in small laboratories of developing countries. In this short review, the literature survey on TLC/HPTLC of vitamins covering the period 2011‒2019 has been encapsulated in a tabular form.
Tài liệu tham khảo
Mohammad A, Moheman A (2011) TLC/HPTLC in biomedical applications. In: Srivastava MM (ed) High-performance thin-layer chromatography (HPTLC). Springer, Heidelberg, pp 151–178
Mohammad A, Moheman A, EI-Desoky GE (2012) Amino acid and vitamin determination by TLC/HPTLC: review of the current state. Cent Eur J Chem 10:731–775. https://doi.org/10.2478/s11532-012-0019-0
Pyka A (2014) Detection progress of selected drugs in TLC: a review. Bio Med Res Int 2014:1–19. https://doi.org/10.1155/2014/732078
Zhang Y, Zhou W, Yan JQ, Liu M, Zhou Y, Shen X, Ma YL, Feng XS, Yang J, Li GH (2018) A review of the extraction and determination methods of thirteen essential vitamins to human body: an update from 2010. Molecules 23:1484–1509. https://doi.org/10.3390/molecules23061484
Fatima Z, Jin X, Zhou Y, Kaw HY, Quinto M, Li D (2019) Recent trends in analytical methods for water soluble vitamins. J Chromatogr A 1606:1–18. https://doi.org/10.1016/j.chroma.2019.05.025
Sobanska AW (2019) Impregnated silica-based layers in thin-layer chromatography. J Liq Chromatogr Relat Technol 43:319–327. https://doi.org/10.1080/10826076.2020.1725554
Cimpoiu C, Hosu A, Pusca A (2012) Thin-layer chromatography with stationary gradient phase as a method for water soluble vitamins. J Chromatogr A 1223:142–146. https://doi.org/10.1016/j.chroma.2011.12.021
Dzema D, Kartosova L, Kapizova D, Tripp S, Polikarpov N, Appelhans D, Voit B (2016) Oligosacchrides-crowned hyperbranched poly(ethyleneimine) as an additive to thin-layer chromatography system for the separation of vitamins, amino acids and β- blocker enantiomers. J Planar Chromatogr 29:108–112. https://doi.org/10.1556/1006.2016.29.2.3
Panahi HA, Kalal HS, Rahimi A, Moniri E (2011) Isolation and quantitative analysis of B1, B2, B6 and B12 vitamin using high-performance thin layer chromatography. Pharm Chem J 45:125–129
Demchenko DV, Pozharitskaya ON, Shikov AN, Makarov GV (2011) Validated HPTLC method for quantification of vitamin D3 in fish oil. J Planar Chromatogr 24:487–490. https://doi.org/10.1556/JPC.24.2011.6.6
Kondawar MS, Kamble KG, Mali DS (2011) Quantitative estimation of gallic acid and ascorbic acid in a marketed herbal medicine: Triphila churana by high-performance thin layer chromatography. Int J Pharm Tech Res 3:1593–1599
Atia NN, Ahmad S (2012) A validated high-throughput chromatographic method for simultaneous determination of vitamin K homologues. J Liq Chromatogr Relat Technol 35:484–498
Mohammad A, Siddiq A, Moheman A, EI-Desoky GE (2013) Green analytical method for identification of amino acids, vitamins, sugars with preliminary separation on HPTLC plates. Indian J Chem Technol 20:180–184
Al-Taweel AM, Alqasoumi S, Alam P, Abdel-Kader MS (2013) Densitometric high-performance thin-layer chromatographic estimation of diosmin, hesperidin and ascorbic acid in pharmaceutical formulations. J Planar Chromatogr 26:336–342. https://doi.org/10.1556/JPC.26.2013.4.8
Hima V, Sadasivam RK, Dugnath N, Devanna N (2013) Quantization of ascorbic acid in ayurvedic amla by various analytical techniques. Pharma Chem 5:8–17
Younes KM, Basha MA, Salem MY (2014) Spectrophotometric and chromatographic methods for simultaneous determination of rutin and ascorbic acid in their pharmaceutical formulation. Pharma Chem 6:111–121
Dowoly M, Pyka A (2015) Validation of an RPHPTLC densitometric method using silica gel 60 RP 18WF254 for simultaneous determination of nicotinamide in selected pharmaceutical formulations. J Anal Methods Chem 2015:1–9. https://doi.org/10.1155/2015/631025
Riswanto FDO, Lukitaningsi PRE, Martono S (2015) Analytical method validation and determination of pyridoxine, nicotinamide and caffeine in energy drinks using thin-layer chromatography-densitometry. Indones J Chem 15:9–15
Abdelrahman MM, Abdelwahab NS, Salma FM, Ahmed AB (2016) Different chromatographic methods for determination of vitamin E and vinpocetine in their combined dosage form and in the presence of the alkaline-induced degradation product of vinpocetin dosage. J Planar Chromatogr 29:372–379. https://doi.org/10.1556/1006.2016.29.5.8
Hosu A, Cimpoiu C (2017) Thin-layer chromatography applied in quality assessment of beverages derived from fruits. J Liq Chromatogr Relat Technol 40:239–246. https://doi.org/10.1080/10826076.2017.1298025
Kulkarni MB, Joshi AM, Patil RV (2018) A novel HPTLC method for simultaneous determination of co-enzyme Q10 and α-tocopherol in bulk and pharmaceutical formulation. Int J Pharm Sci 10:134–141. https://doi.org/10.22159/ijpps.2018v10i10.28828
Ali NW, Abdelwahab NS, Abdelrahman MM, EL-Zeiny BA, Tohamy SY (2018) Development and validation of spectrophotometric and high-performance thin-layer chromatographic methods for determination of folic acid in the presence of its impurities (degradation products). J Planar Chromatogr 31:367–376. https://doi.org/10.1556/1006.2018.31.5.4
Abdelwahab NS, Abdelaleem EA, Abdelrahman MM (2019) HPTLC-densitometric determination method for determination of ascorbic acid, paracetamol and guaifenesin in presence of their toxic impurities. J Chromatogr Sci 57:149–155. https://doi.org/10.1093/chromsci/bmy096
Alam P, Kamal YT, Alqasoumi SI, Foudha AI, Alqarmi MH, Yosufoglu HS (2019) HPTLC method for determination of ascorbic acid and gallic acid biomarker from freeze dry pomegranatejuice and herbal formulation. Saudi Pharm J 27:975–980. https://doi.org/10.1016/j.jsps.2019.07.006
Peter E, Kaligirwa A, Lukwago TW (2019) Stability indicating high-performance thin-layer chromatography method for estimation of ascorbic acid in Hibiscus sabdariffa L. aqueous extract. Complement Med Res 10:50–57. https://doi.org/10.5455/jcmr.20181211114301