Using QIIME to Analyze 16S rRNA Gene Sequences from Microbial Communities

Current Protocols in Bioinformatics - Tập 36 Số 1 - 2011
Justin Kuczynski1, Jesse Stombaugh2, William A. Walters1, Antonio González3, J. Gregory Caporaso4, Rob Knight2
1Department of Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder, Colorado
2Department of Chemistry and Biochemistry, University of Colorado, Boulder, Colorado
3Department of Computer Science, University of Colorado, Boulder, Colorado
4Department of Computer Science, Northern Arizona University, Flagstaff, Arizona

Tóm tắt

AbstractQIIME (canonically pronounced “chime”) is a software application that performs microbial community analysis. It is an acronym for Quantitative Insights Into Microbial Ecology, and has been used to analyze and interpret nucleic acid sequence data from fungal, viral, bacterial, and archaeal communities. The following protocols describe how to install QIIME on a single computer and use it to analyze microbial 16S sequence data from nine distinct microbial communities. Curr. Protoc. Bioinform. 36:10.7.1‐10.7.20. © 2011 by John Wiley & Sons, Inc.

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