Verrucarin A Inhibition of MAP Kinase Activation in a PMA-stimulated Promyelocytic Leukemia Cell Line

Marine Drugs - Tập 3 Số 2 - Trang 64-73
Taiko Oda1, Michio Namikoshi2, Kyoko Akano2, Hisayoshi Kobayashi3, Yoshio Honma4, Tadashi Kasahara5
1Department of Basic Biological Sciences, Kyoritsu College of Pharmacy, Shibakoen 1-chome, 5-30, Minato-ku, Tokyo, 105-8512, Japan
2Department of Ocean Sciences, Tokyo University of Marine Science and Technology, Minato-ku, Tokyo 108-8477, Japan
3Institute of Molecular and Cellular Biosciences, The University of Tokyo, Bunkyo-ku, Tokyo 113- 0032, Japan
4Institute of Radiopharmaceutical Chemistry, Kyoritsu College of Pharmacy, Shibakoen 1-chome, 5-30, Minato-ku, Tokyo, 105-8512, Japan
5Department of Biochemistry, Kyoritsu College of Pharmacy, Shibakoen 1-chome, 5-30, Minato-ku, Tokyo, 105-8512, Japan

Tóm tắt

Verrucarin A is an inhibitor of protein synthesis. In this study, we examined the inhibitory action of verrucarin A on signal molecules. Verrucarin A partially inhibited the IL-8 production of a PMA-stimulated promyelocytic leukemia cell line (HL-60 cells), and the effect was related to the inhibition of NF-κB activation at noncytotoxic concentrations. Moreover, the inhibition of mitogen activated protein (MAP) kinase by verrucarin A was especially strong with p38- and JNK-phosphorylation. The findings show a new action of verrucarin A, and it is expected that this action relaxes the signal activation in response to stress.

Từ khóa


Tài liệu tham khảo

Faulkner, 2002, Marine natural products, Nat. Prod. Rep, 19, 1, 10.1039/b009029h

Hyde, 2002, Secondary metabolites from marine fungi, Fungi In Marine Environments, 7, 293

Liberra, 1995, Marine fungi – a prolific resource of biologically active natural products?, Pharmazie, 50, 583

Kelecom, 2002, Secondary metabolites from marine microorganisms, Annals Brazil. Acad. Sci, 74, 151, 10.1590/S0001-37652002000100012

Proksch, 2002, Drugs from the seas - current status and microbiological implications, Appl. Microbiol. Biotechnol, 59, 125, 10.1007/s00253-002-1006-8

Fusetani, N. (2000). In Drugs from the Sea, Karger.

Fusetani, N. (2000). Drugs from the Sea, Karger.

Pietra, 1997, Secondary metabolites from marine microorganisms: bacteria, protozoa, algae and fungi. Achievements and prospects, Nat. Prod. Rep, 14, 453, 10.1039/np9971400453

Davidson, 1995, New dimensions in natural products research: cultured marine microorganisms, Curr. Opin. Biotechnol, 6, 284, 10.1016/0958-1669(95)80049-2

Namikoshi, 2001, A new macrocyclic trichothecene, 12,13-deoxyroridin E, produced by the marine-derived fungus Myrothecium roridum collected in Palau, J. Nat. Prod, 64, 396, 10.1021/np000443g

Domzig, 1983, Interleukin 2 dependence of human natural killer (NK) cell activity, J. Immunol, 130, 1970, 10.4049/jimmunol.130.4.1970

Kasahara, 1998, Interleukin-8 and monocyte chemotactic protein-1 production by a human glioblastoma cell line, T98G in coculture with monocytes: involvement of monocyte-derived interleukin-1alpha, Eur. Cytokine Netw, 9, 47

Shimizu, 1998, Stimulation of nitric oxide synthase during oxidative endothelial cell injury, Biochem. Pharmacol, 55, 77, 10.1016/S0006-2952(97)00399-7

Carmichael, 1987, Evaluation of a tetrazolium-based semiautomated colorimetric assay: assessment of chemosensitivity testing, Cancer Res, 47, 939

Hiramoto, 1998, Nuclear targeted suppression of NF-kappa B activity by the novel quinone derivative E3330, J. Immunol, 160, 810, 10.4049/jimmunol.160.2.810

Oda, 2003, Inhibition by (±)-indenestrol A of interferon gamma-stimulated nitric oxide formation in murine macrophge RAW 264.7 cells, Mut. Res, 534, 187, 10.1016/S1383-5718(02)00275-9

Mukaida, 1994, Effects of satratoxins and other macrocyclic trichothecenes on IL-2 production and viability of EL-4 thymoma cells, J. Biol. Chem, 269, 13289, 10.1016/S0021-9258(17)36831-X

Lee, M., Li, S., Jarvis, B.B., and Pestka, J.J. (1999). J. Toxicol. Environ. Health A, 57, 459–474.

Li, G., Bishop, K.J., and Hall, T.C. (2001). J. Biol. Chem., 276, 2062–2068.

Thông tin
Thông tin xuất bản

Marine Drugs

Tập 3 Số 2

64-73

Thông tin tác giả