Verrucarin A Inhibition of MAP Kinase Activation in a PMA-stimulated Promyelocytic Leukemia Cell Line
Tóm tắt
Verrucarin A is an inhibitor of protein synthesis. In this study, we examined the inhibitory action of verrucarin A on signal molecules. Verrucarin A partially inhibited the IL-8 production of a PMA-stimulated promyelocytic leukemia cell line (HL-60 cells), and the effect was related to the inhibition of NF-κB activation at noncytotoxic concentrations. Moreover, the inhibition of mitogen activated protein (MAP) kinase by verrucarin A was especially strong with p38- and JNK-phosphorylation. The findings show a new action of verrucarin A, and it is expected that this action relaxes the signal activation in response to stress.
Từ khóa
Tài liệu tham khảo
Hyde, 2002, Secondary metabolites from marine fungi, Fungi In Marine Environments, 7, 293
Liberra, 1995, Marine fungi – a prolific resource of biologically active natural products?, Pharmazie, 50, 583
Kelecom, 2002, Secondary metabolites from marine microorganisms, Annals Brazil. Acad. Sci, 74, 151, 10.1590/S0001-37652002000100012
Proksch, 2002, Drugs from the seas - current status and microbiological implications, Appl. Microbiol. Biotechnol, 59, 125, 10.1007/s00253-002-1006-8
Pietra, 1997, Secondary metabolites from marine microorganisms: bacteria, protozoa, algae and fungi. Achievements and prospects, Nat. Prod. Rep, 14, 453, 10.1039/np9971400453
Davidson, 1995, New dimensions in natural products research: cultured marine microorganisms, Curr. Opin. Biotechnol, 6, 284, 10.1016/0958-1669(95)80049-2
Namikoshi, 2001, A new macrocyclic trichothecene, 12,13-deoxyroridin E, produced by the marine-derived fungus Myrothecium roridum collected in Palau, J. Nat. Prod, 64, 396, 10.1021/np000443g
Domzig, 1983, Interleukin 2 dependence of human natural killer (NK) cell activity, J. Immunol, 130, 1970, 10.4049/jimmunol.130.4.1970
Kasahara, 1998, Interleukin-8 and monocyte chemotactic protein-1 production by a human glioblastoma cell line, T98G in coculture with monocytes: involvement of monocyte-derived interleukin-1alpha, Eur. Cytokine Netw, 9, 47
Shimizu, 1998, Stimulation of nitric oxide synthase during oxidative endothelial cell injury, Biochem. Pharmacol, 55, 77, 10.1016/S0006-2952(97)00399-7
Carmichael, 1987, Evaluation of a tetrazolium-based semiautomated colorimetric assay: assessment of chemosensitivity testing, Cancer Res, 47, 939
Hiramoto, 1998, Nuclear targeted suppression of NF-kappa B activity by the novel quinone derivative E3330, J. Immunol, 160, 810, 10.4049/jimmunol.160.2.810
Oda, 2003, Inhibition by (±)-indenestrol A of interferon gamma-stimulated nitric oxide formation in murine macrophge RAW 264.7 cells, Mut. Res, 534, 187, 10.1016/S1383-5718(02)00275-9
Mukaida, 1994, Effects of satratoxins and other macrocyclic trichothecenes on IL-2 production and viability of EL-4 thymoma cells, J. Biol. Chem, 269, 13289, 10.1016/S0021-9258(17)36831-X