Hình ảnh hóa các tế bào T cytotoxic đặc hiệu kháng nguyên ở người được gán nhãn bằng hạt oxit sắt siêu từ tính

European Radiology - Tập 18 - Trang 1087-1095 - 2008
Ambros J. Beer1, Konstantin Holzapfel2, Juliana Neudorfer3, Guido Piontek4, Marcus Settles2, Holger Krönig3, Christian Peschel3, Jürgen Schlegel4, Ernst J. Rummeny2, Helga Bernhard3
1Department of Nuclear Medicine, Klinikum rechts der Isar, Technical University of Munich (TUM), Munich, Germany
2Department of Radiology, Klinikum Rechts der Isar, Technical University of Munich, Munich, Germany
3Department of Hematology/Oncology, Klinikum rechts der Isar, TUM, Munich, Munich, Germany
4Division of Neuropathology, Institute of Pathology, Klinikum rechts der Isar, TUM, Munich, Munich, Germany

Tóm tắt

Các công nghệ mới cần thiết để đánh giá sự di động và khả năng sống sót của các tế bào T đặc hiệu kháng nguyên trong cơ thể sống. Trong nghiên cứu này, chúng tôi đã phát triển một kỹ thuật mới để gán nhãn các tế bào T lymphocyte cytotoxic ở người bằng hạt oxit sắt siêu từ tính và sau đó sử dụng máy quét cộng hưởng từ 1.5-T thông thường để hình dung. Các tế bào lymphocyte T CD8+ đặc hiệu kháng nguyên đã được gán nhãn bằng ferucarbotran thông qua lipofection. Việc hấp thụ ferucarbotran đã được xác nhận bằng vi kính huỳnh quang sử dụng kháng thể đặc hiệu dextran, và sự tích tụ sắt bên trong tế bào đã được đo bằng quang phổ hấp thụ nguyên tử. Việc hình ảnh hóa các tế bào T được thực hiện bằng cộng hưởng từ vào ngày 0, 2, 7 và 14 sau quy trình gán nhãn. Vào ngày 0 và 2 sau khi gán nhãn, chúng tôi đã quan sát thấy sự rút ngắn rõ rệt thời gian thư giãn T2*-thời gian, điều này đã giảm sau 7 ngày và không thể phát hiện được nữa sau 14 ngày, có thể là do hoạt động phân chia của các tế bào T đã được gán nhãn. Quan trọng là, hoạt động ly giải đặc hiệu kháng nguyên của các tế bào T vẫn được duy trì sau khi gán nhãn ferucarbotran. Việc gán nhãn hiệu quả ferucarbotran cho các tế bào T lymphocyte hoạt động chức năng và sự phát hiện của chúng qua hình ảnh cộng hưởng từ cho phép việc theo dõi các tế bào T trong cơ thể sống và, sau đó, sẽ ảnh hưởng đến sự phát triển tiếp theo của các liệu pháp dựa trên tế bào T.

Từ khóa

#tế bào T đặc hiệu kháng nguyên #hình ảnh cộng hưởng từ #hạt oxit sắt siêu từ tính #ferucarbotran #T lymphocyte cytotoxic

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