Using metabolite-responsive gene regulators to improve microbial biosynthesis

Binder, 2012, A high-throughput approach to identify genomic variants of bacterial metabolite producers at the single-cell level, Genome Biol, 13, R40, 10.1186/gb-2012-13-5-r40

Dietrich, 2013, Transcription factor-based screens and synthetic selections for microbial small-molecule biosynthesis, ACS Synth Biol, 2, 47, 10.1021/sb300091d

Reed, 2012, Evolution of an alkane-inducible biosensor for increased responsiveness to short-chain alkanes, J Biotechnol, 158, 75, 10.1016/j.jbiotec.2012.01.028

Xu, 2014, Design and kinetic analysis of a hybrid promoter-regulator system for malonyl-CoA sensing in Escherichia coli, ACS Chem Biol, 9, 451, 10.1021/cb400623m

Zhang, 2012, Design of a dynamic sensor-regulator system for production of chemicals and fuels derived from fatty acids, Nat Biotechnol, 30, 354, 10.1038/nbt.2149

Liu, 2013, Negative feedback regulation of fatty acid production based on a malonyl-CoA sensor–actuator, ACS Synth Biol, 4, 132, 10.1021/sb400158w

Tang, 2011, Design and application of a mevalonate-responsive regulatory protein, Angew Chem Int Ed, 50, 1084, 10.1002/anie.201006083

Tang, 2013, Screening for enhanced triacetic acid lactone production by recombinant Escherichia coli expressing a designed triacetic acid lactone reporter, J Am Chem Soc, 135, 10099, 10.1021/ja402654z

Chen, 2015, Design of an ectoine-responsive AraC mutant and its application in metabolic engineering of ectoine biosynthesis, Metab Eng, 30, 149, 10.1016/j.ymben.2015.05.004

Jha, 2014, Engineering an Acinetobacter regulon for biosensing and high-throughput enzyme screening in E. coli via flow cytometry, Nucleic Acids Res, 42, 8150, 10.1093/nar/gku444

Taylor, 2015, Engineering an allosteric transcription factor to respond to new ligands, Nat Methods, 13, 177, 10.1038/nmeth.3696

Rogers, 2015, Synthetic biosensors for precise gene control and real-time monitoring of metabolites, Nucleic Acids Res, 43, 7648, 10.1093/nar/gkv616

Siedler, 2014, Novel biosensors based on flavonoid-responsive transcriptional regulators introduced into Escherichia coli, Metab Eng, 21, 2, 10.1016/j.ymben.2013.10.011

Berens, 2015, Riboswitch engineering – making the all-important second and third steps, Curr Opin Biotechnol, 31, 10, 10.1016/j.copbio.2014.07.014

Mellin, 2015, Unexpected versatility in bacterial riboswitches, Trends Genet, 31, 150, 10.1016/j.tig.2015.01.005

Rogers, 2016, Biosensor-based engineering of biosynthetic pathways, Curr Opin Biotechnol, 42, 84, 10.1016/j.copbio.2016.03.005

Golynskiy, 2011, Engineering protein switches: sensors, regulators, and spare parts for biology and biotechnology, Chembiochem, 12, 353, 10.1002/cbic.201000642

Stein, 2015, Synthetic protein switches: design principles and applications, Trends Biotechnol, 33, 101, 10.1016/j.tibtech.2014.11.010

Lange, 2011, Lrp of Corynebacterium glutamicum controls expression of the brnFE operon encoding the export system for l-methionine and branched-chain amino acids, J Biotechnol, 158, 231, 10.1016/j.jbiotec.2011.06.003

Mustafi, 2012, The development and application of a single-cell biosensor for the detection of l-methionine and branched-chain amino acids, Metab Eng, 14, 449, 10.1016/j.ymben.2012.02.002

Mahr, 2015, Biosensor-driven adaptive laboratory evolution of l-valine production in Corynebacterium glutamicum, Metab Eng, 32, 184, 10.1016/j.ymben.2015.09.017

Bellmann, 2001, Expression control and specificity of the basic amino acid exporter LysE of Corynebacterium glutamicum, Microbiology, 147, 1765, 10.1099/00221287-147-7-1765

Schendzielorz, 2014, Taking control over control: use of product sensing in single cells to remove flux control at key enzymes in biosynthesis pathways, ACS Synth Biol, 3, 21, 10.1021/sb400059y

Jeong, 2012, High-throughput screening system based on phenolics-responsive transcription activator for directed evolution of organophosphate-degrading enzymes, Protein Eng Des Sel, 25, 725, 10.1093/protein/gzs071

Choi, 2014, Toward a generalized and high-throughput enzyme screening system based on artificial genetic circuits, ACS Synth Biol, 3, 163, 10.1021/sb400112u

Wang, 2016, Design and engineering of intracellular-metabolite-sensing/regulation gene circuits in Saccharomyces cerevisiae, Biotechnol Bioeng, 113, 206, 10.1002/bit.25676

