Val66Met polymorphism in the BDNF gene in children with bronchial asthma

Pediatric Pulmonology - Tập 50 Số 7 - Trang 631-637 - 2015
Miloš Jeseňák1, Eva Babušíková2, Andrea Evinová2, Peter Bánovčin1, Dušan Dobrota2
1Department of Paediatrics, Jessenius Faculty of Medicine in Martin, Comenius University in Bratislava, Martin, Slovakia.
2Department of Medical Biochemistry, Jessenius Faculty of Medicine in Martin, Comenius University in Bratislava, Martin, Slovakia

Tóm tắt

SummaryObjectivesBronchial asthma is a chronic respiratory disease characterized by airway inflammation. There is increasing evidence that neurotrophins play an important role in the development and maintenance of neurogenic airway inflammation in chronic allergic diseases.Working HypothesisBrain‐derived neurotrophic factor (BDNF) is a member of the neurotrophin family and has several important functions in the airways. There are only a few reports on the association between genetic variations in the BDNF gene and various allergic diseases, and the results are generally conflicting. Therefore, we aimed to study the functional polymorphism Val66Met (also called rs6265 or G196A) in the BDNF gene in a group of asthmatic children and healthy controls.Study Design, Patient‐Selection, and MethodologyWe studied 248 asthmatic patients (aged 12.28 ± 0.24 years) and 249 healthy children (aged 13.14 ± 0.48 years). Analysis of the Val66Met polymorphism of the BDNF gene was performed by polymerase chain reaction (PCR) and PCR products were digested by PmlI.ResultsThe prevalence of the Val66Met polymorphisms (Val/Val, Val/Met, and Met/Met) was 61.7%, 33.5%, and 4.8% in asthmatics, respectively, and 47.0%, 51.8%, and 1.2% in healthy subjects, respectively. We observed a significant association of the Met/Met variant genotype with asthmatics (OR = 4.17, 95% CI = 1.16–14.96, P = 0.018). The Val/Met genotype was protective against bronchial asthma (OR = 0.69, 95% CI = 0.48–0.99, P = 0.045), especially in girls (OR = 0.34, 95% CI = 0.20–0.59, P = 0.001).ConclusionSpecific BDNF gene polymorphism may contribute to bronchial asthma susceptibility. Our study suggested the positive association between selected functional BDNF polymorphism (rs6265) and asthma in children. Pediatr Pulmonol. 2015; 50:631–637. © 2014 Wiley Periodicals, Inc.

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Pediatric Pulmonology

Tập 50 Số 7

631-637

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