P. Yot1,2, M. Pinck1,2, Anne‐Lise Haenni1,2, Henri Duranton1,2, François Chapeville1,2
1INSTITUT DE BIOLOGIE MOLÉCULAIRE, FACULTÉ DES SCIENCES, 9 QUAI SAINT-BERNARD, PARIS V;
2LABORATOIRE DE PHYSIOLOGIE VÉGÉTALE, INSTITUT DE BOTANIQUE, 8 RUE GOETHE, 67, STRASBOURG, FRANCE
Tóm tắt
The 3′ terminal nucleotide of turnip yellow mosaic virus (TYMV) RNA (23-25 S) may be esterified with valine in the presence of ATP and an enzyme preparation from
Escherichia coli
. The nucleotide composition near the valine-binding site is different for TYMV RNA and tRNA
Val
from cabbage, as shown by comparison of the valine adducts of nucleotides labeled with radioactive valine in T
1
RNase digests. Consequently, host tRNA
Val
is not involved in the observed charging of TYMV RNA with valine. The TYMV RNA appears to have a tRNA-like structure, at or near its 3′ end, that is recognized by three different enzymes which specifically catalyze reactions involving tRNA.