Yang, 2013, Synthetic RNA devices to expedite the evolution of metabolite-producing microbes, Nat Commun, 4, 1413, 10.1038/ncomms2404

Wang, 2015, Evolution of a chimeric aspartate kinase for l-lysine production using a synthetic RNA device, Appl Microbiol Biotechnol, 99, 8527, 10.1007/s00253-015-6615-0

Raman, 2014, Evolution-guided optimization of biosynthetic pathways, Proc Natl Acad Sci U S A, 111, 201409523, 10.1073/pnas.1409523111

Umeyama, 2013, Synthetic gene circuit-mediated monitoring of endogenous metabolites: identification of GAL11 as a novel multicopy enhancer of s-adenosylmethionine level in yeast, ACS Synth Biol, 2, 425, 10.1021/sb300115n

Tang, 2008, AraC regulatory protein mutants with altered effector specificity, J Am Chem Soc, 130, 5267, 10.1021/ja7109053

Frei, 2016, Analysis of amino acid substitutions in AraC variants that respond to triacetic acid lactone, Protein Sci, 25, 804, 10.1002/pro.2873

Gaudu, 1996, SoxR, a [2Fe-2S] transcription factor, is active only in its oxidized form, Proc Natl Acad Sci U S A, 93, 10094, 10.1073/pnas.93.19.10094

Siedler, 2014, SoxR as a single-cell biosensor for NADPH-consuming enzymes in Escherichia coli, ACS Synth Biol, 3, 41, 10.1021/sb400110j

Meyer, 2015, Optimization of a whole-cell biocatalyst by employing genetically encoded product sensors inside nanolitre reactors, Nat Chem, 7, 673, 10.1038/nchem.2301

Schujman, 2003, FapR, a bacterial transcription factor involved in global regulation of membrane lipid biosynthesis, Dev Cell, 4, 663, 10.1016/S1534-5807(03)00123-0

Li, 2015, Development of a synthetic malonyl-CoA sensor in Saccharomyces cerevisiae for intracellular metabolite monitoring and genetic screening, ACS Synth Biol, 4, 1308, 10.1021/acssynbio.5b00069

Xiao, 2016, Exploiting non-genetic, cell-to-cell variation for enhanced biosynthesis, Nat Chem Biol, 12, 339, 10.1038/nchembio.2046

Chou, 2013, Programming adaptive control to evolve increased metabolite production, Nat Commun, 4, 2595, 10.1038/ncomms3595

Lee, 2015, A synthetic suicide riboswitch for the high-throughput screening of metabolite production in Saccharomyces cerevisiae, Metab Eng, 28, 143, 10.1016/j.ymben.2015.01.004

Gong, 2002, Instruction of translating ribosome by nascent peptide, Science, 297, 1864, 10.1126/science.1073997

Fang, 2015, Intermediate-sensor assisted push–pull strategy and its application in heterologous deoxyviolacein production in Escherichia coli, Metab Eng, 33, 41, 10.1016/j.ymben.2015.10.006

Qi, 2013, Repurposing CRISPR as an RNA-guided platform for sequence-specific control of gene expression, Cell, 152, 1173, 10.1016/j.cell.2013.02.022

Nakashima, 2014, A Vector library for silencing central carbon metabolism genes with antisense RNAs in Escherichia coli, Appl Environ Microbiol, 80, 564, 10.1128/AEM.02376-13

Farmer, 2000, Improving lycopene production in Escherichia coli by engineering metabolic control, Nat Biotechnol, 18, 533, 10.1038/75398

Xu, 2014, Improving fatty acids production by engineering dynamic pathway regulation and metabolic control, Proc Natl Acad Sci U S A, 111, 11299, 10.1073/pnas.1406401111

Dahl, 2013, Engineering dynamic pathway regulation using stress-response promoters, Nat Biotechnol, 31, 1039, 10.1038/nbt.2689

Yuan, 2015, Dynamic control of ERG9 expression for improved amorpha-4,11-diene production in Saccharomyces cerevisiae, Microb Cell Fact, 14, 38, 10.1186/s12934-015-0220-x

Soma, 2015, Self-induced metabolic state switching by a tunable cell density sensor for microbial isopropanol production, Metab Eng, 30, 7, 10.1016/j.ymben.2015.04.005

van Ooyen, 2012, Improved l-lysine production with Corynebacterium glutamicum and systemic insight into citrate synthase flux and activity, Biotechnol Bioeng, 109, 2070, 10.1002/bit.24486

Zhou, 2015, Exploring lysine riboswitch for metabolic flux control and improvement of l-lysine synthesis in Corynebacterium glutamicum, ACS Synth Biol, 4, 729, 10.1021/sb500332c

Zhou, 2015, Engineering a lysine-ON riboswitch for metabolic control of lysine production in Corynebacterium glutamicum, ACS Synth Biol, 4, 1335, 10.1021/acssynbio.5b00075

Mukherjee, 2015, GPCR-based chemical biosensors for medium-chain fatty acids, ACS Synth Biol, 4, 1261, 10.1021/sb500365m